狂犬病毒脂質(zhì)體疫苗的研究
發(fā)布時間：2018-10-26 15:03
【摘要】：目前廣泛應(yīng)用的狂犬病疫苗為VERO細胞純化滅活疫苗,以氫氧化鋁做為佐劑,需免疫五次,且保護效力較低。本文制備了狂犬病毒脂質(zhì)體疫苗,以期減少免疫次數(shù),并且提高疫苗的保護效力。 我們以氫化大豆磷脂、膽固醇、十八胺為原料制備空白脂質(zhì)體,以海藻糖為凍干保護劑。采用凍干重建法(REV-DRV)制備狂犬病毒脂質(zhì)體疫苗。采用凍干重建法制備的狂犬病毒脂質(zhì)體疫苗形態(tài)成圓形或橢圓形,粒徑分布均勻,平均粒徑在12.25μm左右,包封率在65%以上。本品無刺激性,安全試驗、過敏試驗均合格。以脂質(zhì)體為載體的狂犬疫苗同鋁佐劑疫苗相比,可提高效力3倍,中和抗體脂質(zhì)體疫苗組明顯高于對照鋁佐劑組,兩組之間相比有顯著性差異。采用凍干重建法制備的狂犬病毒脂質(zhì)體疫苗可同時增強細胞免疫和體液免疫,提高免疫效力,有望減少免疫針次。
[Abstract]:The widely used rabies vaccine is purified inactivated VERO cell vaccine. Aluminum hydroxide as adjuvant needs to be immunized five times and the protective efficacy is low. The rabies virus liposome vaccine was prepared in order to reduce the immunization times and improve the protective effect of the vaccine. Blank liposomes were prepared from hydrogenated soybean phospholipid, cholesterol and octadecylamine, and trehalose was used as lyophilization protectant. Liposome vaccine against rabies virus was prepared by freeze-dried reconstruction (REV-DRV). The rabies virus liposome vaccine prepared by freeze-drying reconstruction method is round or elliptical in shape. The particle size distribution is uniform, the average particle size is about 12.25 渭 m, and the encapsulation efficiency is over 65%. This product has no irritation, safety test and allergy test. Compared with aluminum adjuvant vaccine, rabies vaccine with liposome as carrier could increase the potency by three times. The neutralizing antibody liposome vaccine group was significantly higher than that of control aluminum adjuvant group, and there was significant difference between the two groups. The liposome vaccine of rabies virus prepared by freeze-dried reconstruction can enhance both cellular and humoral immunity and improve the immune efficacy.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2006
【分類號】：R392
本文編號：2296106
[Abstract]:The widely used rabies vaccine is purified inactivated VERO cell vaccine. Aluminum hydroxide as adjuvant needs to be immunized five times and the protective efficacy is low. The rabies virus liposome vaccine was prepared in order to reduce the immunization times and improve the protective effect of the vaccine. Blank liposomes were prepared from hydrogenated soybean phospholipid, cholesterol and octadecylamine, and trehalose was used as lyophilization protectant. Liposome vaccine against rabies virus was prepared by freeze-dried reconstruction (REV-DRV). The rabies virus liposome vaccine prepared by freeze-drying reconstruction method is round or elliptical in shape. The particle size distribution is uniform, the average particle size is about 12.25 渭 m, and the encapsulation efficiency is over 65%. This product has no irritation, safety test and allergy test. Compared with aluminum adjuvant vaccine, rabies vaccine with liposome as carrier could increase the potency by three times. The neutralizing antibody liposome vaccine group was significantly higher than that of control aluminum adjuvant group, and there was significant difference between the two groups. The liposome vaccine of rabies virus prepared by freeze-dried reconstruction can enhance both cellular and humoral immunity and improve the immune efficacy.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2006
【分類號】：R392
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相關(guān)期刊論文 前4條
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，本文編號：2296106
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