大鼠延髓巨細胞網(wǎng)狀核與腦神經(jīng)運動核的纖維聯(lián)系
發(fā)布時間：2018-12-20 22:29
【摘要】： 位于腦干網(wǎng)狀結構抑制區(qū)的巨細胞網(wǎng)狀核(NRG)是一個功能復雜的核團，其無論是對軀體運動，還是對內(nèi)臟活動都有影響。NRG對軀體運動的抑制性中樞調(diào)控作用，在神經(jīng)系統(tǒng)電生理學及形態(tài)學研究中已得到充分證明，但該核團與內(nèi)臟運動的聯(lián)系尚缺乏全面、系統(tǒng)的資料。本實驗擬從形態(tài)學上對巨細胞網(wǎng)狀核的功能聯(lián)系加以補充說明。 目的 1．觀察大鼠巨細胞網(wǎng)狀核與腦神經(jīng)運動核的直接纖維聯(lián)系。 2．比較兩種神經(jīng)示蹤劑WGA—HRP和DiI的標記效果。 方法 1．用微量注射法將碳素墨水注入一側巨細胞網(wǎng)狀核，進行尼氏染色，觀察NRG的定位準確性。 2．將WGA—HRP注入一側NRG，采用TMB—ST法成色，光學顯微鏡下觀察注射部位是否準確及標記情況。 3．將DiI注入一側NRG，取材，熒光顯微鏡下觀察注射部位及標記情況。 結果 1．一般軀體運動核：動眼神經(jīng)核、滑車神經(jīng)核雙側可見逆行標記細胞及順行神經(jīng)纖維終末，逆行標記細胞同側多于對側；展神經(jīng)核未見標記；舌下神經(jīng)核雙側也看到逆行標記細胞及神經(jīng)纖維終末。 2．一般內(nèi)臟運動核：下泌涎核、迷走神經(jīng)背核雙側均見少量逆行標記細胞及順行神經(jīng)纖維終末；上泌涎核未見標記。 3．特殊內(nèi)臟運動核：面神經(jīng)核、疑核、三叉神經(jīng)運動核雙側均發(fā)現(xiàn)逆行標記細胞及順行纖維末梢。 4．WGA—HRP和DiI標記的結果分布相似，不同的是，，DiI在下泌涎核未顯示出標記，且在其他核團標記的細胞數(shù)目沒有WGA—HRP標記的多。 結論 1．大鼠巨細胞網(wǎng)狀核與絕大多數(shù)腦神經(jīng)運動核有纖維聯(lián)系，且投射方向為雙向。 2．DiI在成年動物活體腦中的標記效果不及WGA—HRP理想。
[Abstract]:(NRG) in the reticular nucleus of giant cells located in the inhibitory region of the reticular structure of the brain stem is a complex nuclear mass, which affects both the somatic movement and the visceral activity. The inhibitory central regulation of NRG on the somatic movement. It has been fully proved in the electrophysiology and morphology of nervous system, but the connection between the nucleus and visceral movement is still lack of comprehensive and systematic data. In this study, the functional connections of the reticular nucleus of giant cells were elucidated morphologically. Objective 1. To observe the direct fibrous connection between the reticular nucleus of giant cells and the motor nucleus of the brain. 2. The labeling effects of WGA-HRP and DiI were compared. Methods 1. Carbon ink was injected into one side of giant cell reticular nucleus by microinjection, and the localization accuracy of NRG was observed. 2. 2. The injection of WGA-HRP into one side of NRG, was colored by TMB-ST method, and the accuracy and labeling of the injection site were observed under optical microscope. 3. DiI was injected into one side of NRG, and the injection site and labeling were observed under fluorescence microscope. Results 1.General somatomotor nucleus: retrograde labeled cells and anterograde nerve fiber terminals were found on both sides of oculomotor nucleus, trochlear nucleus, ipsilateral retrograde labeled cells were more than contralateral, abductor nucleus was not labeled. Retrograde labeled cells and nerve fiber terminals were also seen on both sides of hypoglossal nucleus. 2. In general visceral motor nucleus, a few retrograde labeled cells and antegrade nerve terminals were found in the lower salivary nucleus and the dorsal nucleus of vagus, but no labeling was found in the upper salivary nucleus. 3. Special visceral motor nuclei: retrograde labeled cells and antegrade fibrous endings were found in the facial nucleus, the nucleus suspect and the motor nucleus of the trigeminal nerve. The results of 4.WGA-HRP and DiI labeling were similar, the difference was that DiI showed no markers in the lower salivary nucleus, and the number of cells labeled in other nuclei was not more than that labeled by WGA-HRP. Conclusion 1. The reticular nucleus of giant cells in rats has fibrous connections with most of the motor nuclei of the brain, and the projection direction is bidirectional. 2. The labeling effect of 2.DiI in the living brain of adult animals was not as good as that of WGA-HRP.
【學位授予單位】：鄭州大學
【學位級別】：碩士
【學位授予年份】：2007
【分類號】：R322
本文編號：2388517
[Abstract]:(NRG) in the reticular nucleus of giant cells located in the inhibitory region of the reticular structure of the brain stem is a complex nuclear mass, which affects both the somatic movement and the visceral activity. The inhibitory central regulation of NRG on the somatic movement. It has been fully proved in the electrophysiology and morphology of nervous system, but the connection between the nucleus and visceral movement is still lack of comprehensive and systematic data. In this study, the functional connections of the reticular nucleus of giant cells were elucidated morphologically. Objective 1. To observe the direct fibrous connection between the reticular nucleus of giant cells and the motor nucleus of the brain. 2. The labeling effects of WGA-HRP and DiI were compared. Methods 1. Carbon ink was injected into one side of giant cell reticular nucleus by microinjection, and the localization accuracy of NRG was observed. 2. 2. The injection of WGA-HRP into one side of NRG, was colored by TMB-ST method, and the accuracy and labeling of the injection site were observed under optical microscope. 3. DiI was injected into one side of NRG, and the injection site and labeling were observed under fluorescence microscope. Results 1.General somatomotor nucleus: retrograde labeled cells and anterograde nerve fiber terminals were found on both sides of oculomotor nucleus, trochlear nucleus, ipsilateral retrograde labeled cells were more than contralateral, abductor nucleus was not labeled. Retrograde labeled cells and nerve fiber terminals were also seen on both sides of hypoglossal nucleus. 2. In general visceral motor nucleus, a few retrograde labeled cells and antegrade nerve terminals were found in the lower salivary nucleus and the dorsal nucleus of vagus, but no labeling was found in the upper salivary nucleus. 3. Special visceral motor nuclei: retrograde labeled cells and antegrade fibrous endings were found in the facial nucleus, the nucleus suspect and the motor nucleus of the trigeminal nerve. The results of 4.WGA-HRP and DiI labeling were similar, the difference was that DiI showed no markers in the lower salivary nucleus, and the number of cells labeled in other nuclei was not more than that labeled by WGA-HRP. Conclusion 1. The reticular nucleus of giant cells in rats has fibrous connections with most of the motor nuclei of the brain, and the projection direction is bidirectional. 2. The labeling effect of 2.DiI in the living brain of adult animals was not as good as that of WGA-HRP.
【學位授予單位】：鄭州大學
【學位級別】：碩士
【學位授予年份】：2007
【分類號】：R322
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