【摘要】：表皮葡萄球菌(以下簡(jiǎn)稱表葡菌)是引發(fā)醫(yī)院感染的重要致病菌，已得到人們的廣泛關(guān)注。為進(jìn)一步深入了解引發(fā)醫(yī)院感染的表葡菌對(duì)大環(huán)內(nèi)酯-林可酰胺-鏈陽菌素B(MLS_B)的耐藥機(jī)制，本研究收集了2003～2004年北京3家綜合性醫(yī)院126株引發(fā)醫(yī)院感染的表葡菌，檢測(cè)了紅霉素(ERY)、克林霉素(CLI)、克拉霉素(CLR)、頭孢西丁(FOX)和喹奴普丁／達(dá)福普丁對(duì)這些菌株的最小抑菌濃度(MIC)；用D試驗(yàn)區(qū)分誘導(dǎo)型(iMLS_B)和泵出型(MS)耐藥菌株：采用PCR法對(duì)耐藥基因ermA、ermB、ermC、msrA和mecA進(jìn)行了檢測(cè)，，并用脈沖場(chǎng)凝膠電泳(PFGE)進(jìn)行同源性分析。結(jié)果發(fā)現(xiàn)絕大多數(shù)的菌株對(duì)大環(huán)內(nèi)酯類和林可酰胺類抗生素耐藥，但對(duì)喹奴普丁／達(dá)福普丁敏感：結(jié)構(gòu)型(cMLS_B)、誘導(dǎo)型(iMLS_B)和泵出型(MS)MLS_B耐藥菌株分別占73.8％、10.3％和8.7％：在cMLS_B中，耐甲氧西林表葡菌(MRSE)占的比例較高，而在iMLS_B中甲氧西林敏感株(MSSE)較多；攜帶ermA、ermB和ermC的菌株分別占4.27％、2.6％和80.3％，ermC不僅在MRSE和MSSE中是最常見的耐藥基因，而
[Abstract]:Staphylococcus epidermidis (Staphylococcus epidermidis) is an important pathogen causing nosocomial infection. In order to further understand the drug resistance mechanism of nosocomial infection induced by Staphylococcus epibacteria to macrolide-linamide-streptavidin B (MLS_B), 126 strains of S. epidermidis causing nosocomial infection were collected from 2003 to 2004 in 3 general hospitals in Beijing. The minimal inhibitory concentrations (MIC);) of erythromycin (ERY), clindamycin, (CLI), clarithromycin, (CLR), cefoxitin (FOX) and quinuptin / dafopudine against these strains were determined. IMLS_B and (MS) resistant strains were detected by PCR method, and the homology analysis was carried out by pulsed field gel electrophoresis (PFGE). The results showed that most of the strains were resistant to macrolides and lincomas, but sensitive to quinopudine / dafopudine: structural (cMLS_B), inducible (iMLS_B) and pump type (MS) MLS_B) resistant strains accounted for 73. 8% and 8. 7%, respectively. The proportion of methicillin-resistant Staphylococcus epidermidis (MRSE) was higher, but the number of methicillin-sensitive strains (MSSE) was higher in iMLS_B, and that of ermAmin-ermB and ermC were 4.27.6% and 80.3%, respectively, which were not only the most common resistant genes in MRSE and MSSE, but also in MRSE and MSSE.
【學(xué)位授予單位】：第四軍醫(yī)大學(xué)
【學(xué)位級(jí)別】：博士
【學(xué)位授予年份】：2006
【分類號(hào)】：R378

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 熊自忠,李濤,徐元宏;臨床分離耐甲氧西林表皮葡萄球菌的耐藥性監(jiān)測(cè)[J];中國醫(yī)師雜志;2005年02期

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