【摘要】：神經(jīng)生長因子(nerve growth factor,NGF)不僅對正在發(fā)育的神經(jīng)系統(tǒng)中神經(jīng)元的存活、分化和成熟等方面產(chǎn)生重要影響，而且在動物的整個生命過程中起著廣泛的作用。近年來，有許多研究證明，，NGF對受刺激或損傷的背根神經(jīng)節(jié)(dorsal root ganglion，DRG)神經(jīng)元、脊髓神經(jīng)元結(jié)構(gòu)及功能的可塑性具有影響，NGF能夠介導DRG神經(jīng)元和脊髓神經(jīng)元的細胞內(nèi)事件，如神經(jīng)元突起內(nèi)線粒體的運輸或聚集以及細胞內(nèi)鈣離子濃度的變化等。神經(jīng)系統(tǒng)受損傷以后NGF的表達增加，這對于神經(jīng)系統(tǒng)正常結(jié)構(gòu)和功能的維持是必須的�？傊�，NGF對受損傷的神經(jīng)元具有保護作用。單涎性神經(jīng)節(jié)苷脂(monosiaganglioside，GM1)在神經(jīng)系統(tǒng)發(fā)育過程中，在含量上具有顯著的變化。體內(nèi)與體外實驗皆表明，神經(jīng)細胞分化過程中伴隨著GM1生物合成的改變。神經(jīng)細胞誘導分化過程中伴有核膜GM1含量的增高。GM1通過影響由多肽生長因子調(diào)控的細胞增殖和促成熟過程而調(diào)節(jié)細胞生長。在DRG神經(jīng)元突起的發(fā)芽和生長過程中，NGF和GM1共同作用介導了這一過程。聯(lián)合應用NGF和GM1對神經(jīng)元的保護作用機制目前尚不清楚。因此，本研究將原代分散培養(yǎng)的胎鼠DRG神經(jīng)元和脊髓神經(jīng)元，用谷氨酸(2mmol／L)造成神經(jīng)元損傷，觀察聯(lián)合應用NGF和GM1對體外培養(yǎng)神經(jīng)元損傷的保護作用，并探討其作用機制；將成年大鼠坐骨神經(jīng)造成缺損，使其相應節(jié)段的DRG神經(jīng)元和脊髓前角神經(jīng)元產(chǎn)生損傷，探討聯(lián)合應用NGF和GM1對在體神經(jīng)元損傷的保護作用。結(jié)果表明聯(lián)合應用NGF和GM1對DRG神經(jīng)元和脊髓神經(jīng)元的保護作用效果明顯優(yōu)于單純應用
[Abstract]:Nerve growth factor (nerve growth factor,NGF) not only plays an important role in the survival, differentiation and maturation of neurons in the developing nervous system, but also plays an important role in the whole life process of animals. In recent years, many studies have shown that NGF has an effect on the plasticity of the structure and function of the stimulated or injured dorsal root ganglion (dorsal root ganglion,DRG) neurons and spinal cord neurons. NGF can mediate intracellular events in DRG neurons and spinal cord neurons. Such as the transport or aggregation of mitochondria in neurite and the change of intracellular calcium concentration. The expression of NGF is increased after nervous system injury, which is necessary for the maintenance of normal structure and function of nervous system. NGF can protect injured neurons. Monolabar ganglioside (monosiaganglioside,GM1) has a significant change in the content of the nervous system during the development of the nervous system. In vivo and in vitro experiments showed that GM1 biosynthesis was associated with neuronal differentiation. During the induction of neuronal differentiation, the nuclear membrane GM1 content is increased. GM1 regulates cell growth by affecting the proliferation and maturation process regulated by polypeptide growth factor. This process is mediated by the interaction of DRG and GM1 during the germination and growth of neuronal processes. The protective mechanism of combined use of NGF and GM1 on neurons is unclear. Therefore, the primary cultured fetal DRG neurons and spinal cord neurons were treated with glutamic acid (2mmol/L) to induce neuronal injury. The protective effects of NGF and GM1 on cultured neurons were observed and its mechanism was discussed. The sciatic nerve in adult rats was damaged by DRG neurons and spinal cord anterior horn neurons. The protective effects of NGF and GM1 on in vivo neuronal injury were investigated. The results showed that the protective effect of combined application of NGF and GM1 on DRG neurons and spinal cord neurons was better than that of single application.
【學位授予單位】：山東大學
【學位級別】：博士
【學位授予年份】：2006
【分類號】：R363

【參考文獻】

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1 張引成,王貴和,張政華;神經(jīng)節(jié)苷脂M_1介導神經(jīng)生長因子對運動神經(jīng)元再生的影響[J];西安醫(yī)科大學學報;2001年05期

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