發(fā)布時間：2018-08-24 15:01

【摘要】： 漿細(xì)胞樣樹突狀細(xì)胞(pDC)是一類在免疫應(yīng)答與免疫耐受中發(fā)揮重要作用的免疫細(xì)胞,在接受病原刺激后迅速活化,分泌大量的Ⅰ型干擾素,增強(qiáng)或調(diào)控其他免疫細(xì)胞的功能,有效地連接了固有免疫和適應(yīng)性免疫。本課題利用小鼠內(nèi)皮型脾臟基質(zhì)細(xì)胞系模擬體內(nèi)外周淋巴器官內(nèi)免疫應(yīng)答的微環(huán)境,研究了基質(zhì)微環(huán)境對pDC的功能調(diào)節(jié)及其相關(guān)機(jī)制。結(jié)果提示:1.脾臟基質(zhì)微環(huán)境抑制病毒活化的pDC繼續(xù)分泌Ⅰ型干擾素、IL-12、TNF-α和pDC抗原提呈功能,同時抑制了活化pDC對NK細(xì)胞的激活效應(yīng),使NK細(xì)胞分泌IFN-γ下調(diào)。對其機(jī)制的研究表明基質(zhì)細(xì)胞分泌的TGF-β參與了基質(zhì)微環(huán)境對活化pDC的功能調(diào)控;2.脾臟基質(zhì)微環(huán)境中的靜息pDC在接受病毒刺激時仍然迅速活化,分泌高水平Ⅰ型干擾素、IL-12和TNF-α,發(fā)揮獨(dú)特的抗病毒作用。因此,基質(zhì)微環(huán)境對靜息和活化pDC的調(diào)控作用使機(jī)體在免疫防御的同時,維持了免疫自穩(wěn),為臨床上自身免疫疾病、過敏、以及感染等治療方面提供了新的思路。
[Abstract]:Plasma cell like dendritic cell (pDC) is a kind of immune cells that play an important role in immune response and tolerance. After being stimulated by pathogen, plasmacyte-like dendritic cells (pDC) are activated rapidly, secreting a large amount of interferon type I, and enhancing or regulating the function of other immune cells. It effectively connects innate immunity with adaptive immunity. In this study, the mouse endothelial splenic stromal cell line was used to simulate the immune response of peripheral lymphoid organs in vivo and in vivo, and the functional regulation of matrix microenvironment to pDC and its related mechanism were studied. The result suggested that 1: 1. The splenic matrix microenvironment inhibited the viral activation of pDC and continued to secrete IL-12TNF- 偽 and pDC antigen presenting function. It also inhibited the activation effect of activated pDC on NK cells and down-regulated the secretion of IFN- 緯 by NK cells. The study of its mechanism indicated that TGF- 尾 secreted by stromal cells was involved in the regulation of pDC activation by matrix microenvironment. Resting pDC in splenic matrix microenvironment was still activated rapidly and secreted high levels of interferon type I IL-12 and TNF- 偽, which played a unique antiviral role. Therefore, the effect of matrix microenvironment on resting and activating pDC makes the body maintain immune self-stabilization while immune defense, which provides a new idea for the treatment of autoimmune diseases, allergies and infections.
【學(xué)位授予單位】：第二軍醫(yī)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2007
【分類號】：R392

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1 李麗;小鼠脾臟基質(zhì)微環(huán)境對漿細(xì)胞樣樹突狀細(xì)胞的功能調(diào)控作用[D];第二軍醫(yī)大學(xué);2007年

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本文編號：2201191