【摘要】：目的 研究三鄰甲苯基磷酸酯(Tri-ortho-cresyl phosphate，TOCP)誘發(fā)母雞遲發(fā)性神經(jīng)毒性(organophosphorus easter-induced delayed neurotoxicity，OPIDN)發(fā)生過程中(1)坐骨神經(jīng)動作電位(compound action potention parameters，CAP)特性的時(shí)間-效應(yīng)關(guān)系及其與癥狀分級間的關(guān)系；(2)中樞和周圍神經(jīng)系統(tǒng)組織及血清中氧化抗氧化系統(tǒng)時(shí)間-效應(yīng)關(guān)系及其與癥狀分級間的關(guān)系；(3)神經(jīng)組織中凋亡相關(guān)蛋白cyt-c表達(dá)的時(shí)間-效應(yīng)關(guān)系，尋找敏感指標(biāo)，為探討TOCP引起OPIDN的發(fā)病機(jī)制及早期診斷提供依據(jù)。 方法 1．建立動物模型：成年羅曼母雞，隨機(jī)分為d0，5，10，15和21，5個(gè)時(shí)間點(diǎn)。TOCP溶解于玉米油中，給藥組母雞一次po 750mg.kg~(-1) TOCP(0.65ml／kg)，，對照組(d0)給予同體積的玉米油。 2．遲發(fā)性神經(jīng)毒性臨床癥狀觀察：觀察各組母雞的一般狀況變化，定期測量母雞體重，每天觀察母雞的OPIDN臨床表現(xiàn)至實(shí)驗(yàn)結(jié)束，按8級評價(jià)神經(jīng)毒性。 3．痛覺閾值及坐骨神經(jīng)動作電位參數(shù)測定：分別測定d0，5，10，15和21試驗(yàn)動物的痛覺閾值，坐骨神經(jīng)傳導(dǎo)速度，CAP潛伏期、波幅、最大刺激強(qiáng)度和閾強(qiáng)度。 4．神經(jīng)組織和血清中脂質(zhì)過氧化及抗氧化能力指標(biāo)的測定：分別測定d0，5，10，15和21大腦、脊髓、坐骨神經(jīng)和血清中丙二醛(malondialdehyde，MDA)和谷胱甘肽(glutathione，GSH)含量、谷胱甘肽過氧化物酶(glutathione peroxidase，GSH-Px)活性、谷胱甘肽還原酶(glutathione reductase，GR)活性、超氧化物歧化酶(superoxide dismutase，SOD)活性、抗活性氧(anti-reactive oxygen
[Abstract]:Objective to study the time-effect relationship of the characteristics of sciatic nerve action potential (compound action potention parametersCAP) in organophosphorus easter-induced delayed neurotoxicity induced by Tri-ortho-cresyl phosphate TOCP and its relationship with symptom grading in hens. (2) the time-effect relationship of oxidative antioxidant system in central and peripheral nervous system and serum and its relationship with symptom grading, (3) the time-effect relationship of cyt-c expression of apoptosis-related protein in nerve tissue, and the search for sensitive indexes. To investigate the pathogenesis and early diagnosis of OPIDN caused by TOCP. Methods 1.Establishment of animal model: adult Roman hens were randomly divided into five time points. TOCP was dissolved in corn oil. The hens in the administration group received 750 mg 路kg ~ (-1) TOCP (0.65ml/kg once, while the control group (day 0) was given the same volume of corn oil. 2. Observation of the clinical symptoms of delayed neurotoxicity: the observation of each group Changes in the general state of the hens in the group, The weight of hens was measured regularly, and the clinical manifestations of OPIDN were observed every day until the end of the experiment. The threshold of pain and the parameters of sciatic nerve action potential were measured. The conduction velocity of sciatic nerve in the latent period and amplitude of CAP, Maximum stimulation intensity and threshold intensity. 4. Determination of lipid peroxidation and antioxidant capacity in nerve tissue and serum. The contents of malondialdehyde (MDA) and glutathione (GSH), the activity of glutathione peroxidase (GSH-Px) and the activity of glutathione reductase (glutathione reductase gr) in sciatic nerve and serum were measured. Superoxide Dismutase (superoxide) activity and Anti-reactive oxygen species (anti-reactive oxygen) activity
【學(xué)位授予單位】：山東大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2006
【分類號】：R363

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