CD40信號激活對腎小管上皮細胞的影響
發(fā)布時間：2018-11-03 11:49
【摘要】：目的探討CD40信號激活對腎小管上皮細胞(HK2)的作用。方法以不同劑量的CD40激發(fā)型單克隆抗體抗(5c11)刺激HK2細胞,以四甲基偶氮唑鹽(MTT)法和流式細胞術(shù)檢測細胞增殖和凋亡,以酶聯(lián)免疫吸附法檢測HK2培養(yǎng)中趨化因子受激活調(diào)節(jié)正常T細胞表達和分泌因子(RANTES)水平。結(jié)果 HK2表面CD40表達高達78.9%。在不同濃度5c11刺激下,第9天時隨5c11濃度增加HK2細胞增殖(OD值)逐步下降,各組間的差異均有統(tǒng)計學(xué)意義(P0.01);第6、第9天時HK2凋亡率升高,差異有統(tǒng)計學(xué)意義(P0.01),RANTES分泌隨5c11濃度增加而升高,差異有統(tǒng)計學(xué)意義(P均0.05),并隨時間推移呈增加趨勢,差異有統(tǒng)計學(xué)意義(P均0.01)。結(jié)論 CD40信號在HK2細胞中的表達在導(dǎo)致腎固有細胞凋亡以及效應(yīng)性細胞因子的分泌過程中起重要調(diào)節(jié)作用。
[Abstract]:Objective to investigate the effect of CD40 signal activation on renal tubular epithelial cells (HK2). Methods HK2 cells were stimulated with different doses of CD40 activated monoclonal antibody (5c11). The proliferation and apoptosis of HK2 cells were detected by (MTT) and flow cytometry. Enzyme linked immunosorbent assay (Elisa) was used to detect the level of normal T cell expression and secretion factor (RANTES) in HK2 culture. Results the expression of CD40 on HK2 was 78.9%. On the 9th day, the proliferation (OD) of HK2 cells decreased gradually with the increase of 5c11 concentration under different concentrations of 5c11, and the differences among each group were statistically significant (P0.01). On the 6th and 9th day, the apoptosis rate of HK2 increased, and the difference was statistically significant (P0.01), RANTES secretion increased with the increase of 5c11 concentration (P0. 05). The difference was statistically significant (P 0.01). Conclusion the expression of CD40 signal in HK2 cells plays an important role in the apoptosis of renal intrinsic cells and the secretion of effector cytokines.
【作者單位】： 上海市同濟大學(xué)附屬第十人民醫(yī)院兒科;江蘇省蘇州市蘇州大學(xué)附屬兒童醫(yī)院腎內(nèi)科;
【分類號】：R692
[Abstract]:Objective to investigate the effect of CD40 signal activation on renal tubular epithelial cells (HK2). Methods HK2 cells were stimulated with different doses of CD40 activated monoclonal antibody (5c11). The proliferation and apoptosis of HK2 cells were detected by (MTT) and flow cytometry. Enzyme linked immunosorbent assay (Elisa) was used to detect the level of normal T cell expression and secretion factor (RANTES) in HK2 culture. Results the expression of CD40 on HK2 was 78.9%. On the 9th day, the proliferation (OD) of HK2 cells decreased gradually with the increase of 5c11 concentration under different concentrations of 5c11, and the differences among each group were statistically significant (P0.01). On the 6th and 9th day, the apoptosis rate of HK2 increased, and the difference was statistically significant (P0.01), RANTES secretion increased with the increase of 5c11 concentration (P0. 05). The difference was statistically significant (P 0.01). Conclusion the expression of CD40 signal in HK2 cells plays an important role in the apoptosis of renal intrinsic cells and the secretion of effector cytokines.
【作者單位】： 上海市同濟大學(xué)附屬第十人民醫(yī)院兒科;江蘇省蘇州市蘇州大學(xué)附屬兒童醫(yī)院腎內(nèi)科;
【分類號】：R692
【參考文獻】
相關(guān)期刊論文 前1條
1 石向華;劉小友;孫煦勇;趙明;;CD40阻斷劑對大鼠腎移植急性排斥反應(yīng)的影響[J];南方醫(yī)科大學(xué)學(xué)報;2011年12期
【共引文獻】
相關(guān)期刊論文 前10條
1 王芳;張伯科;王顯;;雷洛昔芬對多柔比星誘導(dǎo)大鼠蛋白尿腎損害的作用及部分機制研究[J];安徽醫(yī)藥;2010年11期
2 馮博;劉文軍;徐萬忠;路曉光;劉瑞華;;益氣活血降濁方對TGF-β 1誘導(dǎo)人近端腎小管上皮細胞HGF、BMP-7表達的影響[J];北京中醫(yī)藥;2012年03期
3 李國霞;腎小球疾病中腎小管間質(zhì)損害發(fā)病機制研究進展[J];承德醫(yī)學(xué)院學(xué)報;2005年03期
4 鄧劍波,王代紅,張耀全,袁發(fā)煥;慢性腎臟病患者腎組織熱休克蛋白47的表達及意義[J];重慶醫(yī)學(xué);2005年07期
5 張瑞;張靜波;陳永文;周鏡然;袁發(fā)煥;;體外組裝補體膜攻擊復(fù)合物對腎小管上皮細胞Ⅳ型膠原合成的影響[J];重慶醫(yī)學(xué);2007年05期
6 陳幼發(fā);熊羽;;全反式維甲酸對糖尿病大鼠腎小管-間質(zhì)纖維化的作用及機制[J];重慶醫(yī)學(xué);2009年15期
7 周寶尚;張t，
本文編號：2307702
本文鏈接：http://sikaile.net/yixuelunwen/binglixuelunwen/2307702.html
最近更新
教材專著
