發(fā)布時間：2019-04-22 14:15

【摘要】：目的:探討一種新的獲得IgY多克隆抗體的方法和效果。方法:用三種常見的細(xì)菌病原體金黃色葡萄球菌(SA)、大腸埃希氏菌(EC)、銅綠假單胞菌(PA)制備復(fù)合抗原,并經(jīng)過滅活后免疫來亨蛋雞產(chǎn)生IgY多克隆抗體。從免疫后的蛋中提取出IgY多克隆抗體,ELISA法測IgY抗體特異性及濃度滴度。SA、EC、PA三種標(biāo)準(zhǔn)菌株體外抑制實(shí)驗(yàn)檢測IgY抗體抑菌效果。結(jié)果:用SA、EC、PA復(fù)合抗原成功地免疫來亨蛋雞產(chǎn)生IgY多克隆抗體,ELISA法檢測抗體濃度滴度為100,000;IgY抗體明顯抑制SA、EC、PA三種標(biāo)準(zhǔn)菌株的生長。結(jié)論:SA、EC、PA三種細(xì)菌復(fù)合抗原可以刺激來亨蛋雞產(chǎn)生高濃度滴度抗體IgY,該抗體能明顯抑制SA、EC、PA三種標(biāo)準(zhǔn)菌株的生長。
[Abstract]:Objective: to explore a new method for obtaining polyclonal antibody against IgY. Methods: three common bacterial pathogens, Staphylococcus aureus (SA), Escherichia coli, (EC), Pseudomonas aeruginosa (PA), were used to prepare the complex antigen. After inactivation, IgY polyclonal antibody was produced by immunizing the egg hens. The polyclonal antibody against IgY was extracted from the immunized eggs. The specificity and concentration titers of IgY antibody were determined by ELISA method. The inhibitory effect of IgY antibody was detected by in vitro inhibition test of SA and EC,PA. Results: SA,EC,PA complex antigen was successfully used to immunize Leghorn laying hens to produce polyclonal antibodies against IgY. ELISA assay showed that the titer of antibody was 1 000 000 and IGY antibody significantly inhibited the growth of the three standard strains of SA,EC,PA. Conclusion: the three bacterial complex antigens of SA,EC,PA can stimulate Leghorn laying hens to produce high titer antibody IgY, which can significantly inhibit the growth of the three standard strains of SA,EC,PA.
【作者單位】： 中山大學(xué)孫逸仙紀(jì)念醫(yī)院檢驗(yàn)科;
【基金】：廣東省自然科學(xué)基金資助項(xiàng)目(S2013010014007) 廣東省科技計(jì)劃項(xiàng)目資助課題(2010A030400006)
【分類號】：R392

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