【摘要】：人B淋巴細(xì)胞刺激因子(human B Lymphocyte Stimulator,hBlyS)是一種B淋巴細(xì)胞的共刺激因子,屬TNF超家族成員。BLyS有三個(gè)受體BAFF-R,BCMA和TACI,三個(gè)受體主要表達(dá)于B淋巴細(xì)胞。BLyS在Anti-IgM或IL-4存在下能專一的刺激B細(xì)胞增殖、分化及分泌免疫球蛋白,在體液免疫中有重要的作用。BLyS同自身免疫性疾病有關(guān),在很多的淋巴瘤的發(fā)生中有作用。 嵌合毒素是具有導(dǎo)向能力的分子(載體)和具有細(xì)胞毒性的分子(毒素)偶聯(lián)而成的具有特異性細(xì)胞殺傷能力的雜合分子。載體主要使用抗體及細(xì)胞因子,毒素主要使用超抗原及蛋白質(zhì)合成抑制劑等。嵌合毒素在腫瘤的治療上有其突出的優(yōu)點(diǎn):對(duì)腫瘤細(xì)胞具有選擇性殺傷作用,細(xì)胞毒效價(jià)高,無抗藥性。 本論文詳細(xì)介紹:本室構(gòu)建的重組pET30a/hsBLyS質(zhì)粒在BL21(DE3)菌株中的穩(wěn)定性觀察,嵌合毒素hsBLyS-PE40的基因構(gòu)建、表達(dá)及活性檢測(cè)。
[Abstract]:Human B lymphocyte stimulating factor (human B Lymphocyte StimulatorhBlyS) is a costimulator of B lymphocytes. BLyS, a member of the TNF superfamily, has three receptors, BAFF-RCMA and TACI.The three receptors are mainly expressed in B lymphocytes. BLyS can specifically stimulate the proliferation, differentiation and secretion of immunoglobulin in B cells in the presence of Anti-IgM or IL-4. BLyS plays an important role in humoral immunity. BLyS is associated with autoimmune diseases and plays an important role in the development of lymphoma. Chimeric toxin is a hybrid molecule with specific cytotoxicity, which is conjugated with molecules (vectors) and cytotoxic molecules (toxins). Antibodies and cytokines are used as carriers, superantigens and protein synthesis inhibitors are used as toxins. Chimeric toxin has many advantages in the treatment of tumor: selective killing of tumor cells, high cytotoxicity and no drug resistance. The stability of recombinant pET30a/hsBLyS plasmid in BL21 (DE3) strain, gene construction, expression and activity detection of chimeric toxin hsBLyS-PE40 were described in detail.
【學(xué)位授予單位】：南京師范大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2005
【分類號(hào)】：R392.1

【共引文獻(xiàn)】

相關(guān)博士學(xué)位論文 前1條

1 萬兵兵;人類細(xì)胞生長相關(guān)新基因hOLFML1和C10ORF58的功能研究[D];復(fù)旦大學(xué);2008年

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本文編號(hào)：2187866