【摘要】：第一部分 能量控制法大鼠IUGR模型的建立 【目的】探討能量控制方法建立大鼠IUGR模型的效果,為研究SGA大鼠早期生長發(fā)育提供研究對象。 【方法】選取70天日齡、體重為220～265克的成熟未孕Wistar大鼠,以常規(guī)飼養(yǎng)方法喂養(yǎng),試驗時按雌雄2:1比例合籠交配,次晨取陰道分泌物涂片查精子,查見精子確定為受孕0天。將受孕鼠23只隨機分為限食組和對照組,另選取未孕鼠12只作為未孕限食組。限食組從受孕開始控制進食量,食物量為對照組前一天進食量的一半,直到分娩,分娩后即恢復(fù)到自由進食。孕前和孕期每周測量體重。鼠仔生后由母鼠喂養(yǎng),出生時和生后每3天測量體重和身長。 【結(jié)果】限食組和對照組的孕鼠體重均呈現(xiàn)正性增長,限食組的體重增長明顯低于對照組,而未孕限食組體重卻呈負(fù)增長。限食組和對照組所產(chǎn)鼠仔數(shù)量沒有差異。以對照組鼠仔出生體重作為參照,限食組IUGR鼠的發(fā)生率為51.01%。限食組鼠仔體重在12天以前、身長在15天以前與對照組差異明顯,之后就沒有差異。限食組鼠仔生后1周以內(nèi)體重增長低于對照組、身長增長與對照組無差異,之后體重身長增長都快于對照組。 【結(jié)論】中度食物限制明顯影響孕期的體重增長且懷孕狀態(tài)下的營養(yǎng)代謝模式與非孕期明顯不同,可能與孕期體內(nèi)激素水平的變化有關(guān)。在較好條件下飼養(yǎng)的大鼠受孕時身體儲備較好,早期的中度食物控制對受孕沒有大的影響,一旦受孕,中度食物控制不會增加胚胎和胎鼠的死亡。全程中度食物限制明顯影響
[Abstract]:Part one: the establishment of IUGR model in rats with energy control method [objective] to investigate the effect of energy control method on establishing rat IUGR model. In order to study the early growth and development of SGA rats, 70 days old Wistar rats weighing 220 ~ 265g were selected and fed with conventional feeding methods. In the experiment, the female and male were mated in a cage at 2:1, and the vaginal secretion smear was taken in the morning to check the sperm, and the sperm was determined to be pregnant for 0 days. Twenty-three pregnant rats were randomly divided into restricted group and control group, and 12 non-pregnant rats were selected as control group. The food intake of the restricted group was half of that of the control group the day before, and then returned to free eating after delivery. Weight is measured before and during pregnancy. The body weight and body length were measured at birth and every 3 days after birth. [results] the body weight of pregnant mice in diet restricted group and control group showed positive increase. The weight gain of the restricted diet group was significantly lower than that of the control group, but the weight of the non pregnant diet restricted group was negative. There was no difference in litter size between the diet restricted group and the control group. The birth weight of rats in control group was taken as reference. The incidence of IUGR in diet restricted group was 51. 01%. The body weight of the rats in the diet restricted group was 12 days ago, and the body length was 15 days before the control group, but there was no difference between the diet control group and the control group. The gain of body weight in the diet restricted group was lower than that in the control group within 1 week after birth, and there was no difference in the growth of body length between the control group and the control group. [conclusion] moderate food restriction significantly affected the weight gain during pregnancy and nutritional metabolic patterns during pregnancy. [conclusion] moderate food restriction significantly affects the nutritional metabolic patterns during pregnancy. [conclusion] moderate food restriction significantly affects the weight gain during pregnancy and the nutritional metabolic pattern during pregnancy. Unlike non-pregnant women, It may be associated with changes in hormone levels during pregnancy. The rats fed under better conditions had better body reserves during pregnancy, but early moderate food control had no significant effect on pregnancy. Once pregnant, moderate food control did not increase the mortality of embryo and fetus. Significant effects of moderate food restriction during the whole course
【學(xué)位授予單位】：四川大學(xué)
【學(xué)位級別】：博士
【學(xué)位授予年份】：2005
【分類號】：R-332

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