【摘要】：目的：觀察大鼠肝缺血再灌注(I／R)損傷過程中卵巢組織iNOS、MPO、MDA的變化及卵巢組織形態(tài)學(xué)改變，探討卵巢組織能量代謝的變化，內(nèi)分泌變化及其可能的機(jī)制。方法：建立肝缺血再灌注損傷動(dòng)物模型，選健康Wistar大鼠48只，，均為雌鼠，隨機(jī)分為對(duì)照組、缺血30min組(I組)、缺血30min即刻再灌注組(I／R組)、缺血30min再灌注后2h組(I／R2h組)、缺血30min再灌注后4h組(I／R4h組)、缺血30min再灌注后6h組(I／R6h組)，每組8只。應(yīng)用免疫組化法分別檢測(cè)誘導(dǎo)型一氧化氮合酶(iNOS)的表達(dá)情況，同時(shí)行光鏡檢查，檢測(cè)卵巢組織勻漿中髓過氧化物酶(MPO)、丙二醛(MDA)含量的變化，測(cè)定卵巢組織勻漿中ATP酶、乳酸脫氫酶(LDH)的活性及乳酸(LD)的含量，放免法檢測(cè)血清雌二醇(E2)、孕酮(p)含量。結(jié)果：在肝缺血再灌注損傷過程中，iNOS在卵巢組織中的表達(dá)在I／R、I／R2h組與對(duì)照組、I組、I／R4h、I／R6h組比較差異有統(tǒng)計(jì)學(xué)意義(P＜0.01)。光鏡下I／R、IR2h組間質(zhì)輕度水腫，少許炎細(xì)胞浸潤(rùn)，血管擴(kuò)張充血，卵泡內(nèi)卵丘顆粒細(xì)胞輕度變性。I／R4h、I／R6h組間質(zhì)重度水腫，炎細(xì)胞浸潤(rùn)明顯，大量血管擴(kuò)張充血，卵泡內(nèi)卵丘顆粒細(xì)胞明顯變性。MPO在卵巢組織中的含量I、I／R6h組與對(duì)照組、I／R、I／R2h組比較差異有統(tǒng)計(jì)學(xué)意義(P＜0.01，P＜0.05)，MDA在卵巢組織中的含量I、I／R2h、I／R4h、I／R6h組與對(duì)照組比較差異有統(tǒng)計(jì)學(xué)意義(P＜0.01，P＜0.05)，I／R、I／R4h、I／R6h組與I組比較差異有統(tǒng)計(jì)學(xué)意義(P＜0.01，P＜0.05)，I／R4h、I／R6h組與I／R組、I／R2h組比較差異
[Abstract]:Aim: to observe the changes of iNOS,MPO,MDA and morphology of ovarian tissue during hepatic ischemia reperfusion (I / R) injury in rats, and to investigate the changes of energy metabolism, endocrine and its possible mechanism. Methods: an animal model of hepatic ischemia-reperfusion injury was established. 48 healthy Wistar rats, all female rats, were randomly divided into control group. There were 8 rats in each group: 30min group (group I), immediate reperfusion group (group I / R), I/R2h group (group 2), I/R4h group (group I/R4h) and I/R6h group (group I/R6h). The expression of inducible nitric oxide synthase (iNOS) was detected by immunohistochemistry, and the changes of myeloperoxidase (MPO),) malondialdehyde (MDA) (MDA) in ovarian homogenate were detected by light microscopy, and ATP enzyme in ovarian homogenate was measured. The activity of lactate dehydrogenase (LDH) and the content of lactate (LD) were determined by radioimmunoassay. Serum estradiol (E2) and progesterone (p) were detected by radioimmunoassay. Results: there was significant difference in the expression of iNOS in ovarian tissues between the I / R / R 2 h group and the control group (P < 0.01) during hepatic ischemia-reperfusion injury (P < 0.01). The expression of iNOS in the ovarian tissue was significantly higher than that in the control group (P < 0.01). The expression of iNOS in the ovarian tissue was significantly higher than that in the control group (P < 0.01). Under light microscope, I / R IR2h group had mild edema, a little inflammatory cell infiltration, vasodilation, hyperemia, mild degeneration of cumulus granulosa cells in follicles, severe interstitial edema, obvious inflammatory cell infiltration, and massive vascular dilatation and hyperemia. Significant degeneration of cumulus granulosa cells in follicles. MPO content in ovarian tissue. There was significant difference in the content of MDA in ovarian tissues between the I / R / R 6h group and the control group (P < 0.01 P < 0.05). The content of MDA in the ovarian tissue was significantly different between the I / R _ 2 / R _ (6h) group and the control group (P < 0.01 / P < 0.05), and the content of MDA in the ovarian tissue was higher than that in the control group (P < 0.05). There was significant difference between the control group (P < 0.01) and I / R _ (4) H / R _ (6 h) group (P < 0.01 P < 0.05). There was a significant difference between the I / R _ 4 / R _ 4 / R _ (6 h) group and the I _ (R) / R _ (2) h group (P < 0.01 P < 0.05).
【學(xué)位授予單位】：新疆醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2006
【分類號(hào)】：R363

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