【摘要】：目的：研究自行設(shè)計(jì)的高蔗糖飼料喂養(yǎng)SD大鼠制備正常體重胰島素抵抗(IR)大鼠模型，并評(píng)價(jià)該飲食對(duì)大鼠IR的影響及可能機(jī)制。 方法：30只雄性SD大鼠隨機(jī)分為兩組：正常對(duì)照(normal control，NC)組(n=15)，喂養(yǎng)普通飲食；試驗(yàn)組即高蔗糖(high-sucrose，HS)組，喂養(yǎng)高蔗糖飲食。實(shí)驗(yàn)期間每組給予相應(yīng)的飼料喂養(yǎng)，每3天測(cè)量體重，喂養(yǎng)16周后行腹腔內(nèi)注射葡萄糖耐量試驗(yàn)(IPGTT)。喂養(yǎng)20周后采空腹血樣測(cè)量空腹血糖(FBG)、總膽固醇(TC)、甘油三脂(TG)、高密度脂蛋白(HDL-c)、游離脂肪酸(FFA)及胰島素水平。并采用3-~3H標(biāo)記葡萄糖作為示蹤劑建立自由狀態(tài)下大鼠擴(kuò)展高胰島素-正常血糖鉗夾技術(shù)評(píng)價(jià)動(dòng)物胰島素敏感性。測(cè)定腹內(nèi)脂肪重量。取肝臟、骨骼肌、胸主動(dòng)脈做HE染色、肝臟蘇丹Ⅲ染色光鏡及電鏡觀察大鼠組織形態(tài)學(xué)變化。 結(jié)果：HS組與NC組大鼠比較，體重?zé)o明顯差異，HS組糖耐量減低，HS組TG、空腹胰島素水平升高，，HDL-c下降，兩組FBG、TC、FFA無明顯差異。HS組鉗夾穩(wěn)態(tài)GIR明顯降低，HS組GDR明顯下降，HGO增加。表明本高蔗糖飲食可成功誘導(dǎo)大鼠產(chǎn)生外周及肝臟的IR。HS組腹內(nèi)脂肪較NC組明顯增多。將所有大鼠鉗夾時(shí)的GDR與腹內(nèi)脂肪重量作相關(guān)性分析，結(jié)果提示GDR與腹內(nèi)脂肪重量呈負(fù)相關(guān)。病理觀察，HS組大鼠肝臟
[Abstract]:Aim: to study the effect of high sucrose diet on insulin resistance (IR) rat model of normal body weight in SD rats, and to evaluate the effect of high sucrose diet on IR in rats and its possible mechanism. Methods: thirty male SD rats were randomly divided into two groups: normal control (normal control,NC) group (n = 15) and high sucrose (high-sucrose,HS) group. During the course of the experiment, each group was fed with corresponding feed. Body weight was measured every 3 days. After 16 weeks of feeding, intraperitoneal injection of glucose tolerance test (IPGTT).) was performed. Fasting blood samples were collected after 20 weeks of feeding. Fasting blood glucose (FBG), total cholesterol (TC), triglyceride (TG), high density lipoprotein (HDL-c), free fatty acid (FFA) and insulin were measured. In addition, 3-tri-3H labeled glucose was used as tracer to establish the expanded hyperinsulin-normal glucose clamp technique in free state to evaluate the insulin sensitivity of animals. The weight of abdominal fat was measured. The liver, skeletal muscle and thoracic aorta were stained with HE. The histological changes of rats were observed under light microscope and electron microscope. Results: there was no significant difference in body weight between HS group and NC group. In HS group, glucose tolerance was decreased, fasting insulin level of TG, in HS group was increased, HDL-c level was decreased, and FBG,TC,FFA was not significantly different between the two groups. GDR decreased and HGO increased in HS group. The results showed that the high sucrose diet could induce the abdominal fat of the IR.HS group which produced peripheral and liver were significantly higher than that of the NC group. The correlation analysis between GDR and abdominal fat weight in all rats showed that GDR was negatively correlated with abdominal fat weight. Pathological observation: HS group rats live
【學(xué)位授予單位】：四川大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2006
【分類號(hào)】：R-332;R587.1

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1 劉雪輝;體重正常胰島素抵抗大鼠模型制備與評(píng)價(jià)[D];四川大學(xué);2006年

2 浦陽;肥胖與正常體重大學(xué)生的體力活動(dòng)與體質(zhì)研究[D];大連理工大學(xué);2008年

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