【摘要】：背景與目的: 蛋白酶激活受體(Protease Activated Receptors,PARs)屬于G蛋白偶聯(lián)受體 家族成員,分布在多種細(xì)胞上。目前在人和小鼠共發(fā)現(xiàn)4種PARs,分別為PAR-1、 PAR-2、PAR-3和PAR-4。PARs可被多種蛋白酶激活,參與機(jī)體多種生理與病 理生理過程。目前,越來越多的研究顯示PARs在炎癥和變態(tài)反應(yīng)中起著重要的 作用。 嗜堿性粒細(xì)胞是一種重要的免疫細(xì)胞。細(xì)胞表面表達(dá)高親和力IgE受體 (FcεRI),并在相應(yīng)的過敏原作用下引起受體交聯(lián),導(dǎo)致細(xì)胞脫顆粒從而釋放多種 介質(zhì),包括組胺、IL-4、IL-13、白三烯、前列腺素E_2等,通過這些介質(zhì)直接作 用或者活化其它細(xì)胞從而參與炎癥和變態(tài)反應(yīng)等過程。 雖然PARs在炎癥與變態(tài)反應(yīng)中的作用已成為研究熱點(diǎn),但迄今為止,嗜堿 性粒細(xì)胞的PARs相關(guān)研究尚未見報(bào)道。4種PARs在嗜堿性粒細(xì)胞上的表達(dá)情 況如何,PARs對(duì)嗜堿性粒細(xì)胞的激活有無(wú)調(diào)節(jié)作用?以上種種問題尚待解答。 PARs對(duì)人嗜堿性粒細(xì)胞組胺釋放的影響的研究將有助于人們對(duì)PARs功能的了 解,并且對(duì)進(jìn)一步闡明嗜堿性粒細(xì)胞在變態(tài)反應(yīng)發(fā)生中的作用具有重要意義。 材料和方法: 用TRIZOL試劑提取人嗜堿性粒細(xì)胞系KU812總RNA,然后進(jìn)行RT-PCR
[Abstract]:Background & objective: protease-activated receptor (Protease Activated Receptors,PARs) is a member of the G-protein coupled receptor family and is distributed in a variety of cells. At present, four kinds of PARs, PAR-1, PAR-2,PAR-3 and PAR-4.PARs, were found to be activated by a variety of protease in human and mouse, respectively. Participate in a variety of physiological and pathological physiological processes. At present, more and more studies have shown that PARs plays an important role in inflammation and metamorphosis. Basophil is an important immune cell. High affinity IgE receptor (Fc 蔚 RI),) is expressed on the cell surface and is cross-linked with the corresponding allergen, resulting in the degranulation of cells and the release of a variety of mediators, including histamine. IL-4,IL-13, leukotriene, prostaglandin E _ 2 and so on, through these mediums are directly used or activated other cells to participate in inflammation and allergic reaction and other processes. Although the role of PARs in inflammation and hypersensitivity has become a hot topic, so far, PARs related studies on basophil have not been reported. How about the expression of four kinds of PARs on basophil? does PARs regulate the activation of basophil? The above questions remain unanswered. The study of the effect of PARs on the histamine release of human basophilic granulocytes will contribute to the understanding of the function of PARs, and it will be of great significance to further clarify the role of basophil in the pathogenesis of allergic reaction. Materials and methods: total RNA, of human basophilic granulocyte KU812 was extracted with TRIZOL reagent and then RT-PCR was performed.
【學(xué)位授予單位】：汕頭大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2005
【分類號(hào)】：R392

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