發(fā)布時間：2018-07-25 17:31

【摘要】：紋狀體是基底神經(jīng)節(jié)接受傳入信息的主要核團(tuán),在中樞神經(jīng)系統(tǒng)有著不可替代的功能。而同源框基因由于其在生物進(jìn)化過程中保持了相對的保守性,并且在神經(jīng)系統(tǒng)有極為廣泛的表達(dá)而成為近年來神經(jīng)科學(xué)領(lǐng)域研究的熱門之一。本實(shí)驗(yàn)針對LIM同源框基因家族的一員islet-1基因進(jìn)行了研究,利用常規(guī)組織學(xué)染色和免疫組織化學(xué)染色等技術(shù)觀察了胚胎第13天至成年大鼠紋狀體發(fā)育過程中islet-1基因的表達(dá)狀況。 結(jié)果顯示:胚胎第13天,大鼠腹側(cè)端腦已存在islet-1蛋白免疫組織化學(xué)反應(yīng)陽性細(xì)胞;在不同發(fā)育時期,紋狀體中islet-1陽性細(xì)胞的數(shù)量存在明顯差異;同一發(fā)育階段中,不同腦區(qū)該基因的表達(dá)狀況亦不相同。 提示:中樞神經(jīng)系統(tǒng)發(fā)育過程中islet-1基因的表達(dá)有顯著的時間和空間差異;紋狀體發(fā)育早期,islet-1蛋白陽性細(xì)胞廣泛的分布說明該基因很可能表達(dá)于不同類型神經(jīng)元前體細(xì)胞;隨著紋狀體逐漸發(fā)育成熟,islet-1基因的表達(dá)逐漸局限于少數(shù)神經(jīng)元中,并可能對神經(jīng)元的表型具備調(diào)節(jié)作用。
[Abstract]:Striatum is the main nucleus of basal ganglion which receives afferent information and has irreplaceable function in the central nervous system. The homobox gene has become one of the hot research fields in the field of neuroscience in recent years due to its relatively conservative nature in biological evolution and its wide expression in the nervous system. In this study, a member of islet-1 gene of LIM homobox gene family was studied. The expression of islet-1 gene during the development of striatum from the 13th day of embryo to adult rat was observed by routine histological staining and immunohistochemical staining. The results showed that there were islet-1 protein immunoreactive cells in the ventral cerebrum of rats on the 13th day of embryo, and there were significant differences in the number of islet-1 positive cells in striatum at different developmental stages. The expression of the gene was also different in different brain regions. The results suggest that there are significant temporal and spatial differences in the expression of islet-1 gene during the development of the central nervous system, and the wide distribution of islet-1 protein positive cells in the early stage of striatum development suggests that the gene may be expressed in different types of neuronal precursor cells. As the striatum matures, the expression of islet-1 gene is restricted to a few neurons and may regulate the phenotype of neurons.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2006
【分類號】：R329.1

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本文編號：2144533