【摘要】：研究目的:用低劑量NaAsO_2長期誘導人骨髓間充質(zhì)干細胞(mesenchymal stem cells,MSCs),獲得抗砷細胞,探討機體抗砷機制。 研究方法:通過比較不同年齡人骨髓MSCs細胞倍增時間,細胞貼壁率等生物學性狀及細胞的砷耐受性,篩選抗砷種子細胞;選取胎兒骨髓MSCs,采用細胞抑制率在5%-10~時的NaAsO_2濃度(1μmol/L)為砷誘導濃度,同時設一組不加砷培養(yǎng)的胎兒骨髓MSCs作為同步對照,用噻唑蘭法(MTT)監(jiān)測細胞抗砷性是否產(chǎn)生;采用氫化物發(fā)生—原子熒光方法檢測細胞砷含量,二硫代二硝基苯甲酸直接顯色法,比較抗砷細胞與同步對照組胞內(nèi)GSH,GST等砷代謝指標的變化,驗證細胞是否獲得抗砷性,并初步探討了細胞產(chǎn)生抗砷能力的機制。 結(jié)果:不同年齡人骨髓MSCs在細胞形態(tài)、生長特性等方面是相似的,胎兒骨髓MSCs的擴增潛能明顯強于成人和老人骨髓MSCs。選擇胎兒骨髓MSCs作為抗砷種子細胞,經(jīng)過18周NaAsO_2誘導后,細胞對急性砷中毒的耐受性明顯提高,排砷能力增強,細胞內(nèi)GSH和GST活性增高。 結(jié)論:胎兒骨髓MSCs體外增殖分裂能力最強。人骨髓MSCs在低劑量NaAsO_2長期誘導下,能分化為具有抗砷能力的細胞。
[Abstract]:Objective: to obtain anti-arsenic cells from human bone marrow mesenchymal stem cells (mesenchymal stem cells,MSCs) induced by low dose NaAsO_2 for a long time, and to explore the mechanism of arsenic resistance. Methods: the anti-arsenic seed cells were screened by comparing the biological characteristics such as doubling time, cell adhesion rate and arsenic tolerance of human bone marrow MSCs cells of different ages. Fetal bone marrow MSCs, was treated with NaAsO_2 concentration (1 渭 mol / L) when the cell inhibition rate was 5% 鈮，

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