酶聯(lián)免疫S-HpAg快速診斷試劑盒的制備
發(fā)布時間：2018-12-13 00:29
【摘要】：1982年澳大利亞學(xué)者Warran和Marshall從慢性胃炎患者胃粘膜中培養(yǎng)出幽門螺桿菌(Helicobacter pylori,H.pylori)以來,H.pylori受到了極大的關(guān)注。H.pylori是一種傳染率高的有害菌,全世界約有50%人口被其感染。H.pylori與胃癌(gastric carcinoma,GC)及胃MALT(Mucosa Association Lymphoid Tissue)惡性淋巴瘤的發(fā)生關(guān)系密切。此外,H.pylori還可引起其它一些疾病,如冠心病,兒童生長遲緩或消化不良等。WHO和國際抗癌聯(lián)盟已將其列為1類致癌因子。 目前,診斷幽門螺桿菌感染的方法從檢測手段上分為侵入性和非侵入性兩大類。侵入性診斷方法最大的缺點,或者說受到的限制是必需通過胃鏡取樣本,造成胃病患者額外增加一些損傷與痛苦。非侵入性診斷方法也有缺陷,如放射性同位素本身會污染環(huán)境,需用氣體同位素質(zhì)譜儀測定,以及受抗體回憶反應(yīng)影響造成假陰(陽)性結(jié)果。 S—HpAg檢測作為一種新的HP檢測方法,具有檢測快速、經(jīng)濟(jì)、準(zhǔn)確、宜于大規(guī)模普查等優(yōu)點,且不受抗體回憶反應(yīng)影響,結(jié)果穩(wěn)定、特異性高,可用于HP的臨床診斷、根除療效觀察和隨訪復(fù)查等,也適合于兒童及孕婦檢測。 本實驗建立了4株穩(wěn)定分泌抗人HP單克隆抗體細(xì)胞株:E4、B7、G2、G3.完成了單克隆抗體的篩選、制備、純化、鑒定、工作單抗配對等方面的工作,為進(jìn)一步進(jìn)行臨床試驗打下了基礎(chǔ)。同時,HP的酶聯(lián)免疫試劑盒還為膠體金試劑盒的研制作出了重要的前期工作,給后者打下了必要的基礎(chǔ)。已申請一項國家發(fā)明專利,申請?zhí)枮?005102002286。
[Abstract]:Since Australian scholars Warran and Marshall cultured Helicobacter pylori (Helicobacter pylori,H.pylori) from gastric mucosa of patients with chronic gastritis in 1982, H.pylori has received great attention. H.pylori is a harmful bacterium with high infection rate. About 50% of the world's population is infected with H.pylori, which is closely related to the occurrence of gastric cancer (gastric carcinoma,GC) and gastric MALT (Mucosa Association Lymphoid Tissue) malignant lymphoma. In addition, H.pylori can also cause other diseases, such as coronary heart disease, growth retardation in children or indigestion, etc. WHO and the International Union against Cancer have classified it as a carcinogen of class 1. At present, the diagnostic methods of Helicobacter pylori infection can be divided into invasive and non invasive methods. The greatest drawback of invasive diagnosis, or limitation, is the need for gastroscopy to cause additional damage and pain in patients with gastropathy. Noninvasive diagnostic methods are also flawed, such as radioisotopes themselves that pollute the environment, gas isotope mass spectrometers and pseudo (positive) results due to the effect of antibody recall reactions. As a new method of HP detection, S-HpAg detection has the advantages of rapid, economical, accurate, suitable for large-scale screening, and can be used in the clinical diagnosis of HP without the influence of antibody recall reaction. The observation of eradication effect and follow-up examination are also suitable for children and pregnant women. In this study, we established four stable secreting monoclonal antibody cell lines against human HP: E4B7G2G3. The selection, preparation, purification, identification and matching of monoclonal antibodies were completed, which laid a foundation for further clinical trials. At the same time, the enzyme linked immunosorbent kit (Elisa) of HP has also made an important preliminary work for the development of colloidal gold kit and laid a necessary foundation for the latter. Has applied for a national invention patent, the application number is 200510 2002286.
【學(xué)位授予單位】：蘭州大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2006
【分類號】：R392
[Abstract]:Since Australian scholars Warran and Marshall cultured Helicobacter pylori (Helicobacter pylori,H.pylori) from gastric mucosa of patients with chronic gastritis in 1982, H.pylori has received great attention. H.pylori is a harmful bacterium with high infection rate. About 50% of the world's population is infected with H.pylori, which is closely related to the occurrence of gastric cancer (gastric carcinoma,GC) and gastric MALT (Mucosa Association Lymphoid Tissue) malignant lymphoma. In addition, H.pylori can also cause other diseases, such as coronary heart disease, growth retardation in children or indigestion, etc. WHO and the International Union against Cancer have classified it as a carcinogen of class 1. At present, the diagnostic methods of Helicobacter pylori infection can be divided into invasive and non invasive methods. The greatest drawback of invasive diagnosis, or limitation, is the need for gastroscopy to cause additional damage and pain in patients with gastropathy. Noninvasive diagnostic methods are also flawed, such as radioisotopes themselves that pollute the environment, gas isotope mass spectrometers and pseudo (positive) results due to the effect of antibody recall reactions. As a new method of HP detection, S-HpAg detection has the advantages of rapid, economical, accurate, suitable for large-scale screening, and can be used in the clinical diagnosis of HP without the influence of antibody recall reaction. The observation of eradication effect and follow-up examination are also suitable for children and pregnant women. In this study, we established four stable secreting monoclonal antibody cell lines against human HP: E4B7G2G3. The selection, preparation, purification, identification and matching of monoclonal antibodies were completed, which laid a foundation for further clinical trials. At the same time, the enzyme linked immunosorbent kit (Elisa) of HP has also made an important preliminary work for the development of colloidal gold kit and laid a necessary foundation for the latter. Has applied for a national invention patent, the application number is 200510 2002286.
【學(xué)位授予單位】：蘭州大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2006
【分類號】：R392
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相關(guān)期刊論文 前10條
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