【摘要】：目的：探討內(nèi)毒素血癥大鼠血漿ET-1水平、腦底動脈內(nèi)皮素-1(Endothelin-1，ET-1)能神經(jīng)纖維誘發(fā)腦血管痙攣(Cerebral vasopasm，CVS)發(fā)生與發(fā)展的關(guān)系，為深入闡明內(nèi)毒素血癥誘發(fā)CVS的發(fā)病機制提供理論依據(jù)。方法：96只Wistar大鼠隨機分為正常對照組、內(nèi)毒素血癥組(3、6、12、24、48h)，分別用放射免疫分析(Radioimmunoassay，RIA)技術(shù)、免疫組織化學ABC法(Immunohistochemical technique ABC)對正常對照組、內(nèi)毒素血癥組(3、6、12、24、48h)血漿ET-1水平表達的變化及大鼠腦底動脈ET-1能神經(jīng)纖維進行觀察。結(jié)果：內(nèi)毒素血癥后3h、6h、12h大鼠血漿ET-1水平比正常對照組明顯升高(P＜0.05)，內(nèi)毒素血癥后24h和48h趨于正常(P＞0.05)；正常對照組大鼠大腦前動脈、大腦中動脈、大腦后動脈和基底動脈均可見棕褐色，呈細線狀ET-1能免疫反應(yīng)陽性纖維；內(nèi)毒素血癥后6h、12h組大鼠腦底動脈與正常對照組大鼠腦底動脈比較，ET-1能陽性神經(jīng)纖維密度明顯增多，尤其以內(nèi)毒素血癥后12h組大鼠腦底動脈ET-1能神經(jīng)纖維密度改變最為明顯，經(jīng)統(tǒng)計學檢驗(P＜0.05)，內(nèi)毒素血癥后48h組大鼠腦底動脈ET-1能神經(jīng)纖維密度無明顯變化(P＞0.05)。結(jié)論：內(nèi)毒素血癥大鼠血漿ET-1水平升高、腦底動脈ET-1能神經(jīng)纖維密度增加，提示ET-1參與了內(nèi)毒素血癥誘發(fā)腦血管痙攣的病理生理過程，表明ET-1在內(nèi)毒素血癥誘發(fā)腦血管痙攣中起重要作用，，揭示了腦血管痙攣的神經(jīng)-體液調(diào)節(jié)機制的新思路，同時為臨床預(yù)防和治療腦血
[Abstract]:Objective: to investigate the level of plasma ET-1 in rats with endotoxemia and the relationship between the occurrence and development of cerebral vasospasm (Cerebral vasopasm,CVS) induced by endothelin-1 (Endothelin-1,ET-1) nerve fibers in the basal cerebral artery. To provide a theoretical basis for elucidating the pathogenesis of CVS induced by endotoxemia. Methods: 96 Wistar rats were randomly divided into two groups: normal control group, endotoxemia group (36 / 24 h). Radioimmunoassay (Radioimmunoassay,RIA) technique and immunohistochemical ABC method (Immunohistochemical technique ABC) were used to treat normal control group and endotoxemia group (3 / 6), respectively. The changes of plasma ET-1 expression and ET-1 nerve fibers in rat cerebral artery were observed. Results: the level of plasma ET-1 in rats was significantly higher than that in the control group at 3h, 6h and 12h after endotoxemia (P < 0. 05), and tended to be normal at 24h and 48h after endotoxemia (P > 0. 05). In the control group, the anterior cerebral artery, middle cerebral artery, posterior cerebral artery and basilar artery were all brown, with thin line ET-1 immunoreactive fibers. Compared with the normal control group, the density of ET-1 positive nerve fibers increased significantly in the cerebral fundus artery of rats 6 h after endotoxemia and 12 h after endotoxemia. The density of ET-1 nerve fibers in the cerebral fundus artery of rats in 12h after endotoxemia was the most obvious (P < 0. 05). There was no significant change in the density of ET-1 nerve fibers in the cerebral fundus artery of rats 48 hours after endotoxemia (P > 0. 05). Conclusion: the level of plasma ET-1 and the density of ET-1 energy nerve fibers in the basal cerebral artery are increased in rats with endotoxemia, suggesting that ET-1 is involved in the pathophysiological process of cerebral vasospasm induced by endotoxemia. The results suggest that ET-1 plays an important role in vasospasm induced by endotoxemia. It also reveals a new idea of neurohumoral regulation in cerebral vasospasm, and it also provides a new approach for clinical prevention and treatment of cerebral blood.
【學位授予單位】：南昌大學
【學位級別】：碩士
【學位授予年份】：2006
【分類號】：R363

【參考文獻】

相關(guān)期刊論文 前3條

1 邢杰,王淑芬,翁福海;細胞因子與炎癥[J];天津醫(yī)科大學學報;2001年01期

2 林雪群,祝高春,戴育成,黃海鷹,熊建忠,石嘉慶;內(nèi)皮素在蛛網(wǎng)膜下腔出血誘發(fā)腦血管痙攣中的作用[J];中國臨床解剖學雜志;2004年04期

3 李鳴真,陸付耳;內(nèi)皮素與膿毒癥[J];中國危重病急救醫(yī)學;2000年06期

本文編號：2382814