【摘要】：目的探討CD40信號(hào)激活對(duì)腎小管上皮細(xì)胞(HK2)的作用。方法以不同劑量的CD40激發(fā)型單克隆抗體抗(5c11)刺激HK2細(xì)胞,以四甲基偶氮唑鹽(MTT)法和流式細(xì)胞術(shù)檢測(cè)細(xì)胞增殖和凋亡,以酶聯(lián)免疫吸附法檢測(cè)HK2培養(yǎng)中趨化因子受激活調(diào)節(jié)正常T細(xì)胞表達(dá)和分泌因子(RANTES)水平。結(jié)果 HK2表面CD40表達(dá)高達(dá)78.9%。在不同濃度5c11刺激下,第9天時(shí)隨5c11濃度增加HK2細(xì)胞增殖(OD值)逐步下降,各組間的差異均有統(tǒng)計(jì)學(xué)意義(P0.01);第6、第9天時(shí)HK2凋亡率升高,差異有統(tǒng)計(jì)學(xué)意義(P0.01),RANTES分泌隨5c11濃度增加而升高,差異有統(tǒng)計(jì)學(xué)意義(P均0.05),并隨時(shí)間推移呈增加趨勢(shì),差異有統(tǒng)計(jì)學(xué)意義(P均0.01)。結(jié)論 CD40信號(hào)在HK2細(xì)胞中的表達(dá)在導(dǎo)致腎固有細(xì)胞凋亡以及效應(yīng)性細(xì)胞因子的分泌過(guò)程中起重要調(diào)節(jié)作用。
[Abstract]:Objective to investigate the effect of CD40 signal activation on renal tubular epithelial cells (HK2). Methods HK2 cells were stimulated with different doses of CD40 activated monoclonal antibody (5c11). The proliferation and apoptosis of HK2 cells were detected by (MTT) and flow cytometry. Enzyme linked immunosorbent assay (Elisa) was used to detect the level of normal T cell expression and secretion factor (RANTES) in HK2 culture. Results the expression of CD40 on HK2 was 78.9%. On the 9th day, the proliferation (OD) of HK2 cells decreased gradually with the increase of 5c11 concentration under different concentrations of 5c11, and the differences among each group were statistically significant (P0.01). On the 6th and 9th day, the apoptosis rate of HK2 increased, and the difference was statistically significant (P0.01), RANTES secretion increased with the increase of 5c11 concentration (P0. 05). The difference was statistically significant (P 0.01). Conclusion the expression of CD40 signal in HK2 cells plays an important role in the apoptosis of renal intrinsic cells and the secretion of effector cytokines.
【作者單位】： 上海市同濟(jì)大學(xué)附屬第十人民醫(yī)院兒科;江蘇省蘇州市蘇州大學(xué)附屬兒童醫(yī)院腎內(nèi)科;
【分類(lèi)號(hào)】：R692

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