【摘要】： 甘氨膽酸是膽汁酸的主要成分之一,屬于結(jié)合型膽汁酸,在肝臟中由膽酸和甘氨酸結(jié)合而成。多種動(dòng)物膽汁中均含有一定比例的甘氨膽酸。為了探索從牛膽汁中分離提取甘氨膽酸的工藝路線(xiàn),并了解甘氨膽酸對(duì)機(jī)體免疫功能的影響。采用鹽析、萃取、柱層析等生物化學(xué)方法,從牛膽汁中提取出了有效成分甘氨膽酸,并采用薄層層析法及薄層掃描法進(jìn)行定量、定性檢測(cè),測(cè)得甘氨膽酸的純度可達(dá)98%以上,牛膽汁甘氨膽酸的回收率為64%。采用sc環(huán)磷酰胺(CTX)及環(huán)孢菌素A(Cyclosporin A CsA)制造免疫抑制模型;采用ELISA及比色法觀(guān)察GCA對(duì)體液免疫的影響;碳廓清法測(cè)定了GCA對(duì)單核巨噬細(xì)胞的吞噬功能的影響;采用遲發(fā)型皮膚變態(tài)反應(yīng)法觀(guān)察GCA對(duì)二硝基氯苯(DNCB)丙酮溶液所致小鼠遲發(fā)型皮膚過(guò)敏反應(yīng)的影響,并測(cè)定了血清中IL-2、TNF-α的含量;采用流式細(xì)胞術(shù)檢測(cè)了GCA對(duì)小鼠外周血T細(xì)胞亞群CD4+、CD8+比例的影響。結(jié)果顯示GCA可以顯著增強(qiáng)正常及抑制小鼠的IgM、IgG的含量;顯著提高巨噬細(xì)胞的吞噬功能;顯著增加外周血中T淋巴細(xì)胞CD4+比例,使CD4+/CD8+比值提高;顯著抑制遲發(fā)型皮膚變態(tài)反應(yīng)的發(fā)生,并顯著增加IL-6和TNF-α的含量。表明,GCA對(duì)機(jī)體免疫功能具有調(diào)節(jié)作用。
[Abstract]:Glycine cholic acid is one of the main components of bile acid, which belongs to bound bile acid and is formed by the combination of cholic acid and glycine in the liver. Many kinds of animal bile contain a certain proportion of glycine cholic acid. In order to explore the separation and extraction of glycine cholic acid from bovine bile and to understand the effect of glycine cholic acid on immune function. Glycine cholic acid was extracted from bovine bile by salting out, extraction and column chromatography. The purity of glycine cholic acid was over 98% by thin layer chromatography and thin layer scanning. The recovery rate of glycine cholic acid in bovine bile was 64%. The immunosuppressive model was established by sc cyclophosphamide (CTX) and cyclosporine A (Cyclosporin A CsA), the effect of GCA on humoral immunity was observed by ELISA and colorimetric method, and the effect of GCA on the phagocytosis of monocytes was measured by carbon profile assay. The effect of GCA on delayed skin allergic reaction induced by dinitrochlorobenzene (DNCB) acetone solution in mice was observed by delayed skin allergy method, and the content of IL-2,TNF- 偽 in serum was determined. The effects of GCA on the ratio of CD4 and CD8 in peripheral blood T cell subsets of mice were detected by flow cytometry. The results showed that GCA could significantly enhance the content of IgM,IgG and inhibit the content of IgM,IgG in mice, significantly increase the phagocytic function of macrophages, significantly increase the CD4 ratio of T lymphocytes in peripheral blood, and increase the ratio of CD4 / CD8. It significantly inhibited the occurrence of delayed skin allergy and significantly increased the contents of IL-6 and TNF- 偽. The results showed that GCA could regulate the immune function of the body.
【學(xué)位授予單位】：內(nèi)蒙古農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2006
【分類(lèi)號(hào)】：R392

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 羅克;動(dòng)物的免疫器官、免疫細(xì)胞與免疫分子[J];福建畜牧獸醫(yī);2005年01期

2 劉建強(qiáng),張志堅(jiān);膽汁酸對(duì)肝硬化大鼠腸道細(xì)菌過(guò)度生長(zhǎng)、細(xì)菌移位和內(nèi)毒素血癥的影響[J];福建醫(yī)藥雜志;2005年03期

3 胡淼,錢(qián)國(guó)平,蘇寶根,鮑宗必,任其龍;硅膠柱層析純化青蒿素[J];華西藥學(xué)雜志;2005年04期

4 張曼,宋崇順,任映,郭菁,楊金鐸;鼻舒滴丸對(duì)變應(yīng)性鼻炎模型豚鼠T細(xì)胞亞群及相關(guān)細(xì)胞因子和IgE調(diào)節(jié)的影響[J];北京中醫(yī)藥大學(xué)學(xué)報(bào);2004年06期

5 郝麗莉,趙文靜,王亞威;動(dòng)物膽汁的藥用研究[J];中國(guó)民族民間醫(yī)藥雜志;1999年05期

6 陶文元,李霞;210例肝病患者血清甘氨膽酸測(cè)定[J];南京醫(yī)學(xué)院學(xué)報(bào);1993年04期

7 余上才，，章育正，趙慧娟，于麗華;枸杞子和白術(shù)免疫調(diào)節(jié)作用的實(shí)驗(yàn)研究[J];上海免疫學(xué)雜志;1994年01期

8 張東偉,陳錫美,陳林波;血清透明質(zhì)酸和甘氨膽酸聯(lián)合檢測(cè)肝硬化[J];上海鐵道大學(xué)學(xué)報(bào);1998年11期

9 王歡;喬模;;清熱抗感沖劑對(duì)免疫功能影響的實(shí)驗(yàn)研究[J];上海中醫(yī)藥大學(xué)學(xué)報(bào);2005年04期

10 李爍,張志文,管又飛;膽汁酸受體FXR的研究進(jìn)展[J];生理科學(xué)進(jìn)展;2003年04期

相關(guān)碩士學(xué)位論文 前1條

1 井歡;香菇多糖對(duì)脾虛小鼠免疫功能的調(diào)節(jié)作用[D];遼寧中醫(yī)學(xué)院;2003年

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