【摘要】：[目的] 觀察和比較家兔在同等條件下分別感染日本血吸蟲云南品系和皖鄂品系后，其肝功能的動態(tài)變化以及肝腸病理學(xué)改變和異位寄生情況，探討不同地域的兩種品系日本血吸蟲對宿主致病性的差異。 [方法] 22只成年健康家兔隨機分為對照組、大理組和皖鄂組，分別用大理釘螺和江蘇釘螺逸出的血吸蟲尾蚴(150±2條)以腹部貼片法攻擊感染。用全自動生化分析儀于感染后不同時間分別檢測對照組和實驗組家兔血清總蛋白，白蛋白和球蛋白，丙氨酸氨基轉(zhuǎn)移酶(ALT)，天冬氨酸氨基轉(zhuǎn)移酶(AST)及血清總膽紅素和γ-谷氨酰轉(zhuǎn)肽酶(γ-GT)等肝功能指標，并進行統(tǒng)計分析；于感染后不同時間分別剖殺實驗動物，觀察肝表面蟲卵結(jié)節(jié)的密度及大小；在光學(xué)顯微鏡下觀察肝臟、小腸和大腸等組織的病理學(xué)變化；用放射免疫法(RIA)檢測感染后12W各組家兔血清透明質(zhì)酸(HA)，Ⅲ型前膠原(PCⅢ)，Ⅳ型膠原(Ⅳ-C)等肝纖維化指標；壓片觀察肺臟和腦組織觀察異位寄生情況。 [結(jié)果] 本實驗中，實驗組家兔血清白蛋白持續(xù)下降，而血清球蛋白持續(xù)上升，整個實驗過程中血清總蛋白無明顯變化，兩種轉(zhuǎn)氨酶及γ-谷氨酰轉(zhuǎn)肽酶活性都以增高——進一步增高——恢復(fù)的形式變化，膽紅素則一直升高。除膽紅素外，其它的肝功能指標變化均以大理組為著。肝臟結(jié)構(gòu)損害主要表現(xiàn)為：感染后2周，肝血竇擴張充血，肝細胞水腫，，少數(shù)肝細胞脂肪變性；感染后6周，門脈區(qū)有充血及炎細胞浸潤，大部分成熟蟲卵周圍均有密集的以嗜酸性粒細胞為主的炎細胞浸潤，有的則因炎細胞壞死崩解而形成急性蟲卵膿腫；感染后12周，未見假小葉形成，匯管區(qū)有較多的蟲卵結(jié)節(jié)，伴有纖維組織增生及慢性炎細胞浸潤，小腸、結(jié)腸的病理改變?yōu)檫M行性加重的炎癥反應(yīng)，結(jié)腸可見不同時期的急性、
[Abstract]:[objective] to observe and compare the dynamic changes of liver function, pathological changes of liver and intestine and ectopic parasitism in rabbits infected with Schistosoma japonicum Yunnan strain and Anhui-Hubei strain under the same conditions, respectively. The pathogenicity of two strains of Schistosoma japonicum (Schistosoma japonicum) from different regions to host was studied. [methods] Twenty-two adult healthy rabbits were randomly divided into three groups: control group, Dali group and Anhui-Hubei group. The infected rabbits were attacked by abdominal patch method with schistosoma cercariae (150 鹵2) escaped from Oncomelania dali and Oncomelania hupensis respectively. The serum total protein, albumin, globulin and alanine aminotransferase (ALT), in rabbits of control group and experimental group were detected by automatic biochemical analyzer at different time after infection. The liver function indexes, such as aspartate aminotransferase (AST), serum total bilirubin and 緯-glutamyl transpeptidase (緯-GT), were analyzed statistically. The density and size of egg nodules on the surface of liver were observed, and the pathological changes of liver, small intestine and large intestine were observed under optical microscope, and the pathological changes of liver, small intestine and large intestine were observed at different time after infection. Serum hyaluronic acid (HA), 鈪

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