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  本文選題：深低溫冷藏 + 同種異體骨�。� 參考：《福建醫(yī)科大學》2006年碩士論文

【摘要】： 目的:探討不同時段深低溫冷藏對骨組織結構與力學性能的影響,為臨床同種異體骨移植提供基礎理論。 方法:研究材料為成人脛骨,在相同部位取其骨片、骨段樣本,以15%DMSO為低溫保護劑,采用兩步冷凍法,即實驗樣本用-55℃的冷凍預處理,在-196℃液氮中保存2、4、6、8、10、12個月后,實驗前對不同時段的標本用40℃水浴快速復溫,行光鏡與電鏡觀察、抗壓與抗折的力學測試。 結果:(1)骨的顯微結構:在冷藏2、4個月組的骨細胞、哈氏系統(tǒng)的中央管接近對照組;冷藏6個月之后開始出現(xiàn)骨細胞減少,中央管擴大,有的呈囊樣擴張;冷藏2、4、6、8個月組的骨基質(zhì)保持正常,10個月組之后出現(xiàn)水腫、小片狀凝固壞死、或玻璃樣變性。(2)骨的超微結構:冷藏2、4個月組的骨細胞結構正常;冷藏6個月之后開始出現(xiàn)骨細胞固縮,冷藏2、4、6個月組的骨膠原纖維排列整齊,冷藏8個月組的骨膠原纖維排列模糊不清。(3)骨的力學測試:冷藏6個月組的強度極限、冷藏6個月組的彈性模量和冷藏10個月組的壓縮率與對照組有統(tǒng)計學意義上的區(qū)別(P0.05),但各冷藏實驗組的極限荷載與對照組沒有統(tǒng)計學意義上的區(qū)別(P0.05)。 結論:(1)骨組織深低溫冷藏隨著冷藏時間段的延長出現(xiàn)組織結構進行性損傷與力學性能減退;(2)深低溫冷藏6個月是骨組織損傷的開始時段;(3)深低溫冷藏對骨細胞與骨基質(zhì)的損傷不在同一時段,呈先、后出現(xiàn);(4)力學性能變化晚于骨組織結構的改變。
[Abstract]:Objective: To investigate the effects of deep cryopreservation on bone tissue structure and mechanical properties in different periods, so as to provide basic theory for clinical allograft.
Methods: To study the material for adult tibia, take the bone slices and bone samples in the same part, take 15%DMSO as the cryogenic protective agent, use the two step freezing method, that is, the experimental samples are frozen in -55 C for 2,4,6,8,10,12 months in the liquid nitrogen of -196 C, and the specimens of the different segments are rewarming quickly before the experiment, and the light and electron microscopic views are used. Inspection, mechanical testing of compression and bending.
Results: (1) the microstructure of the bone: the bone cells in the cold storage 2,4 month group, the central tube of the Hart's system was close to the control group. After 6 months of cold storage, the osteocyte decreased, the central canal expanded, and some cystic dilatation; the bone matrix in the cold storage group of 2,4,6,8 months remained normal, and the edema, small slice necrosis, or glass like after the 10 month group were found. (2) ultrastructure of bone: the bone cell structure in the cold storage 2,4 month group was normal; bone cell fixation began to appear after 6 months of cold storage, the bone gelatin fibrils in the cold storage group of 2,4,6 months were arranged orderly, and the collagen fibrils in the 8 month cold storage group were ambiguous. (3) the mechanical test of the bone: the strength limit of the 6 month cold storage group and the 6 month cold storage bomb The compression rate of the sex modulus and the 10 month cold storage group was significantly different from the control group (P0.05), but the limit load of the cold storage experimental groups was not statistically different from that of the control group (P0.05).
Conclusions: (1) the tissue structure progressive injury and mechanical properties decrease with the prolongation of cold storage time, and (2) deep cryogenic cold storage for 6 months is the beginning of bone tissue damage; (3) the damage of bone cells and bone matrix in deep cryogenic cold storage is not at the same time, and appears first and later; (4) changes of mechanical properties are later than those of bone group. The change of fabric structure.
【學位授予單位】：福建醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2006
【分類號】：R329

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