兼性胞內(nèi)寄生菌HLK1～T對人宿主細胞的感染和持續(xù)性寄生的研究
發(fā)布時間：2019-06-14 16:17
【摘要】：目前,病原菌仍是人類健康最大的威脅之一,比如急性呼吸道感染、腹瀉、肺結(jié)核等疾病都是由于病原菌的感染所致。而且,新的細菌感染性疾病的不斷出現(xiàn),以及大量致病菌的反復(fù)出現(xiàn)和耐藥性的增加,對公眾健康造成可怕的威脅。因此,對潛在病原菌的發(fā)現(xiàn)以及病原菌致病機理的研究一直是研究的熱點之一。 人紅白血病細胞系K562細胞被廣泛用于實驗室的研究。當(dāng)我們對K562細胞進行核型分析過程中,意外地發(fā)現(xiàn)K562細胞染色標(biāo)本中有桿狀細菌樣的結(jié)構(gòu)。為了排除該實驗中相關(guān)實驗試劑的環(huán)境細菌污染,我們嚴格控制了各種實驗試劑和實驗操作。然而,多次重復(fù)實驗仍得到以上結(jié)果。因此,我們推論該細胞中可能存在一種細胞內(nèi)生菌。將K562細胞、RPMI 1640完全培養(yǎng)基以及各種實驗用試劑分別涂至基礎(chǔ)血平板(5%綿羊血)上,置37℃孵箱中培養(yǎng)48～72小時,結(jié)果發(fā)現(xiàn)涂有K562細胞的平板上有菌落生成,而其它平板均無任何菌落形成。經(jīng)多次實驗證明,平板上形成的細菌集落確為K562細胞來源。只得注意的是,K562細胞能保持良好的生長狀態(tài)和增殖能力,而且細胞培養(yǎng)基中無明顯的細菌污染跡象。以上初步的實驗結(jié)果提示,寄生在K562細胞內(nèi)的細菌可能是一種新的細胞內(nèi)生菌,因為目前已明確的能寄生在人類細胞內(nèi)的胞內(nèi)寄生菌在細胞內(nèi)會大量繁殖,因此感染的細胞不能健康生長。由于該菌可能是一種新的胞內(nèi)寄生菌,就有必要明確該菌在細菌分類中的地位,以及該菌的基本的生物學(xué)特性。 第一部分:HLK1~T的分離和鑒定 目的:對細菌的分類鑒定是了解其生物學(xué)功能和特性的基礎(chǔ)。為明確HLK1~T的分類地位及其基本生物學(xué)特性,該研究從人紅白血病細胞系562細胞中分離獲得
[Abstract]:At present, the pathogenic bacteria are still one of the greatest threats to human health, such as acute respiratory tract infection, diarrhea, pulmonary tuberculosis and other diseases due to the infection of the pathogenic bacteria. Moreover, the emergence of new bacterial infectious diseases, as well as the repeated appearance and resistance of a large number of pathogenic bacteria, poses a terrible threat to public health. Therefore, the research on the discovery of the potential pathogenic bacteria and the pathogenesis of the pathogenic bacteria has been one of the hot spots of the study. Human erythroleukemia cell line K562 cells are widely used It was used in the research of the laboratory. In the process of the karyotype analysis of K562 cells, it was found that the K562 cells were stained with staining. The structure of the rod-shaped bacteria-like structure. In order to eliminate the environmental bacterial contamination of the relevant experimental reagents in the experiment, we have strictly controlled the various facts The test reagent and the experimental operation. However, the test is repeated many times. The above results are still available. Therefore, we can infer that the cells may be stored in the cell In a cell endophytic bacterium, K562 cells, RPMI 1640 full culture medium, and various experimental reagents are respectively coated on a basic blood plate (5% sheep blood), and incubated for 48-72 hours in a 37-degree-C cold box, and the results show that the plate coated with K562 cells has colony formation, while other plates None of the colonies were formed. The colony of bacteria formed on the plate was proved to be true by multiple experiments. The K562 cell is a source of cell. It is important to note that K562 cells can maintain good growth and proliferation, and the cell culture medium is not clear. The above preliminary experimental results suggest that the bacteria that are parasitic in the K562 cells may be a new endogenetic strain, as the intracellular parasitic bacteria that can be parasitically in the human cell can be propagated in a large amount in the cell, thus infecting The cell cannot grow well. As the bacteria may be a new intracellular parasitic, it is necessary to identify the status of the bacterium in the bacterial classification, and the bacteria. Basic biological characteristics. Part 1: Separation and identification of HLK1-T Objective: The classification and identification of bacteria is the basis of understanding the biological functions and characteristics of the bacteria. In order to clarify the classification status of HLK1-T and its basic life
