【摘要】： 本研究首先依據(jù)中醫(yī)關(guān)于肝郁脾虛證的發(fā)病學(xué)說、臨床表現(xiàn)和其涉及到某些現(xiàn)代疾病的生病理變化特點(diǎn),探索運(yùn)用復(fù)合病因造模法復(fù)制出肝郁脾虛證大鼠模型,通過神經(jīng)-內(nèi)分泌-免疫系統(tǒng)中的多指標(biāo)觀察,以獲得模型的中醫(yī)證候及部分生物學(xué)特點(diǎn);在此基礎(chǔ)上,比較觀察具有疏肝、健脾和疏肝健脾功用的柴胡疏肝散、四君子湯和柴疏四君湯三方對該肝郁脾虛證模型的作用異同,探討異方同證條件下的三方效用與病證模型之間的關(guān)聯(lián)性和關(guān)聯(lián)的部分現(xiàn)代生物學(xué)基礎(chǔ)。故本課題以研究不同方劑與同一對應(yīng)主治證之間的關(guān)系為切入點(diǎn),為進(jìn)一步揭示和探索“方證相關(guān)”這一邏輯命題提供實證依據(jù)。論文分為文獻(xiàn)綜述和實驗研究兩部分。文獻(xiàn)綜述部分主要對肝郁脾虛證的臨床與實驗研究進(jìn)展、柴胡疏肝散、四君子湯及柴疏四君湯的臨床與實驗研究進(jìn)展進(jìn)行了綜述。實驗研究部分主要從肝郁脾虛證模型的神經(jīng)-內(nèi)分泌-免疫網(wǎng)絡(luò)、胃腸功能的變化、柴胡疏肝散、四君子湯及柴疏四君湯對模型神經(jīng)-內(nèi)分泌-免疫網(wǎng)絡(luò)、胃腸功能變化的影響及柴疏四君湯對模型甲狀腺軸、能量代謝、血循環(huán)變化的調(diào)節(jié)作用三方面進(jìn)行了探討。 研究一:肝郁脾虛證模型的建立 方法:將大鼠隨機(jī)分為正常組和肝郁脾虛模型組,每組40只。模型組大鼠采用慢性束縛應(yīng)激+過度疲勞+飲食失節(jié)法造模,將大鼠于每天上午8∶00置于束縛盒中限制3小時,下午2∶00置于盛有溫水(22±1℃)的大塑料桶中游泳10分鐘。隔日喂食(隔日禁食,隔日足量給食),連續(xù)3周。然后停止造模自然恢復(fù)一周。分別于造模的第一、二、三、四周取材,用鄰-甲苯胺法測尿D-木糖排泄率,用高效液相色譜-電化學(xué)法檢測單胺神經(jīng)遞質(zhì),用試劑盒放免法檢測血清GAS、IL-1β、IL-2、IL-6、TNF;血漿MTL、SS、VIP、ACTH、CORT;下丘腦CRH的含量。 結(jié)果: 1.外觀行為的變化:隨著造模天數(shù)的增加,肝郁脾虛組大鼠毛發(fā)不澤、枯亂;少動、扎堆、不活躍;情緒低落;飲食減少;易醒、倦臥;便溏;消瘦。停止造模一周后各種變化未見明顯恢復(fù)。體重顯著降低。 2.胃腸功能、神經(jīng)遞質(zhì):模型組大鼠尿D-木糖排泄率顯著降低;血清GAS第三和第四周顯著高于正常組;血漿SS第二周顯著低于正常組,第三、四周顯著高于正常組;血漿MTL第一、四周顯著高于正常組,第二、三周顯著低于正常組;血漿VIP第三周顯著低于正常組,第四周顯著高于正常組。模型組大鼠海馬NE第三和四周顯著低于正常組;海馬DA與正常組無明顯差異;海馬5-HT第二周顯著降低,第三和四周顯著升高。 3.免疫系統(tǒng):模型組大鼠胸腺、脾重量在造模第一至第四周顯著降低;胸腺指數(shù)在第一、二周顯著降低,至第三、四周無顯著差異,脾指數(shù)于造模第三周和第四周時顯著
[Abstract]:According to the theory of the pathogenesis of liver stagnation and spleen deficiency syndrome, the clinical manifestations and the characteristics of pathophysiological changes related to some modern diseases, this study first explored the use of compound etiology to make a model of liver stagnation and spleen deficiency syndrome in rats. In order to obtain the TCM syndromes and some biological characteristics of the model by observing the multiple indexes in the neuro-endocrine-immune system; On this basis, the effects of Chaihu Shugan San, Sijunzi decoction and Chaisusijun decoction, which have the functions of soothing liver, strengthening spleen and soothing liver and strengthening spleen, on the model of liver-stagnation and spleen deficiency were compared and observed. To explore the relationship between tripartite utility and disease syndrome model and some modern biological basis of correlation under the condition of syndromes of different prescription and syndromes. Therefore, the research on the relationship between different prescriptions and the same corresponding main treatment syndrome is the breakthrough point, which provides an empirical basis for further revealing and exploring the logical proposition of "the correlation of prescription and syndrome". The paper is divided into two parts: literature review and experimental research. In the part of literature review, the clinical and experimental research progress of liver stagnation and spleen deficiency syndrome, Chaihu Shugan San, Sijunzi decoction and Chaisuxusijun decoction were reviewed. In the experimental research part, the neuroendocrine immune network, the changes of gastrointestinal function, the shuffling liver powder, the Sijunzi decoction and the Chaisusijun decoction on the neuroendocrine immune network in the model of liver stagnation and spleen deficiency syndrome were studied. The effects of Qishusijun decoction on thyroid axis, energy metabolism and blood circulation were discussed. Study 1: establishment of the model of liver stagnation and spleen deficiency: rats were randomly divided into normal group and liver stagnation and spleen deficiency group with 40 rats in each group. The rats in the model group were induced by chronic restraint stress and excessive fatigue diet. The rats were placed in the restraint box for 3 hours at 8:00 every day and swimming in a plastic bucket containing warm water (22 鹵1 鈩，

本文編號：2326606