本文選題：人堿性成纖維細胞生長因子(hbFGF) + 大豆��； 參考：《吉林大學》2007年博士論文

【摘要】： 本研究利用基因重組技術在大豆毛狀根中表達人bFGF。將人bFGF基因克隆到質粒pCAMBIA1301載體上，以大豆子葉節(jié)和胚軸為外植體，通過發(fā)根農桿菌C58C1介導轉入到大豆中，，經潮霉素抗性篩選，獲得潮霉素抗性的毛狀根。PCR法擴增檢測人bFGF基因的整合。提取毛狀根中蛋白，用Western blot印跡分析人bFGF的表達。陽性毛狀根中整合并表達人bFGF基因。SDS-PAGE顯示樣品相對分子量約為18kDa，與人bFGF標準品相對分子量一致，證明人bFGF基因在大豆的毛狀根中正確表達。對轉化毛狀根規(guī)�；瘧腋∨囵B(yǎng)條件進行了優(yōu)化，確定了最佳的培養(yǎng)條件，為利用大豆毛狀根作為生物反應器工業(yè)化生產天然藥物奠定實驗基礎。
[Abstract]:The aim of this study was to express human bFGF in soybean hairy roots by gene recombination technique. The human bFGF gene was cloned into plasmid pCAMBIA1301 and transformed into soybean by Agrobacterium tumefaciens C58C1, and was screened by hygromycin resistance. Hygromycin resistant hairy roots were amplified by PCR to detect the integration of human bFGF gene. The protein was extracted from hairy root and the expression of human bFGF was analyzed by Western blot. The expression of human bFGF gene. SDS-PAGE in the positive hairy root showed that the relative molecular weight of the sample was about 18kDa. it was proved that the human bFGF gene was correctly expressed in the hairy root of soybean. The optimal culture conditions of the transformed hairy roots were optimized and the optimum culture conditions were determined, which laid the experimental foundation for the industrialized production of natural drugs by using soybean hairy roots as bioreactor.
【學位授予單位】：吉林大學
【學位級別】：博士
【學位授予年份】：2007
【分類號】：R346

【引證文獻】

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本文編號：2090855