【學(xué)位授予單位】：浙江大學(xué)
【學(xué)位級別】：博士
【學(xué)位授予年份】：2006
【分類號】：R378
本文編號：2499517
[Abstract]:At present, the pathogenic bacteria are still one of the greatest threats to human health, such as acute respiratory tract infection, diarrhea, pulmonary tuberculosis and other diseases due to the infection of the pathogenic bacteria. Moreover, the emergence of new bacterial infectious diseases, as well as the repeated appearance and resistance of a large number of pathogenic bacteria, poses a terrible threat to public health. Therefore, the research on the discovery of the potential pathogenic bacteria and the pathogenesis of the pathogenic bacteria has been one of the hot spots of the study. Human erythroleukemia cell line K562 cells are widely used It was used in the research of the laboratory. In the process of the karyotype analysis of K562 cells, it was found that the K562 cells were stained with staining. The structure of the rod-shaped bacteria-like structure. In order to eliminate the environmental bacterial contamination of the relevant experimental reagents in the experiment, we have strictly controlled the various facts The test reagent and the experimental operation. However, the test is repeated many times. The above results are still available. Therefore, we can infer that the cells may be stored in the cell In a cell endophytic bacterium, K562 cells, RPMI 1640 full culture medium, and various experimental reagents are respectively coated on a basic blood plate (5% sheep blood), and incubated for 48-72 hours in a 37-degree-C cold box, and the results show that the plate coated with K562 cells has colony formation, while other plates None of the colonies were formed. The colony of bacteria formed on the plate was proved to be true by multiple experiments. The K562 cell is a source of cell. It is important to note that K562 cells can maintain good growth and proliferation, and the cell culture medium is not clear. The above preliminary experimental results suggest that the bacteria that are parasitic in the K562 cells may be a new endogenetic strain, as the intracellular parasitic bacteria that can be parasitically in the human cell can be propagated in a large amount in the cell, thus infecting The cell cannot grow well. As the bacteria may be a new intracellular parasitic, it is necessary to identify the status of the bacterium in the bacterial classification, and the bacteria. Basic biological characteristics. Part 1: Separation and identification of HLK1-T Objective: The classification and identification of bacteria is the basis of understanding the biological functions and characteristics of the bacteria. In order to clarify the classification status of HLK1-T and its basic life
【學(xué)位授予單位】：浙江大學(xué)
【學(xué)位級別】：博士
【學(xué)位授予年份】：2006
【分類號】：R378
【共引文獻】
相關(guān)期刊論文 前3條
1 陳炯;杜敏;;膽囊癌病因?qū)W研究進展[J];臨床誤診誤治;2007年03期
2 周海華;王建球;陳躍宇;楊廷燕;;感染與膽囊癌發(fā)病外文文獻的Meta分析[J];中華臨床醫(yī)師雜志(電子版);2011年06期
3 Gopal Nath;Anil K Gulati;Vijay K Shukla;;Role of bacteria in carcinogenesis, with special reference to carcinoma of the gallbladder[J];World Journal of Gastroenterology;2010年43期
相關(guān)博士學(xué)位論文 前3條
1 彭歡;TLR4信號通路在膽囊癌中的表達及其作用機制的初步研究[D];第二軍醫(yī)大學(xué);2011年
2 黃海林;膽囊癌組織相關(guān)蛋白的篩選與鑒定[D];第二軍醫(yī)大學(xué);2009年
3 牛超;未知細菌病原體毒力、重組篩查體系研究[D];中國人民解放軍軍事醫(yī)學(xué)科學(xué)院;2010年
相關(guān)碩士學(xué)位論文 前2條
1 雷曉婭;自然霉變玉米及甘露寡糖對仔豬生長性能和腸道健康的影響[D];四川農(nóng)業(yè)大學(xué);2011年
2 亞森江;原發(fā)性膽囊癌98例回顧性分析[D];新疆醫(yī)科大學(xué);2004年
，本文編號：2499517
本文鏈接：http://sikaile.net/yixuelunwen/binglixuelunwen/2499517.html
最近更新
教材專著
