【摘要】：目的 本研究設(shè)計慢速程序冷凍法凍融人成熟卵母細胞(MII期)與未成熟卵母細胞(GV/MI期),分析凍融后卵母細胞的發(fā)育潛能及影響卵母細胞凍融后發(fā)育潛能的原因,進而確立“冷凍程序—卵母細胞所處階段—受精能力及早期胚胎的發(fā)育能力”三者間的聯(lián)系,旨在探索出一條適合人卵母細胞的凍融技術(shù)。 方法 將來源于行IVF-ET患者捐贈的67枚多余成熟卵母細胞(MII期)隨機分為A、B、C三組,在不同條件下進行慢速冷凍法凍融。根據(jù)CPA及解凍液中不同的SSS濃度和不同的去除卵母細胞周圍部分顆粒細胞的方法將卵母細胞分組如下:A組:24枚,15%SSS、透明質(zhì)酸酶消化法去顆粒;B組:21枚,30%SSS、透明質(zhì)酸酶消化法去顆粒;C組:22枚,30%SSS、機械法去顆粒。另隨機獲取同時期行IVF-ET中的新鮮卵母細胞(未冷凍)25枚作為對照。比較SSS濃度和去顆粒方法的不同對凍融后的卵母細胞存活率、受精能力及胚胎早期發(fā)育力的影響。 將來源于手術(shù)中卵巢組織的82枚人未成熟卵母細胞(GV/MI期)隨機分為兩組,凍融組57枚,新鮮對照組25枚。將凍融組卵母細胞慢速冷凍,凍融后存
[Abstract]:Objective to design a slow programmed freezing and thawing method for human mature oocytes (MII phase) and immature oocytes (GV/MI phase). This paper analyzes the developmental potential of oocytes after freezing and thawing and the reasons that affect the developmental potential of oocytes after freezing and thawing, and then establishes the relationship among "freezing procedure-stage of oocytes-fertilization ability and developmental ability of early embryos". In order to explore a suitable freezing and thawing technology for human oocytes. Methods 67 superfluous mature oocytes (MII phase) from IVF-ET patients were randomly divided into three groups: group A, group B, and group C, which were frozen and thawed by slow freezing under different conditions. According to the different concentration of SSS in CPA solution and different methods of removing granulosa cells around oocytes, oocytes were divided into the following groups: group A: 24 cells, 15% SSS, degranulation by hyaluronidase digestion; Group B: 21, 30% SSS, hyaluronidase digestion degranulation, group C: 22, 30% SSS, mechanical degranulation. In addition, 25 fresh oocytes (unfrozen) from simultaneous IVF-ET were randomly obtained as control. The effects of different concentrations of SSS and degranulation methods on the survival rate, fertilization ability and early embryo development ability of frozen-thawed oocytes were compared. In the future, 82 human immature oocytes (GV/MI phase) derived from ovarian tissue during operation were randomly divided into two groups: freeze-thaw group (57) and fresh control group (25). The oocytes in the freeze-thaw group were frozen slowly and stored after freezing and thawing.
【學(xué)位授予單位】：安徽醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2005
【分類號】：R321

【引證文獻】

相關(guān)碩士學(xué)位論文 前1條

1 邢瓊;雌性配子與人類三原核囊胚的玻璃化冷凍研究[D];安徽醫(yī)科大學(xué);2007年

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本文編號：2458422