本文選題：人肝再生增強因子 + 基因表達�。� 參考：《浙江大學》2005年碩士論文

【摘要】：前言 各種原因所致的急慢性肝功能衰竭嚴重威脅人類健康,其顯著特點是短期內肝細胞大量嚴重壞死,病死率極高,目前發(fā)病機制尚不清楚,至今尚無有效的藥物治療方法。肝臟是再生能力很強的人體器官,有多種細胞因子參與調節(jié)這一過程,對肝再生調控因子的研究一直是一個熱點,迄今已發(fā)現包括肝細胞生長因子(HGF)、轉化生長因子-α(TGF-α)、表皮生長因子(EGF)、胰島素等二十余種來自肝內外的參與調節(jié)肝再生的刺激因子和抑制因子,但它們均難以解釋器官特異性的肝再生調控機制。1994年Hygiya等從初斷乳大鼠肝組織中克隆得到的一種新型的促肝細胞增殖因子,命名為肝再生增強因子(ALR),肝再生增強因子是目前已知唯一對肝源性細胞有特異性增殖刺激活性的因子,且無種屬特異性,實驗證明該細胞因子能促進肝再生,并對各種急慢性肝損傷有保護作用,在肝損傷或部分肝切除后其表達量明顯提高。 本研究采用分子生物學技術,從正常人肝組織中獲取人肝再生增強因子(hALR)閱讀框全序列,結合pET28a(+)構建hALR原核表達體系,成功地從大腸桿菌中高效而穩(wěn)定地表達出重組人肝再生增強因子蛋白質,并獲高效純化,為進一步進行蛋白質生物學功能鑒定和研制出一種新的抗肝損傷藥物奠定基礎。
[Abstract]:The acute and chronic liver failure caused by various causes is a serious threat to human health. Its remarkable characteristics are a large number of severe necrosis of liver cells in the short term and a high mortality rate. At present, the pathogenesis is not clear, so far there is no effective drug treatment. The liver is a human organ with strong regenerative ability. There are many cytokines involved in the regulation of this process. The research on the regulatory factors of liver regeneration has always been a hot topic. Up to now, more than 20 stimulating and inhibiting factors, including hepatocyte growth factor (HGF), transforming growth factor- 偽 (TGF- 偽), epidermal growth factor (EGFN), insulin and so on, have been found in and out of the liver to regulate liver regeneration. In 1994, Hygiya et al, a novel hepatocyte proliferative factor, was cloned from the liver tissue of the weaned rats, but it was difficult to explain the mechanism of organ specific regulation of liver regeneration. Liver regeneration enhancer is the only known factor that has specific proliferative stimulating activity to hepatogenic cells and has no species-specific. It has been proved that this cytokine can promote liver regeneration. It has protective effect on all kinds of acute and chronic liver injury, and its expression is obviously increased after liver injury or partial hepatectomy. In this study, the whole reading frame sequence of human augmenter of liver regeneration (hALR) was obtained from normal human liver tissue by molecular biology technique, and the prokaryotic expression system of hALR was constructed by pET28a (). The recombinant human liver regeneration enhancer protein was successfully expressed and purified from E. coli, which laid a foundation for further identification of protein biological function and development of a new anti-liver injury drug.
【學位授予單位】：浙江大學
【學位級別】：碩士
【學位授予年份】：2005
【分類號】：R346

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本文編號：2010111