【摘要】：目的:一、建立大鼠缺血性結(jié)腸炎模型并探討其發(fā)病機(jī)制。 二、使用不同劑量的脂微球前列腺素E1(Lipo-PGE1)對(duì)缺血性結(jié)腸炎大鼠進(jìn)行治療,評(píng)價(jià)其治療效果及探討治療機(jī)制,為臨床用藥提供理論依據(jù)。 三、使用不同劑量的阿魏酸鈉(SF)對(duì)缺血性結(jié)腸炎大鼠進(jìn)行治療,評(píng)價(jià)SF治療效果及探討治療機(jī)制,為治療缺血性結(jié)腸炎提供新的途徑。 方法:一、光化學(xué)法制備大鼠缺血性結(jié)腸炎模型:靜脈注射光敏劑PSD-007后,用激光照射近端結(jié)腸。分別在照射后0、1、3、5、7、14天等不同時(shí)間點(diǎn)處死大鼠。檢測(cè)結(jié)腸粘膜組織的LI、SOD、MDA和血清TNF-α的動(dòng)態(tài)變化。 二、用不同劑量Lipo-PGEl治療缺血性結(jié)腸炎大鼠:對(duì)照組(生理鹽水)、小劑量組(0.5ug/kg)、中劑量組(1ug/kg)、大劑量組(2ug/kg)。各組均連續(xù)用藥五天后全部處死,檢測(cè)LI、SOD、MDA和6-keto-PGFl_α、TXB2及6-keto-PGFl_α/TXB2的變化,分析TNF-α、ICAM-1在結(jié)腸組織的表達(dá)情況。 三、用不同劑量的SF來治療缺血性結(jié)腸炎大鼠,分為:對(duì)照組(生理鹽水)、小劑量組(20mg/kg),中劑量組(40mg/kg)、大劑量組(80mg/kg),各組均連續(xù)用藥五天后全部處死,檢測(cè)LI、ET-1、SOD、MDA和6-keto-PGFl_α、TXB2及6-keto-PGFl_α/TXB2的變化,分析TNF-α、ICAM-1在結(jié)腸組織的表達(dá)情況。 結(jié)果:一、模型組大鼠經(jīng)激光照射后出現(xiàn)進(jìn)食水減少、活動(dòng)度減弱、排便增多及暗紅色血便等表現(xiàn),LI隨時(shí)間增加而加重,第5天達(dá)到最高點(diǎn)(3.25±0.46,P0.01),之后逐步下降,到第14天基本恢復(fù)正常;SOD活性隨時(shí)間而下降,第3天到達(dá)最低點(diǎn)(19.12±6.70,P0.01),MDA含量隨時(shí)間而增加,第3天到達(dá)最高點(diǎn)(30.38±4.41,P0.01);TNF-α含量隨時(shí)間而增加,第5天到達(dá)最高點(diǎn)(1.89±0.38,P0.01)。上述指標(biāo)均在第14天恢復(fù)正常。SOD與LI呈負(fù)相關(guān),相關(guān)系數(shù)-0.7223(P0.01);MDA、TNF-α與LI呈正相關(guān),
[Abstract]:Objective: 1. To establish a rat model of ischemic colitis and to explore its pathogenesis. Second, different doses of lipid microsphere prostaglandin E1 (Lipo-PGE1) were used to treat ischemic colitis rats, to evaluate the therapeutic effect and explore the therapeutic mechanism, to provide a theoretical basis for clinical use of drugs. Third, different doses of sodium ferulate (SF) were used to treat ischemic colitis rats, to evaluate the therapeutic effect of SF and explore the therapeutic mechanism, so as to provide a new way for the treatment of ischemic colitis. Methods: 1. The model of ischemic colitis in rats was made by photochemical method. After intravenous injection of photosensitizer PSD-007, the proximal colon was irradiated by laser. Rats were killed at 0, 1, 3, 5, 7, 14 days after irradiation. The dynamic changes of LI,SOD,MDA and serum TNF- 偽 in colonic mucosa were detected. Two, rats with ischemic colitis were treated with different doses of Lipo-PGEl: control group (saline), low dose group (0.5ug/kg), middle dose group (1ug/kg), high dose group (2ug/kg). All the rats in each group were killed for 5 days. The changes of LI,SOD,MDA, 6-keto-PGFL _ 偽, TXB2 and 6-keto-PGFL _ 偽 / TXB2 were detected, and the expression of TNF- 偽 and ICAM-1 in colon tissue were analyzed. The rats with ischemic colitis were divided into three groups: control group (saline), low dose group (20mg/kg), middle dose group (40mg/kg) and high dose group (80mg/kg). All the rats in each group were killed for 5 days. The changes of LI,ET-1,SOD,MDA, 6-keto-PGFL _ 偽, TXB2 and 6-keto-PGFL _ 偽 / TXB2 were detected, and the expression of TNF- 偽 and ICAM-1 in colon tissue were analyzed. Results: 1. After laser irradiation, the rats in the model group showed decreased intake of water, decreased activity, increased defecation and dark red blood stool. LI increased with the increase of time and reached the highest point on the 5th day (3.25 鹵0.46, P0.01). After that, it gradually decreased and returned to normal on the 14th day. The activity of SOD decreased with time and reached the lowest point on the third day (19.12 鹵6.70, P0.01). The content of), MDA increased with time and reached the highest point on the third day (30.38 鹵4.41, P0.01). The content of TNF- 偽 increased with time and reached the highest point on the 5th day (1.89 鹵0.38, P0.01). Sod was negatively correlated with LI, the correlation coefficient was-0.7223 (P0.01), and MDA,TNF- 偽 was positively correlated with LI.
【學(xué)位授予單位】：中國(guó)人民解放軍軍醫(yī)進(jìn)修學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2006
【分類號(hào)】：R574.62;R-332

