【摘要】：目的 通過該模型的建立，為新生兒興奮毒性腦損傷神經(jīng)保護(hù)的研究提供一種動(dòng)物模型，從而為新生兒圍生期腦損傷的臨床干預(yù)探索新的實(shí)驗(yàn)方法。 方法 選取不同窩別生后五天的ICR種小鼠60只，隨機(jī)分為實(shí)驗(yàn)組和對(duì)照組。實(shí)驗(yàn)組腦內(nèi)注射神經(jīng)興奮毒素ibotenate(鵝膏蕈氨酸)后再隨機(jī)分為A組和B組，對(duì)照組腦內(nèi)注射生理鹽水后也隨機(jī)分為C組和D組。腦內(nèi)注射后24h選取A、C組斷頭處死，，120h選取B、D組斷頭處死。取腦組織切片、染色后行組織學(xué)觀察，并通過有損傷的病理切片數(shù)量計(jì)算各組腦組織的損傷直徑。 結(jié)果 實(shí)驗(yàn)組平均腦損傷直徑明顯大于對(duì)照組，實(shí)驗(yàn)組平均腦損傷直徑與對(duì)照組相比較具有顯著的差異性(p＜0.01)，差別具有統(tǒng)計(jì)學(xué)意義。 結(jié)論 實(shí)驗(yàn)組與對(duì)照組相比除了機(jī)械性損傷外還能形成大腦皮層的萎縮和腦室周圍白質(zhì)區(qū)明顯的囊性病變。該模型的成功建立能為進(jìn)一步開展新生兒興奮毒性腦損傷神經(jīng)保護(hù)的研究奠定基礎(chǔ)。
[Abstract]:Objective to provide an animal model for the study of neuroprotection of neonatal excitotoxic brain injury, and to explore a new experimental method for the clinical intervention of neonatal perinatal brain injury. Methods 60 ICR mice were randomly divided into experimental group and control group. The experimental group was randomly divided into group A and group B after intracerebral injection of neuroexcitotoxin ibotenate, and the control group was also randomly divided into group C and group D after intracerebral injection of normal saline. 24 h after intracerebral injection, head-cut death was performed in group A C and group B D at 120 h. Brain tissue sections were taken and histologically observed after staining. The injury diameters of brain tissues were calculated by the number of pathological sections. Results the mean brain injury diameter in the experimental group was significantly larger than that in the control group, and there was significant difference between the experimental group and the control group (p < 0.01). Conclusion compared with the control group, the experimental group can cause atrophy of cerebral cortex and obvious cystic lesions in periventricular white matter area in addition to mechanical injury. The successful establishment of the model can lay a foundation for further research on neuroprotection of excitotoxic brain injury in neonates.
【學(xué)位授予單位】：昆明醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2006
【分類號(hào)】：R722.12;R-332

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