【摘要】： 阻塞性黃疸(簡稱阻黃)是由于肝內(nèi)、外膽管的機械性因素造成的膽汁瘀積、繼而導致全身多系統(tǒng)病變的臨床病癥。阻黃及其引起的并發(fā)癥與腸道菌群密切相關。本文采用常規(guī)的膽管結扎方法對SD大鼠進行阻黃造模,主要對手術前后的腸道菌群結構的變化進行了分子生態(tài)學研究,并與假手術組大鼠進行比較。采集膽管結扎組(n=5)及假手術組(n=5)大鼠手術前3天,手術后3天、7天、兩周共四個時間點的糞便樣品,提取糞便樣品的總DNA,采用ERIC-PCR指紋圖譜、16S rRNA基因V3區(qū)PCR-DGGE和針對4類腸道主要菌群(乳酸菌、梭菌、雙歧桿菌、擬桿菌)的類群特異性PCR- DGGE并結合主成分分析方法對樣品微生物結構組成進行深入分析。 ERIC-PCR分析結果表明,大鼠腸道菌群的組成具有宿主專一性,模型組大鼠腸道微生物群落多樣性于膽管結扎后7天明顯下降,原來的優(yōu)勢條帶更加明顯,而假手術組未出現(xiàn)此類情況。 對膽管結扎組與假手術組大鼠腸道菌群的16S rRNA基因V3區(qū)DGGE及其主成分分析(PCA)表明:(1)手術前大鼠腸道主要菌群的組成雖然具有個體差異性,但基本上聚在一起,并明顯區(qū)分于手術后的大鼠。(2)手術后兩周,膽管結扎組大鼠腸道主要菌群發(fā)生了不同于假手術組的變
[Abstract]:Obstructive jaundice is a clinical disease caused by mechanical factors of intrahepatic and extrahepatic bile duct, which leads to systemic multisystem lesions. The obstruction of yellow and its complications are closely related to the intestinal flora. In this paper, a routine bile duct ligation method was used to model SD rats. The changes of intestinal flora structure before and after operation were studied in molecular ecology, and compared with those in sham operation group. The fecal samples were collected from rats in bile duct ligation group (nong5) and sham operation group (nong5) 3 days before operation, 3 days after operation, 7 days after operation and 2 weeks. Total DNA, of fecal samples was extracted by ERIC-PCR fingerprinting, 16s rRNA V3 PCR-DGGE and four main intestinal flora (lactic acid bacteria, Clostridium spp., Bifidobacterium). Group specific PCR- DGGE and principal component analysis (PCA) were used to analyze the microbial structure of the samples. The results of ERIC-PCR analysis showed that, The composition of intestinal flora of rats was host specific. The diversity of intestinal microbial communities in the model group decreased significantly 7 days after bile duct ligation, and the original dominant bands were more obvious than those in the sham operation group. The DGGE of the V3 region of 16s rRNA gene in the intestinal flora of rats in bile duct ligation group and sham operation group and its principal component analysis (PCA) showed that: (1) although the composition of main intestinal flora of rats before operation had individual differences, it was basically clustered together. (2) two weeks after operation, the main intestinal flora in bile duct ligation group was different from that in sham operation group. (2) two weeks after operation, the main intestinal flora in bile duct ligation group was different from that in sham operation group.
【學位授予單位】：上海交通大學
【學位級別】：碩士
【學位授予年份】：2007
【分類號】：R378;R575

【引證文獻】

相關期刊論文 前1條

1 李文賓;吳敏麗;廉振民;;分子生態(tài)學應用研究現(xiàn)狀[J];中國微生態(tài)學雜志;2009年01期

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本文編號：2276022