【摘要】：目的:研究主要組織相容性復(fù)合體(Major histocompatibility complex,MHC)Ⅰ類(lèi)分子不同的腫瘤細(xì)胞在與其MHC分子匹配/非匹配小鼠中的移植瘤成瘤情況,探索MHC分子在腫瘤發(fā)生中的作用。方法:1×104、3×104、5×104個(gè)小鼠結(jié)腸癌細(xì)胞CT26(H2d)分別接種BALB/c(H2d)和C57BL/6(H2b)小鼠,每個(gè)劑量對(duì)應(yīng)設(shè)置皮下組和靜脈組,對(duì)C57BL/6小鼠增加5×106、8×106和1×107數(shù)量組別;同樣將1×105、5×105、1×106個(gè)小鼠黑色素瘤細(xì)胞B16F10(H2b)分別經(jīng)皮下或靜脈接種到兩種小鼠體內(nèi),以相同接種量和接種方式下的不同小鼠間形成對(duì)比,14 d后觀(guān)察各組成瘤情況。結(jié)果:在BALB/c小鼠成瘤實(shí)驗(yàn)中,CT26細(xì)胞數(shù)為1×104時(shí)靜脈和皮下組均不成瘤,3×104時(shí)靜脈組部分成瘤,皮下組不成瘤;5×104時(shí)兩個(gè)組全部成瘤;注射1×105B16F10細(xì)胞時(shí)兩組均不成瘤,5×105時(shí)靜脈組全部成瘤,皮下組部分成瘤,1×106時(shí)兩組全部成瘤。在C57BL/6小鼠實(shí)驗(yàn)中,皮下注射CT26細(xì)胞數(shù)5×106時(shí)不成瘤,6×106和8×106部分成瘤,1×107時(shí)皮下組全部成瘤,靜脈組中CT26細(xì)胞達(dá)5×106也無(wú)腫瘤形成,大于此數(shù)量細(xì)胞注射時(shí)小鼠會(huì)因?yàn)榉嗡ㄈ劳?對(duì)B16F10細(xì)胞,1×105時(shí)靜脈和皮下組即可全部成瘤。結(jié)論:當(dāng)腫瘤細(xì)胞與小鼠MHCⅠ表型相匹配,移植瘤成瘤所需細(xì)胞數(shù)遠(yuǎn)小于MHCⅠ表型不相匹配所需細(xì)胞數(shù),說(shuō)明MHC分子對(duì)腫瘤發(fā)生存在影響,此種差異為研究MHC與腫瘤的關(guān)系提供了線(xiàn)索,同時(shí)也提供了CT26、B16F10細(xì)胞株成瘤實(shí)驗(yàn)的精確數(shù)據(jù)。
[Abstract]:Aim: to study the tumorigenesis of tumor cells with different class I molecules of (Major histocompatibility complex,MHC) in mice matched with their MHC molecules, and to explore the role of MHC molecules in tumorigenesis. Methods: 1 脳 10 ~ 4, 3 脳 10 ~ 4, 5 脳 10 ~ 4 mice colon cancer cell line CT26 (H _ 2d) were inoculated with BALB/c (H _ 2d) and C57BL/6 (H _ 2B) mice, respectively. the subcutaneous group and vein group were set up for each dose, and the C57BL/6 mice were increased by 5 脳 10 ~ 6, 8 脳 10 ~ 6 and 1 脳 10 ~ 7 groups, respectively. At the same time, 1 脳 10 ~ 5, 5 脳 10 ~ 5 and 1 脳 10 ~ 6 mouse melanoma cells B16F10 (H _ 2b) were inoculated subcutaneously or intravenously into two kinds of mice, and the mice were compared with each other under the same inoculum size and vaccination mode. The tumor formation was observed 14 days later. Results: in the tumorigenesis experiment of BALB/c mice, there was no tumor in vein and subcutaneous group when the number of CT26 cells was 1 脳 10 ~ 4, partial tumorigenesis in vein group at 3 脳 10 ~ 4, no tumor in subcutaneous group at 5 脳 10 ~ 4, no tumor in both groups at 5 脳 10 ~ 4, no tumor in 5 脳 10 ~ 5 vein group, partial tumorigenesis in subcutaneous group and tumor formation in 1 脳 10 ~ 6 group. In the experiment of C57BL/6 mice, the number of CT26 cells injected subcutaneously was 5 脳 106, 6 脳 106 and 8 脳 106 were partial tumorigenesis, all tumors were formed in subcutaneous group at 1 脳 107, and there was no tumor formation in venous group when CT26 cells reached 5 脳 106, mice would die of pulmonary embolism when larger than this number of cells injection, and all of B16F10 cells could be formed in 1 脳 10 5 vein and subcutaneous group. Conclusion: when the tumor cells match the MHC 鈪，

本文編號(hào)：2501325