【摘要】：目的 測(cè)定北京地區(qū)健康人腸道大腸埃希菌耐藥水平和特征；研究整合子和耐藥基因盒在健康人群腸道大腸埃希菌中攜帶情況及分布特點(diǎn)，探討耐藥基因盒與細(xì)菌耐藥表型的關(guān)系；了解整合子在不同基因型菌株中的分布，以及整合子在耐藥性水平傳遞中的作用和分子機(jī)制。 方法 利用全自動(dòng)細(xì)菌鑒定／藥敏分析儀，對(duì)從健康人腸道分離的150株大腸埃希菌進(jìn)行鑒定，14種抗生素敏感性測(cè)定和產(chǎn)ESBLs判讀，另外7種抗生素用紙片擴(kuò)散法進(jìn)行敏感性測(cè)定；對(duì)兩種藥物敏感性方法進(jìn)行平行實(shí)驗(yàn)比較結(jié)果符合率；采用PCR方法檢測(cè)細(xì)菌攜帶Ⅰ、Ⅱ類整合子的情況，，通過限制性內(nèi)切酶分析，純化測(cè)序，將不同大�、耦愓献拥腄NA序列在GeneBank中搜索，確定其可變區(qū)基因盒的種類；利用脈沖場(chǎng)凝膠電泳(PFGE)對(duì)大腸埃希菌進(jìn)行分子分型；通過質(zhì)粒接合試驗(yàn)研究Ⅰ類整合子
[Abstract]:Objective to determine the level and characteristics of drug resistance of Escherichia coli in healthy people in Beijing area. To study the distribution of integron and drug resistance gene box in Escherichia coli in healthy population, to explore the relationship between drug resistance gene box and bacterial phenotype, and to understand the distribution of integron in different genotypes of Escherichia coli. And the role and molecular mechanism of integron in drug resistance level transmission. Methods automatic bacterial identification / drug sensitivity analyzer was used. Fifteen strains of Escherichia coli isolated from healthy human intestines were tested for their susceptibility to 14 antibiotics and ESBLs interpretation, and 7 antibiotics were detected by disk diffusion method. The coincidence rate of the two drug sensitivity methods was compared by parallel experiments. PCR method was used to detect bacteria carrying class 鈪

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