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 許德余,殷祥生,陳雄,陳文暉,劉軍;腫瘤光敏診治藥物的研究——Ⅱ、新光敏劑PSD-007的研究[J];第二軍醫(yī)大學(xué)學(xué)報(bào);1984年01期

2 孫春亮,季兵,黎介壽,張新華,朱維銘,汪俊軍;前列腺素E_1治療大鼠胰腺炎肺損傷的作用機(jī)制[J];肝膽胰外科雜志;2001年02期

3 劉寶華;內(nèi)皮素的血管生物學(xué)效應(yīng)研究進(jìn)展[J];國(guó)外醫(yī)學(xué)(創(chuàng)傷與外科基本問題分冊(cè));1996年02期

4 劉夢(mèng)瓊 ,鄭義;阿魏酸鈉對(duì)氧化低密度脂蛋白所致的內(nèi)皮細(xì)胞釋放NO的影響[J];華中醫(yī)學(xué)雜志;2002年01期

5 金惠銘,劉清行,曹翔,吳朝暉,張國(guó)平,張明,沙志一;TNF-α引起的微血管內(nèi)皮細(xì)胞功能障礙及其細(xì)胞分子機(jī)制[J];微循環(huán)學(xué)雜志;2000年03期

6 嚴(yán)明,歐陽靜萍,劉永明,涂淑珍,王保華,楊靜薇;阿魏酸鈉對(duì)TNF-α致內(nèi)皮細(xì)胞NF-κB和ICAM-1表達(dá)的影響[J];微循環(huán)學(xué)雜志;2001年04期

7 趙同峰,鄧華聰;阿魏酸鈉的藥理研究新進(jìn)展[J];西北藥學(xué)雜志;2001年03期

8 劉少平,董衛(wèi)國(guó),吳東方,羅和生,于皆平,梅俏;阿魏酸鈉對(duì)結(jié)腸炎大鼠結(jié)腸組織一氧化氮合酶和環(huán)氧合酶的影響[J];中國(guó)藥理學(xué)通報(bào);2003年05期

9 王峰,劉敏,楊連春,王京媛,蔡慶,呂敏,李菲;咖啡酸、阿魏酸:新的非肽類內(nèi)皮素拮抗劑[J];中國(guó)臨床藥理學(xué)與治療學(xué)雜志;1999年02期

10 丁明玉,馬帥武,劉德麟;阿魏酸的穩(wěn)定性及其在川芎和當(dāng)歸藥材中的存在形式[J];中草藥;2004年01期

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