【摘要】：骨髓是一個(gè)具有多種細(xì)胞成分的復(fù)雜器官,有造血及成骨能力。骨髓細(xì)胞的成骨能力來源于骨髓基質(zhì)系統(tǒng)的基質(zhì)干細(xì)胞(Stromalstem cell),在體外,骨髓基質(zhì)細(xì)胞的成骨作用已得到肯定。由于骨髓具有取材方便、對機(jī)體損傷小的特點(diǎn),成為骨組織工程最有應(yīng)用前景的種子細(xì)胞。但骨髓基質(zhì)細(xì)胞具有多分化潛能,且有多種成分,因此,骨髓基質(zhì)細(xì)胞的純化和對其定向誘導(dǎo)分化為成骨細(xì)胞,是其應(yīng)用于骨組織工程的前提。本實(shí)驗(yàn)是在動(dòng)物實(shí)驗(yàn)的基礎(chǔ)上,探討一種通過換液純化骨髓基質(zhì)細(xì)胞,使用不同濃度地塞米松以尋找一對骨髓基質(zhì)細(xì)胞的增殖和分化均較適宜的濃度,并在適宜濃度的條件培養(yǎng)基條件下使其定向分化,旨在建立一種簡便易行的成人骨髓基質(zhì)細(xì)胞體外培養(yǎng)方法,為進(jìn)一步研究成骨細(xì)胞及骨組織工程提供技術(shù)平臺。 目的:建立一種成人骨髓基質(zhì)細(xì)胞在體外培養(yǎng)條件向成骨細(xì)胞轉(zhuǎn)化的方法,探討簡易的成人成骨細(xì)胞體外培養(yǎng)方法,為進(jìn)一步研究成骨細(xì)胞及骨組織工程提供技術(shù)平臺。 方法:手術(shù)中抽取健康成人骨髓組織,采用全骨髓培養(yǎng)法在體外進(jìn)行培養(yǎng),細(xì)胞匯合后改用含不同濃度地塞米松的培養(yǎng)基培養(yǎng)后觀察細(xì)胞的增殖和分化情況。再采用適宜濃度地塞米松、β-甘油磷酸鈉和維生素C的條件培養(yǎng)基進(jìn)行傳代培養(yǎng),觀察細(xì)胞增殖和分化情況,并對細(xì)胞進(jìn)行鑒定。 結(jié)果:通過對不同濃度地塞米松對骨髓基質(zhì)細(xì)胞增殖與分化的影響觀察,認(rèn)為10~(-8)mol/L的濃度為比較合適的濃度。并采用全骨髓法在體外培養(yǎng)的成人成骨細(xì)胞表現(xiàn)成骨細(xì)胞的形態(tài)特征,細(xì)胞ALP陽性
[Abstract]:Bone marrow is a complex organ with multiple cellular components, with hematopoietic and osteogenic capacity. The osteogenic ability of bone marrow cells is derived from stromal stem cell (Stromalstem cell), of bone marrow stromal system in vitro, and the osteogenic effect of bone marrow stromal cells has been confirmed. Bone marrow is the most promising seed cell for bone tissue engineering because of its advantages of easy extraction and less damage to body. However, bone marrow stromal cells have multiple differentiation potential and many components. Therefore, the purification and directional induction of bone marrow stromal cells into osteoblasts is the premise of its application in bone tissue engineering. On the basis of animal experiments, we studied a suitable concentration of dexamethasone for the proliferation and differentiation of bone marrow stromal cells (BMSCs), which were purified by fluid exchange, and used dexamethasone in different concentrations to find out the suitable concentration for the proliferation and differentiation of bone marrow stromal cells. In order to establish a simple culture method of adult bone marrow stromal cells in vitro and provide a technical platform for further study of osteoblasts and bone tissue engineering. Objective: to establish a method of transforming adult bone marrow stromal cells into osteoblasts in vitro, and to explore a simple method of adult osteoblasts culture in vitro, and to provide a technical platform for further research on osteoblasts and bone tissue engineering. Methods: the healthy adult bone marrow tissue was extracted during operation and cultured in vitro by whole bone marrow culture method. The proliferation and differentiation of the cells were observed after the cells were confluent and cultured in different concentrations of dexamethasone medium. Then the cells were subcultured in the suitable concentration of dexamethasone, 尾 -glycerophosphate and vitamin C, the proliferation and differentiation of the cells were observed, and the cells were identified. Results: the effects of different concentrations of dexamethasone on proliferation and differentiation of bone marrow stromal cells were observed. It was concluded that the concentration of 10 ~ (-8) mol/L was the appropriate concentration. The adult osteoblasts cultured in vitro showed the morphological characteristics of osteoblasts and ALP positive cells by whole bone marrow method.
【學(xué)位授予單位】：新疆醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2005
【分類號】：R329.2

【引證文獻(xiàn)】

相關(guān)期刊論文 前1條

1 李溪;劉勁松;吳仕峰;趙峻;;探索大鼠骨髓基質(zhì)干細(xì)胞體外培養(yǎng)的方法[J];昆明醫(yī)學(xué)院學(xué)報(bào);2007年05期

相關(guān)碩士學(xué)位論文 前1條

1 魏彩霞;雞精原干細(xì)胞體外培養(yǎng)及其誘導(dǎo)分化的研究[D];揚(yáng)州大學(xué);2007年

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本文編號：2245068