發(fā)布時間：2018-11-16 07:44

【摘要】：目的：觀察大鼠肝缺血再灌注(I／R)損傷過程對腎的影響并探討其可能的機(jī)制。方法：建立肝缺血再灌注損傷動物模型，選健康Wistar大鼠48只，雌雄各半，按阻斷血流后不同時間點(diǎn)分為六組，即對照組、缺血30min組(I組)、缺血30min再灌注組(I／R組)、缺血30min再灌注后1h組(I／R1h組)、缺血30min再灌注后2h組(I／R2h組)、缺血30min再灌注后4h組(I／R4h組)，每組8只。每組均測定血中谷氨酰轉(zhuǎn)肽酶(GTP)、血清磷酸酶(AKP)、γ-谷氨酰氨基轉(zhuǎn)移酶(γ-GT)、尿素氮(BUN)、肌酐(Cr)及腎組織中的丙二醛(MDA)的含量，并測定血漿和腎組織血管緊張素Ⅱ(AngⅡ)、醛固酮(Ald)、內(nèi)皮素(ET)的含量。結(jié)果：肝臟I／R導(dǎo)致肝臟明顯的損傷，表現(xiàn)為血清GTP在I組與對照組相比明顯升高，差異有統(tǒng)計學(xué)意義(P＜0.05)；I／R、I／R1h、I／R2h、I／R4h組與I組比較，差異有統(tǒng)計學(xué)意義(P＜0.05)；I／R1h、I／R2h、I／R4h組與I／R組比較，差異有統(tǒng)計學(xué)意義(P＜0.05)；I／R2h、I／R4h組與I／R1h組比較差異有統(tǒng)計學(xué)意義(P＜0.05)；I／R4h組與I／R2h組相比，差異有統(tǒng)計學(xué)意義(P＜0.05)。AKP在I組與對照組相比明顯升高，差異有統(tǒng)計學(xué)意義(P＜0.05)；I／R、I／R1h、I／R2h、I／R4h組與I組比較，，差異有統(tǒng)計學(xué)意義(P＜0.05)。γ-GT的含量也有升高的趨勢，I／R1h、I／R2h、I／R4h組與對照組、I、I／R組比較，差異有統(tǒng)計學(xué)意義(P＜0.05)。隨著肝I／R時間的延長，肝臟結(jié)構(gòu)出現(xiàn)了明顯改變，表現(xiàn)為炎性細(xì)胞浸潤，肝細(xì)胞片狀壞死。血中BUN含量隨再灌注時間延長逐漸升高，I／R2h、I／R4h組與對照組、I、I／R、I／R1h組相比明顯升高，差異有統(tǒng)計學(xué)意義(P＜0.05)；I／R4h組與I／R2h組相比，差異有統(tǒng)計學(xué)意義(P＜0.05)。血中Cr以及腎組
[Abstract]:Aim: to observe the effect of hepatic ischemia reperfusion (I / R) injury on the kidney and to explore its possible mechanism. Methods: the animal model of hepatic ischemia-reperfusion injury was established. 48 healthy Wistar rats, half male and half female, were divided into six groups according to different time points after occlusion: control group, ischemic 30min group (group I) and ischemic 30min reperfusion group (I / R group). One hour after 30min reperfusion (I/R1h group), two hours after ischemia 30min reperfusion (I/R2h group) and four hours after ischemia 30min reperfusion (I/R4h group), 8 rats in each group. The levels of serum (AKP), 緯 -glutamyl aminotransferase (AKP), 緯 -glutamyl aminotransferase) (AKP), 緯 -glutamyl aminotransferase) in serum and malondialdehyde (MDA) in renal tissue were measured in each group. The contents of angiotensin 鈪

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