本文選題：兩岐雙岐桿菌 + 鼠傷寒沙門菌　； 參考：《新疆醫(yī)科大學》2006年碩士論文

【摘要】：目的：以鼠傷寒沙門菌(Salmonella typhimurium，STM)感染小鼠為模型，研究兩岐雙岐桿菌(Bifidobacterim bifidum，B．bifidum)在體內對STM感染小鼠的治療作用。方法：先用硫酸鏈霉素給正常小鼠灌胃2d，出現菌群失調癥狀，再用STM灌胃造成小鼠STM感染，使用兩岐雙岐桿菌液干預治療STM感染小鼠，檢測不同治療時間各組小鼠糞便STM菌落數、肝脾指數、血及脾中TNF-a的含量。采用SPSS12.0統(tǒng)計軟件包進行數據處理分析，統(tǒng)計學方法采用雙因素重復測量資料的方差分析、單因素隨機化設計的方差分析、卡方檢驗、多組均數比較的非參數秩和檢驗。結果：隨著治療天數的增加，，STM菌落數逐漸減少，未干預組、生理鹽水組及雙歧桿菌組比較結果均為P＜0.01；組間比較，雙歧桿菌組STM數下降最快。未干預組、生理鹽水組及雙歧桿菌組肝脾指數比較均P＞0.05；STM感染各組與正常組血及脾中TNF-a的含量比較均P＜0.05；STM感染各組中未干預組、生理鹽水組及雙歧桿菌組血中TNF-a的含量比較P＞0.05，脾中TNF-a的含量比較P＜0.05；組間比較，未干預組與雙歧桿菌組比較，P＜0.05。結論：隨著治療天數的增加，STM菌落數逐漸減少，未干預組、生理鹽水組及雙歧桿菌組比較有統(tǒng)計學意義，提示雙歧桿菌對小鼠STM感染有治療作用；根據肝脾指數的比較結果，說明其對免疫器官干預治療作用不夠顯著；正常組、未干預組、生理鹽水組及雙歧桿菌組血及脾TNF-a含量比較表明，STM感染各組血及脾中TNF-a含量均高于正常組，且雙歧桿菌組最高，提示雙歧桿菌干預對感染機體免疫細胞分泌TNF-a有一定促進作用。
[Abstract]:Objective: to study the therapeutic effect of Bifidobacterium bifidum (Bifidobacterium bifidum) on STM infected mice in vivo using Salmonella typhimurium STM as a model. Methods: the normal mice were given streptomycin sulfate for 2 days, and then STM was used to induce STM infection in mice. The STM infected mice were treated with bifidobacterium solution. The fecal STM colony number, liver and spleen index, TNF-a content in blood and spleen were measured. SPSS 12.0 software package was used to process the data, and the statistical method was used to analyze the variance of double-factor repeated measurement data, single factor randomization design analysis of variance, chi-square test, and non-parametric rank sum test of multi-group mean comparison. Results: with the increase of treatment days, the number of STM colonies gradually decreased. The results of no intervention group, normal saline group and Bifidobacterium group were all P < 0.01. The levels of TNF-a in blood and spleen of non-intervention group, normal saline group and bifidobacterium group were significantly higher than those of control group (P > 0.05). The contents of TNF-a in blood and spleen in normal saline group and bifidobacterium group were P > 0.05 and P < 0.05, respectively, and there were significant differences between non-intervention group and Bifidobacterium group (P < 0.05). Conclusion: with the increase of treatment days, the number of STM colonies gradually decreased, and the number of STM colonies in non-intervention group, normal saline group and bifidobacterium group were significantly higher than those in control group, suggesting that Bifidobacterium could treat STM infection in mice, according to the comparison of liver and spleen index. The results showed that TNF-a content in blood and spleen of normal group, non-intervention group, normal saline group and bifidobacterium group was higher than that of normal group, and the TNF-a content in blood and spleen of STM infected group was higher than that of normal group, and that of Bifidobacterium group was the highest. The results suggest that the intervention of Bifidobacterium can promote the secretion of TNF-a by immune cells.
【學位授予單位】：新疆醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2006
【分類號】：R371

【引證文獻】

相關碩士學位論文 前1條

1 吳瓊;復合菌種發(fā)酵豆乳營養(yǎng)成分變化的研究[D];吉林農業(yè)大學;2013年

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