【摘要】：研究目的:用低劑量NaAsO_2長(zhǎng)期誘導(dǎo)人骨髓間充質(zhì)干細(xì)胞(mesenchymal stem cells,MSCs),獲得抗砷細(xì)胞,探討機(jī)體抗砷機(jī)制。 研究方法:通過(guò)比較不同年齡人骨髓MSCs細(xì)胞倍增時(shí)間,細(xì)胞貼壁率等生物學(xué)性狀及細(xì)胞的砷耐受性,篩選抗砷種子細(xì)胞;選取胎兒骨髓MSCs,采用細(xì)胞抑制率在5%-10~時(shí)的NaAsO_2濃度(1μmol/L)為砷誘導(dǎo)濃度,同時(shí)設(shè)一組不加砷培養(yǎng)的胎兒骨髓MSCs作為同步對(duì)照,用噻唑蘭法(MTT)監(jiān)測(cè)細(xì)胞抗砷性是否產(chǎn)生;采用氫化物發(fā)生—原子熒光方法檢測(cè)細(xì)胞砷含量,二硫代二硝基苯甲酸直接顯色法,比較抗砷細(xì)胞與同步對(duì)照組胞內(nèi)GSH,GST等砷代謝指標(biāo)的變化,驗(yàn)證細(xì)胞是否獲得抗砷性,并初步探討了細(xì)胞產(chǎn)生抗砷能力的機(jī)制。 結(jié)果:不同年齡人骨髓MSCs在細(xì)胞形態(tài)、生長(zhǎng)特性等方面是相似的,胎兒骨髓MSCs的擴(kuò)增潛能明顯強(qiáng)于成人和老人骨髓MSCs。選擇胎兒骨髓MSCs作為抗砷種子細(xì)胞,經(jīng)過(guò)18周NaAsO_2誘導(dǎo)后,細(xì)胞對(duì)急性砷中毒的耐受性明顯提高,排砷能力增強(qiáng),細(xì)胞內(nèi)GSH和GST活性增高。 結(jié)論:胎兒骨髓MSCs體外增殖分裂能力最強(qiáng)。人骨髓MSCs在低劑量NaAsO_2長(zhǎng)期誘導(dǎo)下,能分化為具有抗砷能力的細(xì)胞。
[Abstract]:Objective: to obtain anti-arsenic cells from human bone marrow mesenchymal stem cells (mesenchymal stem cells,MSCs) induced by low dose NaAsO_2 for a long time, and to explore the mechanism of arsenic resistance. Methods: the anti-arsenic seed cells were screened by comparing the biological characteristics such as doubling time, cell adhesion rate and arsenic tolerance of human bone marrow MSCs cells of different ages. Fetal bone marrow MSCs, was treated with NaAsO_2 concentration (1 渭 mol / L) when the cell inhibition rate was 5% 鈮，

本文編號(hào)：2509177