【摘要】：目的:①觀察胎兒海馬組織形態(tài)的發(fā)育變化。②觀察胎兒海馬內(nèi)神經(jīng)元的分化、生長及發(fā)育規(guī)律。③研究自分泌運(yùn)動因子(autocrine motility factor,AMF)及自分泌運(yùn)動因子受體(autocrine motility factor receptor,AMFR)在胎兒海馬中的表達(dá)和意義。④探討自分泌運(yùn)動因子及其受體與胎兒海馬發(fā)育的關(guān)系。方法:本實(shí)驗(yàn)收集胎兒29例,胎齡為11W～新生兒,根據(jù)海馬組織不同發(fā)育階段變化特點(diǎn),將胚胎標(biāo)本分三組,第一組為11W～16W,7例;第二組為17W～28W,17例;第三組為29W～新生兒,5例。取海馬中段,常規(guī)石蠟包埋和切片。分別進(jìn)行以下處理:①采用HE染色觀察胎兒海馬一般組織形態(tài)的發(fā)育變化;②采用硫堇染色觀察胎兒海馬神經(jīng)元內(nèi)尼氏體的發(fā)育變化;③應(yīng)用免疫組織化學(xué)SABC法檢測神經(jīng)元特異性烯醇化酶(neuron specific enolase,NSE)、AMF和AMFR的表達(dá)及變化規(guī)律。結(jié)果:①HE染色切片中,第11W～16W胎兒海馬的外部形態(tài)逐漸建立。海馬內(nèi)細(xì)胞分布密集,多為小而圓的細(xì)胞,胞質(zhì)少,染色深。第17W開始可明顯辨別海馬本部CA1、CA2、CA3及CA4各區(qū)及齒狀回,并可區(qū)分出海馬皮質(zhì)的分子層、錐體細(xì)胞層和多形細(xì)胞層。錐體細(xì)胞層內(nèi)可見少量錐體形細(xì)胞和大量圓形細(xì)胞。齒狀回可區(qū)分分子層、顆粒細(xì)胞層和多形細(xì)胞層,其中顆粒細(xì)胞層內(nèi)細(xì)胞多呈圓形或橢圓形。隨胎齡增長,海馬錐體細(xì)胞層內(nèi)細(xì)胞密度逐漸減小,細(xì)胞由圓形逐漸分化為錐體形,以中等大小的錐體細(xì)胞為多見。第29W以后,海馬錐體細(xì)胞層內(nèi)神經(jīng)元逐漸發(fā)育成熟,細(xì)胞體積進(jìn)一步增大,細(xì)胞密度進(jìn)一步減小。②硫堇染色結(jié)果顯示,在16W以前胎兒海馬內(nèi)幾乎看不到尼氏體;從17W開始,海馬錐體細(xì)胞層少數(shù)細(xì)胞內(nèi)出現(xiàn)尼氏體,隨胎齡增加,神經(jīng)元內(nèi)尼氏體漸趨豐富,染色加深。③免疫組織化學(xué)染色顯示,胎兒發(fā)育到第17W時,海馬的錐體細(xì)胞層和齒狀回的顆粒細(xì)胞層內(nèi)出現(xiàn)NSE陽性細(xì)胞,多形細(xì)胞層和分子層也有散在分布。NSE陽性細(xì)胞的分化速度隨層次和區(qū)域不同而呈現(xiàn)差異,在錐體
[Abstract]:Objective: 1 to observe the morphological changes of fetal hippocampal tissue, 2 to observe the differentiation, growth and development of fetal hippocampal neurons. 3 to study autocrine motor factor (autocrine motility factor,AMF) and autocrine motor factor receptor (autocrine motility factor receptor,). (4) to investigate the relationship between autocrine motor factor and its receptor and fetal hippocampal development. Methods: 29 fetuses (gestational age 11W ~ newborns) were collected and divided into three groups according to the changes of hippocampal tissue in different developmental stages: the first group was 11W ~ 16W (n = 7), the second group (n = 17) was 17W ~ 28W (n = 17), the second group (n = 17) was divided into three groups. The third group was 29 W ~ newborns, 5 cases. The middle part of hippocampus was taken and paraffin embedded and sectioned. The following treatments were carried out: (1) HE staining was used to observe the morphological changes of fetal hippocampal tissues, 2) thionine staining was used to observe the development of Nissl bodies in fetal hippocampal neurons. 3 the expression and changes of neuron-specific enolase (neuron specific enolase,NSE), AMF and AMFR were detected by immunohistochemical SABC method. Results: in the 1HE staining section, the outer shape of the hippocampus of the 11W~16W fetus was gradually established. Hippocampal cells are densely distributed, mostly small and round cells, with less cytoplasm and deep staining. At the beginning of 17W, the CA1,CA2,CA3 and CA4 regions and dentate gyrus of hippocampus could be distinguished, and the molecular layer, pyramidal cell layer and polymorphic cell layer of hippocampal cortex could be distinguished. A small number of pyramidal cells and a large number of round cells can be seen in the pyramidal cell layer. Dentate gyrus can distinguish molecular layer, granular cell layer and polymorphic cell layer, in which the cells in granular cell layer are round or oval. With the increase of gestational age, the density of cells in the pyramidal cell layer of hippocampus gradually decreased, and the cells gradually differentiated from round to pyramidal, most of which were medium size pyramidal cells. After 29W, neurons in the pyramidal layer of hippocampus matured gradually, cell volume further increased, cell density decreased further. 2 the results of thionine staining showed that almost no Nissl bodies could be seen in the hippocampus of the fetus before 16W. From 17W onwards, a few cells of hippocampal pyramidal cell layer appeared Nissl bodies. With the increase of gestational age, the neuronal Nissl bodies gradually enriched and the staining deepened. 3 Immunohistochemical staining showed that the fetus developed to 17W. NSE positive cells were found in the pyramidal cell layer and granular cell layer of dentate gyrus in the hippocampus, and scattered in the polymorphic cell layer and molecular layer. The differentiation rate of NSE positive cells varied with different levels and regions, and the differentiation rate was different in the pyramidal body.
【學(xué)位授予單位】：安徽醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2005
【分類號】：R329

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