【摘要】： [目的]調(diào)查云南省紅河州與保山市并殖吸蟲蟲種分布情況，從不同宿主體內(nèi)獲取病原體，觀察測量兩地并殖吸蟲的囊蚴、后尾蚴、蟲卵及成蟲的形態(tài)；比較兩種實驗終宿主—大鼠和家貓對兩地并殖吸蟲的易感性，并對兩地并殖吸蟲的成蟲進(jìn)行掃描電鏡觀察。確定兩地并殖吸蟲的種屬關(guān)系、宿主的適應(yīng)性以及第二中間宿主的感染情況，為云南省并殖吸蟲的研究和防治提供有用的科學(xué)依據(jù)。 [方法]分別從云南省紅河州金平縣和保山市采集溪蟹，分離囊蚴，形態(tài)觀察測量后，經(jīng)口飼感染大鼠和家貓，收集蟲體和蟲卵，將蟲體和脫囊后尾蚴制成染色封片標(biāo)本進(jìn)行形態(tài)觀測，并對成蟲進(jìn)行掃描電鏡觀察。 [結(jié)果]金平和保山囊蚴形態(tài)相似，為單層囊壁，，類圓形，大小分別為0.401 mm×0.395 mm和0.435 mm×0.433 mm。成蟲為長橢圓形，大小分別為10.107 mm×4.064mm和9.594 mm×3.577 mm，寬長比例分別為1：2.49和1：2.63，腹吸盤大于口吸盤，卵巢靠近腹吸盤位置，分葉簡單，次級分葉少見，睪丸中心體較小，其長度為蟲體長度的1／6。成蟲皮棘以單生型為主，腹吸盤前的皮棘多呈三角形或尖刀狀，腹吸盤后的皮棘末端呈弧形，少數(shù)出現(xiàn)分叉。兩地并殖吸蟲的囊蚴、后尾蚴、蟲卵和成蟲的形態(tài)基本相似，但前三者的大小存在差異，而成蟲則無差異。 金平溪蟹和保山溪蟹的感染率分別為79.9％和100％，金平溪蟹的每蟹平均囊蚴數(shù)和每克蟹重平均囊蚴數(shù)分別是1.8個和0.7個，保山溪蟹的海蟹平均囊蚴數(shù)和每克蟹重平均囊蚴數(shù)分別是13.3個和2個。金平囊蚴感染大鼠和家貓后蟲體回收率分別是24％和55％，成蟲回收率為17％和51.7％，保山囊蚴感染大鼠和家貓后蟲體回收率分別是5％和61.7％，成蟲回收率是4％和51.7％。 [結(jié)論]云南省紅河州金平縣和保山市均是并殖吸蟲流行區(qū)，保山溪蟹感染率和感染度均明顯高于金平溪蟹。金平縣和保山市流行的并殖吸蟲為同一蟲種，即陳氏并殖吸蟲，但不同地域之間存在形態(tài)學(xué)差異。大鼠對金平并殖吸蟲的易感性高于保山，家貓對兩地并殖吸蟲的易感性相似。兩種實驗終宿主比較，家貓的易感性明顯高于大鼠，家貓是陳氏并殖吸蟲理想的動物模型。
[Abstract]:[objective] to investigate the distribution of paragonimus species in Honghe Prefecture and Baoshan City of Yunnan Province, obtain pathogens from different hosts, and observe the morphology of metacercaria, post-cercariae, eggs and adults of paragonimus in both areas. The susceptibility of rats and domestic cats to paragonimus was compared, and the adults of paragonimus were observed by scanning electron microscopy (SEM). The species-genus relationship of paragonimus, the adaptability of host and the infection of the second intermediate host were determined in order to provide a useful scientific basis for the study and control of paragonimus in Yunnan Province. [methods] Creek crabs were collected from Jinping County and Baoshan City of Honghe Prefecture, Yunnan Province, respectively. After morphological observation and measurement, rats and domestic cats were infected by oral feeding, and the body and eggs were collected. The morphology of the adult was observed by scanning electron microscope (SEM), and the stained seal specimen was made from the worm body and demysted cercariae. [results] the morphology of cercariae in Jinping and Baoshan was similar, and they were single-layer cystic wall and round-like. The sizes were 0.401 mm 脳 0.395 mm and 0.435 mm 脳 0.433 mm., respectively. The adult was long ellipsoid, and the width-length ratios of 10.107 mm 脳 4.064mm and 9.594 mm 脳 3.577 mm, were 1? 2.49 and 1? 2.63, respectively. The ventral sucker was larger than the oral sucker, the ovary was close to the ventral sucker, and the lobulation was simple. Secondary lobulation is rare, testicular centrosome is small, its length is 1? 6 of the length of insect body. The skin spine of adult is mainly single-type, the skin spine in front of abdominal sucker is triangular or sharp knife-shaped, the end of skin spine after abdominal sucker is arc, a few appear bifurcation. The morphology of metacercaria, posterior cercariae, eggs and adults of paragonimus was similar, but the size of the first three was different, but there was no difference among adults. The infection rates of Jinping creek crab and Baoshan creek crab were 79. 9% and 100%, respectively. The average number of cysticercosis per crab and the average number of cysticercosis per gram of crab in Jinping creek were 1.8 and 0.7, respectively. The average number of cysticercosis and the average number of cysticercosis per gram of crab in Baoshan River crab were 13.3 and 2, respectively. The recovery rates were 24% and 55% for rats and domestic cats, 17% and 51.7% for adults, 5% and 61.7% for mice infected with cercariae from Baoshan, respectively, and 55% for mice infected with cercariae Jinping and 55% for cats, respectively, and the recovery rates for adults were 17% and 51.7%, respectively. The adult recovery rates were 4% and 51.7%. [conclusion] Jinping County and Baoshan City, Honghe Prefecture, Yunnan Province are the endemic areas of paragonimus, and the infection rate and infection degree of Baoshan Creek crabs are obviously higher than those of Jinping Creek Crab. The most prevalent paragonimus in Jinping County and Baoshan City is the same species of paragonimus Chen's paragonimus but there are morphological differences among different regions. The susceptibility of rats to paragonimus in Jinping was higher than that in Baoshan, and the susceptibility of domestic cats to paragonimus was similar. Compared with the final hosts of the two experiments, the susceptibility of the domestic cat was significantly higher than that of the rat, and the domestic cat was an ideal animal model of paragonimus Chen.
【學(xué)位授予單位】：昆明醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2007
【分類號】：R383.23

【引證文獻(xiàn)】

相關(guān)碩士學(xué)位論文 前1條

1 楊斌斌;云南省三地并殖吸蟲分子系統(tǒng)研究[D];昆明醫(yī)學(xué)院;2008年

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