【摘要】：心血管疾病的研究少不了動(dòng)物模型的參與。利用動(dòng)物模型來模仿人類所患心血管疾病,不僅可以避免人體試驗(yàn)的風(fēng)險(xiǎn),而且可以隨時(shí)復(fù)制疾病的模型,這樣有利于開展研究,使得我們可以從本質(zhì)上研究這種疾病,探討疾病的發(fā)病機(jī)制,并且找出合適的治療方法[1]。目前,急性心肌梗死的病因?qū)W、治療方案及正確地選擇治療藥物已成為心血管疾病研究的重要課題。長期以來實(shí)驗(yàn)室研究是用大鼠制作急性心肌梗死模型,但是國內(nèi)的急性心肌梗死模型的制作存在一定的缺陷和局限性。首先,大部分人是在人工呼吸機(jī)下制作急性心肌梗死模型,在氣管切開時(shí),易出血堵塞呼吸道,增加動(dòng)物的死亡率。同時(shí),由于呼吸機(jī)的型號、參數(shù)調(diào)整以及使用呼吸機(jī)時(shí)給動(dòng)物帶來的創(chuàng)傷等諸多因素,使得實(shí)驗(yàn)的重現(xiàn)性差,得出的數(shù)據(jù)可信程度降低;其次,使用呼吸機(jī)使實(shí)驗(yàn)的時(shí)間會(huì)大大延長,由于實(shí)驗(yàn)室的條件(包括儀器、環(huán)境和溫度等)每天都會(huì)有所不同,從而增大了實(shí)驗(yàn)的系統(tǒng)誤差和實(shí)驗(yàn)開支,使得實(shí)驗(yàn)很難開展。 因此,為了避免上述缺點(diǎn)和局限性,我們采用非人工呼吸機(jī)下開胸結(jié)扎大鼠冠狀動(dòng)脈前降支造成急性心肌梗死模型,通過控制麻藥的劑量、開胸的時(shí)間和減少出血等來提高大鼠的成活率,并且于急性心肌梗死6、12、24及48小時(shí),通過心肌梗死面積、血清CK、LDH及AST活性、全血粘度和血漿粘度等指標(biāo)確定非人工呼吸機(jī)下急性心肌梗死模型建立后各項(xiàng)指標(biāo)的最佳檢測時(shí)間。同時(shí)采用中藥和西藥進(jìn)行藥物干預(yù),以進(jìn)一步確證非人工呼吸機(jī)下急性心肌梗死模型建立的可靠性。
[Abstract]:The study of cardiovascular disease does not involve animal models. The use of animal models to mimic cardiovascular disease in humans not only avoids the risk of human trials, but also replicates models of disease at any time, which facilitates research and allows us to study the nature of the disease. To explore the pathogenesis of the disease and to find out appropriate treatment methods [1]. At present, the etiology of acute myocardial infarction (AMI), treatment regimen and correct choice of treatment drugs have become an important subject of cardiovascular disease research. For a long time, the rat model of acute myocardial infarction has been used in laboratory research, but there are some defects and limitations in the establishment of acute myocardial infarction model in China. First of all, most people make acute myocardial infarction model under artificial ventilator. During tracheotomy, it is easy to bleed and clog the respiratory tract and increase animal mortality. At the same time, because of many factors, such as the type of ventilator, the adjustment of parameters and the trauma caused by the use of ventilator, the reproducibility of the experiment is poor, and the reliability of the obtained data is reduced. Secondly, The use of ventilator can prolong the time of experiment greatly, because the conditions of laboratory (including instrument, environment and temperature etc.) will be different every day, thus increasing the systematic error of experiment and experiment expense, making the experiment very difficult to carry out. Therefore, in order to avoid the shortcomings and limitations mentioned above, we established the model of acute myocardial infarction by ligating the anterior descending branch of coronary artery under non-artificial ventilator in rats, by controlling the dosage of anesthetic. The time of thoracotomy and the reduction of bleeding were used to increase the survival rate of rats, and at 24 and 48 hours after acute myocardial infarction, the area of myocardial infarction, the activity of serum CKT LDH and AST were measured. The parameters of whole blood viscosity and plasma viscosity were used to determine the best detection time after the establishment of acute myocardial infarction model under non-artificial ventilator. Both traditional Chinese medicine and western medicine were used to further confirm the reliability of the model of acute myocardial infarction under non-artificial ventilator.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2006
【分類號】：R-332;R542.22

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