【摘要】：目的 本實驗是在先前發(fā)現(xiàn)甘氨酸(Glycine)有心臟保護作用及在心肌細胞上發(fā)現(xiàn)有甘氨酸受體的基礎(chǔ)上，進一步從自由基代謝、細胞內(nèi)鈣超載及細胞凋亡等方面探討甘氨酸受體在心臟保護中的作用，，并用甘氨酸受體阻斷法和消除細胞外氯離子法證明甘氨酸受體的性質(zhì)。 方法 1、利用原代培養(yǎng)的SD大鼠乳鼠心肌細胞建立心肌缺氧／復(fù)氧(anoxia／reoxygenation，A／R)模型，實驗分為9組：(1)對照組；(2)甘氨酸組；(3)士的寧組；(4)消除細胞外氯離子+甘氨酸組；(5)A／R組；(6)(甘氨酸+A／R)組；(7)(士的寧+A／R)組；(8)(甘氨酸+士的寧+A／R)組；(9)(消除細胞外氯離子+甘氨酸+A／R)組。測定各組培養(yǎng)心肌細胞內(nèi)超氧化物歧化酶(SOD)、丙二醛(MDA)、一氧化氮(NO)的含量。 2、利用原代培養(yǎng)的SD大鼠乳鼠心肌細胞建立心肌缺氧／復(fù)氧(anoxia/reoxygenation，A／R)模型，實驗分組同上。測定心肌細胞存活率，各組用Fluo—3／AM染色測鈣和Annexin V-FITC／PI雙染色測細胞凋亡率，流式細胞儀檢測。 結(jié)果 (1)A／R組SOD、NO含量低于對照組(P＜0.05)，而MDA含量高于對照組。A／R組用甘氨酸處理后SOD、NO含量升高，MDA含量降低，與A／R組比較差異有統(tǒng)計學(xué)意義。而A／R組用甘氨酸+士的寧處理后，甘氨酸的上述保護作用被士的寧阻斷，與A／R組比較差異無統(tǒng)計學(xué)意義。消除細胞外氯離子+甘氨酸+A／R時，甘氨酸的保護作用也被阻斷，與A／R組比較差異無統(tǒng)計學(xué)意義。 (2)A／R組心肌細胞內(nèi)游離鈣的濃度和細胞凋亡率高于對照組(P＜0.05)。A／R組用甘氨酸處理后心肌細胞內(nèi)游離鈣的濃度和細胞凋亡率降低，與A／R組
[Abstract]:Objective to study the effects of glycine (Glycine) on cardiac protection and glycine receptor in cardiomyocytes. The role of glycine receptor in cardiac protection was investigated in the aspects of intracellular calcium overload and apoptosis. The properties of glycine receptor were proved by blocking glycine receptor and eliminating extracellular chloride ion. Methods 1. Myocardial hypoxia / reoxygenation (anoxia/reoxygenation,A/R) model was established by primary cultured neonatal SD rat cardiomyocytes. The model was divided into 9 groups: (1) control group, (2) glycine group, and (2) glycine group. (3) strychnine group; (4) elimination of extracellular chloride ion glycine group; (5) A / R group; (6) glycine A / R group; (7) strychnine A / R group; (8) glycine strychnine A / R group; (9) (elimination of extracellular chloride ion glycine A / R) group. The content of superoxide dismutase (SOD),) malondialdehyde (MDA),) nitric oxide (NO) in cultured cardiomyocytes was measured. 2Myocardial hypoxia / reoxygenation (anoxia/reoxygenation,A/R) model was established by primary cultured neonatal SD rat cardiomyocytes. The survival rate of cardiomyocytes was measured. The apoptosis rate was measured by Fluo-3/AM staining and Annexin V-FITC/PI double staining. Flow cytometry was used to detect the apoptosis rate. Results (1) the content of SOD,NO in group A / R was lower than that in control group (P < 0. 05), while the content of MDA in group A / R was higher than that in control group. The content of SOD,NO increased and the content of MDA decreased after treatment with glycine in group A / R (P < 0. 05). The difference was statistically significant compared with the A / R group. The protective effect of glycine was blocked by strychnine in group A / R, and there was no significant difference between group A / R and group A / R. The protective effect of glycine was also blocked when the extracellular chloride ion glycine A / R was eliminated, and there was no significant difference compared with the A / R group. (2) the concentration of intracellular free calcium and the rate of apoptosis in the group A / R were higher than those in the control group (P < 0.05), the concentration of free calcium and the rate of apoptosis in the group of A / R were lower than those in the group of A / R (P < 0.05).
【學(xué)位授予單位】：暨南大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2006
【分類號】：R363

【參考文獻】

相關(guān)期刊論文 前10條

1 張榮慶,程何祥,馬穎艷,徐凱,賈國良,李飛,劉兵;粉防己堿對新生大鼠心肌細胞低氧/復(fù)氧損傷中細胞凋亡的影響[J];第四軍醫(yī)大學(xué)學(xué)報;2003年04期

2 李俊峽,賈國良,金明,馬越云,蘇明權(quán);轉(zhuǎn)染bcl-2基因?qū)π募〖毎脱?復(fù)氧損傷中細胞凋亡的影響[J];第四軍醫(yī)大學(xué)學(xué)報;2004年09期

3 許秀芳,李溫斌,陳寶田,呂燕寧,趙莉敏,陳燕;成年大鼠心肌細胞培養(yǎng)方法的建立和形態(tài)學(xué)觀察[J];首都醫(yī)科大學(xué)學(xué)報;2000年02期

4 張北奇，馬寧，董力，周錫英，于占久，伍貽經(jīng);培養(yǎng)大鼠心肌細胞缺氧與復(fù)氧時H~＋－Ca~（2＋）交換的研究[J];生理學(xué)報;1995年01期

5 曹云新,劉智廣,張曉楠;流式細胞術(shù)細胞周期分析樣品保存條件的研究[J];細胞與分子免疫學(xué)雜志;1999年04期

6 李俊峽,賈國良,李佃貴,李振彬,虞國杰,蔡春林;心肌缺血/再灌注時心肌細胞凋亡與心功能關(guān)系的實驗研究[J];心臟雜志;2004年01期

7 戚仁斌,陸大祥,王華東,周華,王海華,李楚杰;甘氨酸拮抗內(nèi)毒素性離體心臟損傷的作用研究[J];中國藥理學(xué)通報;2005年06期

8 丁勇,陸大祥,孫崴,張穗梅,李楚杰;甘氨酸對內(nèi)毒素誘導(dǎo)單核細胞產(chǎn)生IL-1和TNF的影響[J];中國藥理學(xué)通報;1999年02期

9 王嘉寧;郭寧;;細胞凋亡的檢測技術(shù)與方法[J];中國藥理學(xué)與毒理學(xué)雜志;2005年06期

10 ;甘氨酸對內(nèi)毒素所致心肌損傷的影響[J];中國病理生理雜志;2001年08期

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