本文選題：深低溫冷藏 + 同種異體骨��； 參考：《福建醫(yī)科大學(xué)》2006年碩士論文

【摘要】： 目的:探討不同時(shí)段深低溫冷藏對(duì)骨組織結(jié)構(gòu)與力學(xué)性能的影響,為臨床同種異體骨移植提供基礎(chǔ)理論。 方法:研究材料為成人脛骨,在相同部位取其骨片、骨段樣本,以15%DMSO為低溫保護(hù)劑,采用兩步冷凍法,即實(shí)驗(yàn)樣本用-55℃的冷凍預(yù)處理,在-196℃液氮中保存2、4、6、8、10、12個(gè)月后,實(shí)驗(yàn)前對(duì)不同時(shí)段的標(biāo)本用40℃水浴快速?gòu)?fù)溫,行光鏡與電鏡觀察、抗壓與抗折的力學(xué)測(cè)試。 結(jié)果:(1)骨的顯微結(jié)構(gòu):在冷藏2、4個(gè)月組的骨細(xì)胞、哈氏系統(tǒng)的中央管接近對(duì)照組;冷藏6個(gè)月之后開始出現(xiàn)骨細(xì)胞減少,中央管擴(kuò)大,有的呈囊樣擴(kuò)張;冷藏2、4、6、8個(gè)月組的骨基質(zhì)保持正常,10個(gè)月組之后出現(xiàn)水腫、小片狀凝固壞死、或玻璃樣變性。(2)骨的超微結(jié)構(gòu):冷藏2、4個(gè)月組的骨細(xì)胞結(jié)構(gòu)正常;冷藏6個(gè)月之后開始出現(xiàn)骨細(xì)胞固縮,冷藏2、4、6個(gè)月組的骨膠原纖維排列整齊,冷藏8個(gè)月組的骨膠原纖維排列模糊不清。(3)骨的力學(xué)測(cè)試:冷藏6個(gè)月組的強(qiáng)度極限、冷藏6個(gè)月組的彈性模量和冷藏10個(gè)月組的壓縮率與對(duì)照組有統(tǒng)計(jì)學(xué)意義上的區(qū)別(P0.05),但各冷藏實(shí)驗(yàn)組的極限荷載與對(duì)照組沒(méi)有統(tǒng)計(jì)學(xué)意義上的區(qū)別(P0.05)。 結(jié)論:(1)骨組織深低溫冷藏隨著冷藏時(shí)間段的延長(zhǎng)出現(xiàn)組織結(jié)構(gòu)進(jìn)行性損傷與力學(xué)性能減退;(2)深低溫冷藏6個(gè)月是骨組織損傷的開始時(shí)段;(3)深低溫冷藏對(duì)骨細(xì)胞與骨基質(zhì)的損傷不在同一時(shí)段,呈先、后出現(xiàn);(4)力學(xué)性能變化晚于骨組織結(jié)構(gòu)的改變。
[Abstract]:Objective: To investigate the effects of deep cryopreservation on bone tissue structure and mechanical properties in different periods, so as to provide basic theory for clinical allograft.
Methods: To study the material for adult tibia, take the bone slices and bone samples in the same part, take 15%DMSO as the cryogenic protective agent, use the two step freezing method, that is, the experimental samples are frozen in -55 C for 2,4,6,8,10,12 months in the liquid nitrogen of -196 C, and the specimens of the different segments are rewarming quickly before the experiment, and the light and electron microscopic views are used. Inspection, mechanical testing of compression and bending.
Results: (1) the microstructure of the bone: the bone cells in the cold storage 2,4 month group, the central tube of the Hart's system was close to the control group. After 6 months of cold storage, the osteocyte decreased, the central canal expanded, and some cystic dilatation; the bone matrix in the cold storage group of 2,4,6,8 months remained normal, and the edema, small slice necrosis, or glass like after the 10 month group were found. (2) ultrastructure of bone: the bone cell structure in the cold storage 2,4 month group was normal; bone cell fixation began to appear after 6 months of cold storage, the bone gelatin fibrils in the cold storage group of 2,4,6 months were arranged orderly, and the collagen fibrils in the 8 month cold storage group were ambiguous. (3) the mechanical test of the bone: the strength limit of the 6 month cold storage group and the 6 month cold storage bomb The compression rate of the sex modulus and the 10 month cold storage group was significantly different from the control group (P0.05), but the limit load of the cold storage experimental groups was not statistically different from that of the control group (P0.05).
Conclusions: (1) the tissue structure progressive injury and mechanical properties decrease with the prolongation of cold storage time, and (2) deep cryogenic cold storage for 6 months is the beginning of bone tissue damage; (3) the damage of bone cells and bone matrix in deep cryogenic cold storage is not at the same time, and appears first and later; (4) changes of mechanical properties are later than those of bone group. The change of fabric structure.
【學(xué)位授予單位】：福建醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2006
【分類號(hào)】：R329

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 林叢,侯寬永,張文秀,呂京寧,公衍道,丁曉嵐,張秀芳,趙南明;深低溫在肝細(xì)胞移植中減輕免疫排斥反應(yīng)的作用[J];北京醫(yī)科大學(xué)學(xué)報(bào);1998年01期

2 王金成,李志洲,陳偉,高中禮,段德生;骨庫(kù)骨的生物力學(xué)特性比較研究[J];骨與關(guān)節(jié)損傷雜志;2001年01期

3 劉勇;同種異體骨移植的免疫反應(yīng)[J];國(guó)外醫(yī)學(xué)(創(chuàng)傷與外科基本問(wèn)題分冊(cè));1998年02期

4 楊維東，，丁鴻才，馬振國(guó)，陳建元，金伯泉;不同時(shí)間深冷凍異體骨移植的免疫原性研究[J];口腔頜面外科雜志;1994年04期

5 張朝春,張發(fā)惠,陳日齊,鄭和平,徐皓;不同時(shí)段超深低溫保存SD大鼠骨組織的生物力學(xué)研究[J];傷殘醫(yī)學(xué)雜志;2004年02期

6 楊克強(qiáng),王臻,桑宏勛,呂昌偉,王海強(qiáng),彭磊,李光新;深低溫冷凍異體骨生物力學(xué)特性與儲(chǔ)存時(shí)間的關(guān)系[J];醫(yī)用生物力學(xué);2000年03期

7 陳振光,張發(fā)惠,謝昀,郭廣松,陶圣祥,楊玉華,潘峰;同種異體骨與血管組織深低溫冷藏的時(shí)限研究[J];中華創(chuàng)傷骨科雜志;2004年09期

8 牛曉輝,徐萬(wàn)鵬,郝林,蔡尤伯,楊榮利;異體骨移植治療骨腫瘤[J];中華骨科雜志;1997年02期

9 陳振光,張發(fā)惠,鄭和平;吻合血管同種異體骨移植的解剖學(xué)研究[J];中華實(shí)驗(yàn)外科雜志;2001年01期

10 張發(fā)惠,陳振光,張朝春,鄭和平,謝昀;不同冷凍預(yù)處理溫度對(duì)大鼠骨、血管超微結(jié)構(gòu)的影響[J];中華實(shí)驗(yàn)外科雜志;2004年05期

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