【摘要】：目的:探討不同劑量鏈脲佐菌素(STZ)誘導(dǎo)小鼠糖尿病模型的機制,尋找STZ 誘導(dǎo)1 型糖尿病模型的最佳劑量。方法:本研究采用多次重復(fù)注射不同劑量(20mg/kg·bw,40mg/kg·bw,80mg/kg·bw)的STZ 誘導(dǎo)昆明小鼠糖尿病模型;采用葡萄糖(Glu)測定試劑盒與尿液分析試紙條聯(lián)合檢測了小鼠血糖和尿糖的變化;采用常規(guī)HE 切片光鏡下觀察的方法檢測胰島的組織學(xué)改變;采用酶聯(lián)免疫吸附試驗(間接ELISA 法)測定小鼠血清中胰島素自身抗體(IAA)的水平;采用淋巴細胞轉(zhuǎn)化試驗(MTT 法)測定小鼠胸腺和脾臟的T 淋巴細胞功能。結(jié)果:1.STZ 劑量選擇:本研究應(yīng)用20mg/kg·bw,40mg/kg·bw,80mg/kg·bw 多次注射STZ 誘導(dǎo)小鼠DM。2.體重:在注射STZ 后第三天Ⅳ組小鼠的體重明顯下降,而Ⅱ組、Ⅲ組也較對照組輕度下降。隨著時間的推移,Ⅳ組小鼠的體重不斷下降,而Ⅱ組、Ⅲ組則隨時間增長而體重增加,但與對照組相比Ⅱ組、Ⅲ組小鼠體重的增加是緩慢的。3. 血糖、尿糖:在最后一次注射STZ 后短期內(nèi)(第4 天)Ⅳ組模型鼠的血糖即升高,而在第14 天～21 天血糖值開始下降;尿糖均++++。Ⅱ組、Ⅲ組模型鼠血糖雖在開始升高不明顯,但1 周后的血糖值則隨時間增長逐漸持續(xù)增加;尿糖Ⅱ組中有5 只為-,其余均+,Ⅲ組中有2 只為-,且均為雌性,其余均++。Ⅰ組小鼠血糖值隨時間增長沒有變化,尿糖均為-。4.胰腺組織學(xué)觀察:正常組的胰島為圓形、橢圓形或條索狀細胞團,β細胞數(shù)量較多,胞質(zhì)豐富,核圓形。Ⅱ組、Ⅲ組成模鼠的胰島變小,胰島細胞數(shù)量減少,可見以淋巴細胞、單核細胞為主體的炎細胞浸潤,胰島β細胞空泡變性、壞死、消失,使胰島呈空虛狀態(tài);Ⅳ組小鼠偶見胰島存在。5.自身抗體產(chǎn)生:在注射STZ 后短期內(nèi)(第4 天)各組與對照組無明顯差別;但隨著時間的推移(第7 天起)Ⅱ組和Ⅲ組的自身抗體增加,而Ⅳ
[Abstract]:Aim: to investigate the mechanism of different doses of streptozotocin (STZ) induced diabetic model in mice and to find out the best dose of STZ induced type 1 diabetes. Methods: Kunming mice model of diabetes was induced by multiple repeated injection of different doses (20mg/kg bw,40mg/kg bw,80mg/kg bw) of STZ. Glucose (Glu) assay kit and urine analysis strip were used to detect the changes of blood glucose and urine sugar in mice, and the histological changes of pancreatic islets were detected under light microscope with routine HE sections. Enzyme linked immunosorbent assay (ELISA) was used to detect the level of insulin autoantibodies (IAA) in serum of mice, and lymphocyte transformation test (MTT) was used to detect the function of T lymphocytes in thymus and spleen of mice. Results: 1.STZ dose selection: in this study, mice DM.2. was induced by multiple injection of STZ with 20mg/kg bw,40mg/kg bw,80mg/kg bw. Body weight: on the third day after STZ injection, the body weight of group 鈪，

本文編號：2334767