【摘要】： 目的:研究層粘連蛋白(LN)對(duì)人絨毛膜促性腺激素(hCG)刺激下大鼠排卵前顆粒細(xì)胞孕酮(P)分泌的影響及可能的作用機(jī)制。 方法:分離未成熟大鼠排卵前顆粒細(xì)胞,分3組進(jìn)行培養(yǎng):LN鋪板組、未鋪板組及LN鋪板同時(shí)加入抗整合素α_6單抗(GoH3)組。觀察比較細(xì)胞貼壁的情況。血清培養(yǎng)48h后加入hCG,無(wú)血清條件下再培養(yǎng)48h后收集培養(yǎng)液作孕酮測(cè)定及LH/hCG受體分析。 結(jié)果: LN鋪板組顆粒細(xì)胞6小時(shí)即可貼壁及展開(kāi),而未鋪板組16h開(kāi)始貼壁及展開(kāi);無(wú)論hCG加入與否LN均可顯著刺激顆粒細(xì)胞孕酮的產(chǎn)生, 5ug/mL的GoH3能明顯抑制LN的此刺激作用。試驗(yàn)還觀察到LN使顆粒細(xì)胞LH/hCG受體親和力升高,受體數(shù)目上調(diào),增強(qiáng)hCG刺激下顆粒細(xì)胞的孕酮分泌。 結(jié)論: LN通過(guò)與細(xì)胞表面粘附分子整合素α_6β_1(integrinα_6β_1)相互作用,可能為卵巢內(nèi)的一種局部調(diào)節(jié)因素,干擾LH/hCG受體功能并增強(qiáng)顆粒細(xì)胞孕酮的產(chǎn)生,在圍排卵期顆粒細(xì)胞黃素化過(guò)程中發(fā)揮重要效應(yīng)。
[Abstract]:Aim: to study the effect of laminin (LN) on progesterone (P) secretion in rat granulosa cells stimulated by human chorionic gonadotropin (hCG) and its possible mechanism. Methods: pre-ovulatory granulosa cells were isolated from immature rats and cultured in three groups: LN group, LN group and anti-integrin 偽 _ 6 monoclonal antibody (GoH3) group. Observe and compare the cell adhesion. After 48 hours of serum culture, hCG, was added to the serum-free medium for 48 h and then collected for progesterone determination and LH/hCG receptor analysis. Results: the granulosa cells in the LN laminate group could adhere and expand in 6 hours, while those in the non-laminate group began to adhere and expand at 16 h. Whether hCG was added or not, LN could significantly stimulate progesterone production in granulosa cells, and GoH3 of 5ug/mL could significantly inhibit the stimulation of LN. It was also observed that LN increased the affinity of LH/hCG receptors and increased the number of receptors in granulosa cells, and enhanced the progesterone secretion of granulosa cells stimulated by hCG. Conclusion: through the interaction of LN with integrin 偽 _ 6 尾 _ 1 (integrin 偽 _ 6 尾 _ 1), it may be a local regulatory factor in ovary, interfere with the function of LH/hCG receptor and enhance the production of progesterone in granulosa cells. It plays an important role in the process of luteinization of granulosa cells during the peri-ovulatory period.
【學(xué)位授予單位】：華中科技大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2006
【分類號(hào)】：R33

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