發(fā)布時間：2018-11-04 11:00

【摘要】：脂多糖(LPS)是革蘭氏陰性細菌外膜的主要組成部分。它能通過對抗抗菌劑來保護細菌的細胞壁。脂多糖是包含疏水脂質(zhì)和親水多糖兩部分的異聚物。在溶菌后，胞壁的LPS被釋放入血液循環(huán)系統(tǒng)，通過刺激單核細胞、巨噬細胞、中性粒細胞、血小板及內(nèi)皮細胞而激活免疫系統(tǒng)。脂多糖還能綁定到血漿白蛋白形成復(fù)合物，增加LPS的毒性以及LPS對膜受體的親和力。LPS刺激免疫細胞后導(dǎo)致炎癥因子的釋放，如：細胞活素類、化學(xué)增活素、酶類、花生四烯酸類物質(zhì)、粘著劑以及自由基，這些物質(zhì)對炎癥反應(yīng)過程起著重要的作用，而且能夠誘導(dǎo)包括膿毒性休克在內(nèi)的病理生理學(xué)過程。因此，研究脂多糖應(yīng)答分子對解決LPS引起的包括膿毒性休克在內(nèi)的一系列病理生理學(xué)過程具有重要的意義。 為此，，課題組致力于探討LPS刺激后應(yīng)答基因的功能，尤其是一些新發(fā)現(xiàn)基因的功能。lrp是一種LPS應(yīng)答基因。系本課題組柴玉波博士采用基于PCR的改良消減雜交技術(shù)，構(gòu)建LPS刺激后人牙髓細胞差異表達基因文庫，從中篩選出的新基因，GenBank登錄號為AF143740。后來在GenBank又有更為完整的
[Abstract]:Lipopolysaccharide (LPS) is a major component of the outer membrane of Gram-negative bacteria. It protects the cell walls of bacteria by anti-antimicrobial agents. Lipopolysaccharide is an heteropolymer consisting of hydrophobic lipids and hydrophilic polysaccharides. After bacteriolysis, LPS from the cell wall is released into the circulatory system, activating the immune system by stimulating monocytes, macrophages, neutrophils, platelets and endothelial cells. Lipopolysaccharide also binds to plasma albumin to form complexes that increase the toxicity of LPS and the affinity of LPS to membrane receptors. LPS stimulates the release of inflammatory cytokines such as cytokines, chemoactivins and enzymes. Arachidonic acids adhesives and free radicals play an important role in inflammatory processes and induce pathophysiological processes including septic shock. Therefore, the study of lipopolysaccharide responsive molecules plays an important role in solving a series of pathophysiological processes, including septic shock caused by LPS. Therefore, the research group is devoted to study the function of the response gene after LPS stimulation, especially the function of some newly discovered genes. Lrp is a kind of LPS response gene. By using improved subtractive hybridization based on PCR, Dr Chai Yubo, our research group, constructed the differentially expressed gene library of human dental pulp cells stimulated by LPS. A new gene, GenBank accession number, AF143740. was selected from the library. Later, at GenBank, there was a more complete
【學(xué)位授予單位】：第四軍醫(yī)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2006
【分類號】：R346

【參考文獻】

相關(guān)期刊論文 前3條

1 蔣建新 ,朱佩芳 ,王正國;內(nèi)毒素的跨膜信號轉(zhuǎn)導(dǎo)及其在全身炎癥反應(yīng)綜合征中的作用[J];解放軍醫(yī)學(xué)雜志;2002年01期

2 陳潤生;當(dāng)前生物信息學(xué)的重要研究任務(wù)[J];生物工程進展;1999年04期

3 李鳴真;腸道細菌和內(nèi)毒素的易位[J];中國危重病急救醫(yī)學(xué);1998年12期

本文編號：2309617