【摘要】：骨髓是一個(gè)具有多種細(xì)胞成分的復(fù)雜器官,有造血及成骨能力。骨髓細(xì)胞的成骨能力來源于骨髓基質(zhì)系統(tǒng)的基質(zhì)干細(xì)胞(Stromalstem cell),在體外,骨髓基質(zhì)細(xì)胞的成骨作用已得到肯定。由于骨髓具有取材方便、對(duì)機(jī)體損傷小的特點(diǎn),成為骨組織工程最有應(yīng)用前景的種子細(xì)胞。但骨髓基質(zhì)細(xì)胞具有多分化潛能,且有多種成分,因此,骨髓基質(zhì)細(xì)胞的純化和對(duì)其定向誘導(dǎo)分化為成骨細(xì)胞,是其應(yīng)用于骨組織工程的前提。本實(shí)驗(yàn)是在動(dòng)物實(shí)驗(yàn)的基礎(chǔ)上,探討一種通過換液純化骨髓基質(zhì)細(xì)胞,使用不同濃度地塞米松以尋找一對(duì)骨髓基質(zhì)細(xì)胞的增殖和分化均較適宜的濃度,并在適宜濃度的條件培養(yǎng)基條件下使其定向分化,旨在建立一種簡便易行的成人骨髓基質(zhì)細(xì)胞體外培養(yǎng)方法,為進(jìn)一步研究成骨細(xì)胞及骨組織工程提供技術(shù)平臺(tái)。 目的:建立一種成人骨髓基質(zhì)細(xì)胞在體外培養(yǎng)條件向成骨細(xì)胞轉(zhuǎn)化的方法,探討簡易的成人成骨細(xì)胞體外培養(yǎng)方法,為進(jìn)一步研究成骨細(xì)胞及骨組織工程提供技術(shù)平臺(tái)。 方法:手術(shù)中抽取健康成人骨髓組織,采用全骨髓培養(yǎng)法在體外進(jìn)行培養(yǎng),細(xì)胞匯合后改用含不同濃度地塞米松的培養(yǎng)基培養(yǎng)后觀察細(xì)胞的增殖和分化情況。再采用適宜濃度地塞米松、β-甘油磷酸鈉和維生素C的條件培養(yǎng)基進(jìn)行傳代培養(yǎng),觀察細(xì)胞增殖和分化情況,并對(duì)細(xì)胞進(jìn)行鑒定。 結(jié)果:通過對(duì)不同濃度地塞米松對(duì)骨髓基質(zhì)細(xì)胞增殖與分化的影響觀察,認(rèn)為10~(-8)mol/L的濃度為比較合適的濃度。并采用全骨髓法在體外培養(yǎng)的成人成骨細(xì)胞表現(xiàn)成骨細(xì)胞的形態(tài)特征,細(xì)胞ALP陽性
[Abstract]:Bone marrow is a complex organ with a variety of cellular components and has the ability of hematopoiesis and osteogenesis. The osteogenic ability of bone marrow cells comes from the stromal stem cells of bone marrow stromal system. In vitro, the osteogenic effect of bone marrow stromal cells has been confirmed. Bone marrow stromal cells (BMSCs) have multiple differentiation potentials and various components. Therefore, the purification and directional differentiation of BMSCs into osteoblasts are the prerequisites for bone tissue engineering. The aim of this study is to establish a simple and convenient method for in vitro culture of adult bone marrow stromal cells (BMSCs) by using dexamethasone at different concentrations in order to find a suitable concentration for proliferation and differentiation of a pair of BMSCs and to differentiate them into osteoblasts and bone groups. Weaving technology provides technical platform.
AIM: To establish a method for the transformation of adult bone marrow stromal cells (BMSCs) into osteoblasts in vitro, and to explore a simple method for the culture of adult osteoblasts in vitro, so as to provide a technical platform for the further study of osteoblasts and bone tissue engineering.
Methods: Bone marrow tissues of healthy adults were extracted during operation and cultured in vitro by whole bone marrow culture method. Cells were cultured in dexamethasone medium containing different concentrations of dexamethasone and then subcultured in dexamethasone, sodium beta glycerophosphate and vitamin C medium. The cell proliferation and differentiation were observed, and the cells were identified.
RESULTS: By observing the effect of dexamethasone on the proliferation and differentiation of bone marrow stromal cells, the concentration of 10~(-8) mol/L was considered to be the most suitable concentration.
【學(xué)位授予單位】：新疆醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2005
【分類號(hào)】：R329.2

【引證文獻(xiàn)】

相關(guān)期刊論文 前1條

1 李溪;劉勁松;吳仕峰;趙峻;;探索大鼠骨髓基質(zhì)干細(xì)胞體外培養(yǎng)的方法[J];昆明醫(yī)學(xué)院學(xué)報(bào);2007年05期

相關(guān)碩士學(xué)位論文 前1條

1 魏彩霞;雞精原干細(xì)胞體外培養(yǎng)及其誘導(dǎo)分化的研究[D];揚(yáng)州大學(xué);2007年

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本文編號(hào)：2245069