【摘要】： 表皮生長因子受體(epidermal growth factor receptor, EGFR)的過度表達被認為是腫瘤細胞過度增殖的重要因素之一。抑制EGFR的過度表達可以有效抑制細胞的過度增殖,從而達到腫瘤臨床治療的目的。RNA干擾(RNA interference, RNAi)技術可以高效高特異性地抑制特異蛋白的表達量,在腫瘤的治療方面具有良好的應用前景。 本研究在體外培養(yǎng)角質形成細胞和舌鱗癌細胞的基礎上,根據(jù)小干擾RNA(small interfering RNA, siRNA)的設計原則設計并合成以EGFR為靶點的siRNA,通過RNAi技術研究其對角質形成細胞和舌鱗癌細胞EGFR mRNA表達的抑制作用。從實驗結果中篩選出一條高效的以EGFR為靶點siRNA。研究其對舌鱗癌細胞增殖的抑制作用。該研究為以EGFR為靶點的siRNA臨床治療鱗癌、銀屑病等EGFR過度表達的臨床疾病研究提供一種新的實驗方法,為以RGFR為靶點藥物在臨床疾病治療中的應用提供一定的實驗依據(jù)。
[Abstract]:The overexpression of epidermal growth factor receptor (epidermal growth factor receptor, EGFR) is considered to be one of the important factors in the proliferation of tumor cells. Inhibiting the overexpression of EGFR can effectively inhibit the excessive proliferation of cells and achieve the purpose of clinical treatment of tumor. RNA interference (RNA interference, RNAi) technique can inhibit the expression of specific protein efficiently and specifically. It has a good prospect in the treatment of tumor. In this study, keratinocytes and tongue squamous carcinoma cells were cultured in vitro, and siRNA, targeting EGFR was designed and synthesized according to the design principle of small interference RNA (small interfering RNA, siRNA). The inhibition of EGFR mRNA expression in keratinocytes and tongue squamous carcinoma cells was studied by RNAi technique. A highly efficient siRNA. targeting EGFR was selected from the experimental results. To study its inhibitory effect on the proliferation of tongue squamous cell carcinoma cells. This study provides a new experimental method for the clinical treatment of squamous cell carcinoma, psoriasis and other overexpressed EGFR diseases with siRNA targeted at EGFR, and provides a certain experimental basis for the application of RGFR as a target drug in the treatment of clinical diseases.
【學位授予單位】：吉林大學
【學位級別】：碩士
【學位授予年份】：2007
【分類號】：R346

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本文編號：2377892