【摘要】：目的： TAO是眼科臨床常見眼眶病病因之一，患病率在眼眶疾病中居首位。目前認(rèn)為，TAO是由于自身免疫反應(yīng)影響到眶周及球后組織，以淋巴細(xì)胞浸潤為特點(diǎn)伴有粘多糖及膠原沉積，以水腫、突眼和復(fù)視為臨床表現(xiàn)的一種器官特異性自身免疫性疾病。對TAO的研究要么是缺乏可復(fù)制的動物模型，要么就是在疾病的早期難以獲得眼眶組織，致其具體病因及確切的發(fā)病機(jī)制仍不完全明了。因此，要詳細(xì)了解TAO各方面的改變，探尋有效防治措施，就在于要建立適當(dāng)?shù)膭游锬Ｐ汀?目前國外多采用的TAO建模方法是用TSHR多肽或核酸免疫、TSHR轉(zhuǎn)染細(xì)胞或TSHR活化T細(xì)胞免疫和多基因聯(lián)合免疫等。比較理想的方法是TSHR活化細(xì)胞或TSHR基因免疫方法。 本研究旨在探討TSHR基因免疫方法構(gòu)建TAO動物模型，，觀察免疫后的TAO模型動物眼眶組織和甲狀腺的組織病理學(xué)變化以及眼眶組織Th1／Th2免疫反應(yīng)類型；同時研究IL-4與IFN-γ干預(yù)后TAO模型動物眼眶組織和甲狀腺組織病理學(xué)變化以及眼眶組織Th1／Th2免疫反應(yīng)平衡的改變，為進(jìn)一步探討TAO發(fā)病機(jī)制以及臨床防治奠定理論基礎(chǔ)。 方法： 本研究分為三個部分。
[Abstract]:Objective: TAO is one of the common causes of orbital diseases in ophthalmology. It is believed that TAO is an organ-specific autoimmune disease characterized by lymphocytic infiltration accompanied by mucopolysaccharide and collagen deposition, edema, exophthalmos and recombination as clinical manifestations due to the autoimmune effects of autoimmune responses to periorbital and retrobulbar tissues. The study of TAO is either lack of replicable animal models or it is difficult to obtain orbital tissue at the early stage of the disease. Therefore, it is necessary to establish appropriate animal models to understand the changes of TAO in detail and to explore effective prevention and treatment measures. At present, most TAO modeling methods are immunized with TSHR polypeptide or nucleic acid, TSHR transfected cells or TSHR activated T cell immunity and multi-gene combined immunity. The ideal method is TSHR activated cell or TSHR gene immunization. The purpose of this study was to investigate the establishment of TAO animal model by TSHR gene immunization and to observe the histopathological changes of orbital and thyroid tissues and the types of Th1/Th2 immunoreaction in orbital tissues of TAO model animals after immunization. At the same time, the pathological changes of orbital and thyroid tissues and the changes of Th1/Th2 immunoreaction balance in orbital tissues of TAO model rats after intervention with IL-4 and IFN- 緯 were studied, which laid a theoretical foundation for the further study of the pathogenesis of TAO and the clinical prevention and treatment. Methods: the study was divided into three parts.
【學(xué)位授予單位】：四川大學(xué)
【學(xué)位級別】：博士
【學(xué)位授予年份】：2006
【分類號】：R-332;R771.3

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