【摘要】：骨髓是一個具有多種細胞成分的復(fù)雜器官,有造血及成骨能力。骨髓細胞的成骨能力來源于骨髓基質(zhì)系統(tǒng)的基質(zhì)干細胞(Stromalstem cell),在體外,骨髓基質(zhì)細胞的成骨作用已得到肯定。由于骨髓具有取材方便、對機體損傷小的特點,成為骨組織工程最有應(yīng)用前景的種子細胞。但骨髓基質(zhì)細胞具有多分化潛能,且有多種成分,因此,骨髓基質(zhì)細胞的純化和對其定向誘導(dǎo)分化為成骨細胞,是其應(yīng)用于骨組織工程的前提。本實驗是在動物實驗的基礎(chǔ)上,探討一種通過換液純化骨髓基質(zhì)細胞,使用不同濃度地塞米松以尋找一對骨髓基質(zhì)細胞的增殖和分化均較適宜的濃度,并在適宜濃度的條件培養(yǎng)基條件下使其定向分化,旨在建立一種簡便易行的成人骨髓基質(zhì)細胞體外培養(yǎng)方法,為進一步研究成骨細胞及骨組織工程提供技術(shù)平臺。 目的:建立一種成人骨髓基質(zhì)細胞在體外培養(yǎng)條件向成骨細胞轉(zhuǎn)化的方法,探討簡易的成人成骨細胞體外培養(yǎng)方法,為進一步研究成骨細胞及骨組織工程提供技術(shù)平臺。 方法:手術(shù)中抽取健康成人骨髓組織,采用全骨髓培養(yǎng)法在體外進行培養(yǎng),細胞匯合后改用含不同濃度地塞米松的培養(yǎng)基培養(yǎng)后觀察細胞的增殖和分化情況。再采用適宜濃度地塞米松、β-甘油磷酸鈉和維生素C的條件培養(yǎng)基進行傳代培養(yǎng),觀察細胞增殖和分化情況,并對細胞進行鑒定。 結(jié)果:通過對不同濃度地塞米松對骨髓基質(zhì)細胞增殖與分化的影響觀察,認為10~(-8)mol/L的濃度為比較合適的濃度。并采用全骨髓法在體外培養(yǎng)的成人成骨細胞表現(xiàn)成骨細胞的形態(tài)特征,細胞ALP陽性
[Abstract]:Bone marrow is a complex organ with a variety of cellular components and has the ability of hematopoiesis and osteogenesis. The osteogenic ability of bone marrow cells comes from the stromal stem cells of bone marrow stromal system. In vitro, the osteogenic effect of bone marrow stromal cells has been confirmed. Bone marrow stromal cells (BMSCs) have multiple differentiation potentials and various components. Therefore, the purification and directional differentiation of BMSCs into osteoblasts are the prerequisites for bone tissue engineering. The aim of this study is to establish a simple and convenient method for in vitro culture of adult bone marrow stromal cells (BMSCs) by using dexamethasone at different concentrations in order to find a suitable concentration for proliferation and differentiation of a pair of BMSCs and to differentiate them into osteoblasts and bone groups. Weaving technology provides technical platform.
AIM: To establish a method for the transformation of adult bone marrow stromal cells (BMSCs) into osteoblasts in vitro, and to explore a simple method for the culture of adult osteoblasts in vitro, so as to provide a technical platform for the further study of osteoblasts and bone tissue engineering.
Methods: Bone marrow tissues of healthy adults were extracted during operation and cultured in vitro by whole bone marrow culture method. Cells were cultured in dexamethasone medium containing different concentrations of dexamethasone and then subcultured in dexamethasone, sodium beta glycerophosphate and vitamin C medium. The cell proliferation and differentiation were observed, and the cells were identified.
RESULTS: By observing the effect of dexamethasone on the proliferation and differentiation of bone marrow stromal cells, the concentration of 10~(-8) mol/L was considered to be the most suitable concentration.
【學位授予單位】：新疆醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2005
【分類號】：R329.2

【引證文獻】

相關(guān)期刊論文 前1條

1 李溪;劉勁松;吳仕峰;趙峻;;探索大鼠骨髓基質(zhì)干細胞體外培養(yǎng)的方法[J];昆明醫(yī)學院學報;2007年05期

相關(guān)碩士學位論文 前1條

1 魏彩霞;雞精原干細胞體外培養(yǎng)及其誘導(dǎo)分化的研究[D];揚州大學;2007年

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本文編號：2245069