【摘要】：目的了解福建省結(jié)核分枝桿菌異煙肼耐藥相關(guān)基因的突變特征,為異煙肼耐藥快速檢測(cè)方法的建立提供一定的科學(xué)依據(jù)。方法對(duì)來源于福建省結(jié)核病耐藥性監(jiān)測(cè)30個(gè)監(jiān)測(cè)點(diǎn)納入的75株耐多藥和10株全敏感結(jié)核分枝桿菌分離株,進(jìn)行katG、inhA、oxyR-ahpC基因片段PCR擴(kuò)增并測(cè)序分析,用RD105缺失基因檢測(cè)法進(jìn)行北京家族基因型鑒定,使用卡方檢驗(yàn)分析相關(guān)性。結(jié)果 10株全敏感株未檢測(cè)到突變。75株耐多藥結(jié)核分枝桿菌檢測(cè)到72株katG、inhA、oxyR-ahpC發(fā)生單一或聯(lián)合基因突變,突變率為96.0%(72/75)。其中,65株(86.7%,65/75)發(fā)生katG突變,涉及5個(gè)位點(diǎn),最常見位點(diǎn)突變的密碼子是315,突變率為82.7%(62/75),最常見突變形式為Ser315Thr(77.3%,58/75);8株(10.7%,8/75)發(fā)生inhA突變,突變形式均為C(-15)T;5株(6.7%,5/75)發(fā)生oxyR-ahpC突變,突變形式為C(-39)T或C(-46)A。katG、inhA和oxyR-ahpC在北京家族基因型菌株和非北京家族基因型菌株中的突變率分別為83.9%(47/56)、12.5%(7/56)、7.1%(4/56)和94.7%(18/19)、5.3%(1/19)、5.3%(1/19),差異無統(tǒng)計(jì)學(xué)意義(P值分別為0.23、0.38、0.78)。結(jié)論福建省結(jié)核分枝桿菌異煙肼耐藥性相關(guān)基因突變絕大多數(shù)發(fā)生在katG、inhA和oxyR-ahpC基因位點(diǎn),且以katG突變?yōu)橹�。初步分析顯示北京家族基因型菌株流行與異煙肼耐藥基因突變特征無關(guān)。
[Abstract]:Objective to investigate the mutation characteristics of isoniazid resistance related genes in Mycobacterium tuberculosis in Fujian Province, and to provide some scientific basis for the establishment of rapid detection method for isoniazid resistance. Methods 75 strains of multi-drug resistance and 10 strains of fully sensitive Mycobacterium tuberculosis from 30 surveillance sites of tuberculosis resistance in Fujian Province were amplified by PCR and sequenced. The Beijing family genotyping was identified by RD105 deletion gene detection, and the correlation was analyzed by chi-square test. Results No mutation was detected in 10 fully sensitive strains. 72 strains of katG,inhA,oxyR-ahpC were detected to have single or combined gene mutation, the mutation rate was 96.0% (72 鈮，

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