本文選題：碘缺乏 + 碘過(guò)量��； 參考：《鄭州大學(xué)》2005年碩士論文

【摘要】：碘是機(jī)體合成甲狀腺激素(Thyroid Hormone，TH)所必需的微量元素，碘缺乏或碘過(guò)量都將影響TH的合成。TH有許多重要的生理功能，心臟是受TH調(diào)節(jié)的重要靶器官之一，而心肌肌球蛋白重鏈基因則是TH調(diào)節(jié)的重要靶基因。肌球蛋白是心肌主要的結(jié)構(gòu)蛋白，也是起收縮作用的主要蛋白。心肌肌球蛋白重鏈(myosin heavy chain，MHC)包括α和β兩條鏈。然而，碘缺乏和碘過(guò)量能否影響MHC基因的表達(dá)，，尚未見(jiàn)文獻(xiàn)報(bào)道。 本實(shí)驗(yàn)通過(guò)低碘和高碘飼養(yǎng)，造成碘缺乏和碘過(guò)量動(dòng)物模型，觀察其對(duì)正常大鼠心肌α-MHC和β-MHC mRNA表達(dá)的影響。 材料與方法 實(shí)驗(yàn)動(dòng)物：體重120-140g的健康Wistar大鼠90只。將動(dòng)物隨機(jī)分為6組：低碘組(LI)，適量碘組(NI)，5倍碘組(5HI)，10倍碘組(10HI)，50倍碘組(50HI)，100倍碘組(100HI)。每組15只大鼠。 LI組動(dòng)物飼以碘含量小于50μg／kg的低碘飼料，飲用去離子水。NI組和4個(gè)HI組大鼠飼以平均碘含量為300μg／kg的正常大鼠飼料；NI組飲用自來(lái)水，4個(gè)HI組飲用含不同高劑量碘酸鉀(KIO_3)的自來(lái)水。計(jì)算6組大鼠日碘攝入量分別為：＜1μg／d(LI組)、6.15μg／d(NI組)、30.75μg／d(5HI組)、61.5μg／d(10HI組)、307.5μg／d(50HI組)、615μg／d(100HI組)。 大鼠喂養(yǎng)6個(gè)月后，稱(chēng)重，抽血測(cè)定甲狀腺激素，心電圖檢查，股動(dòng)脈放血處死動(dòng)物并迅速摘取心臟，液氮速凍后-80℃保存。 血清甲狀腺激素的測(cè)定：TT_3、TT_4、FT_3、FT_4采用化學(xué)發(fā)光法。 心電圖描記：LI組由于體格瘦小和麻醉易死未進(jìn)行心電圖檢查。NI組和各
[Abstract]:Iodine is an essential trace element in the synthesis of thyroid hormone Thyroid Hormonethra. Iodine deficiency or iodine excess will affect the synthesis of th. Th has many important physiological functions. The heart is one of the important target organs regulated by th. Cardiac myosin heavy chain gene is an important target gene for th regulation. Myosin is the main structural protein of myocardium and the main protein of contraction. Cardiac myosin heavy (MHC) consists of two chains: 偽 and 尾. However, whether iodine deficiency and iodine excess can affect the expression of MHC gene has not been reported. The effects of iodine deficiency and iodine excess on the expression of 偽 -MHC and 尾 -MHC mRNA in myocardium of normal rats were observed. Materials and methods Experimental animals: 90 healthy Wistar rats weighing 120-140 g. The animals were randomly divided into six groups: low iodine group (LI), moderate iodine group (n = 5) with 5 times iodine (n = 5) and 10 times iodine group (n = 10) with 50 times iodine (n = 50) and 100 times iodine group (n = 100). There were 15 rats in each group. The animals of Li group were fed with low iodine diet with iodine content less than 50 渭 g/kg. Rats of deionized water. NI group and 4 HI groups were fed with tap water with average iodine content of 300 渭 g/kg. Four HI groups drank tap water containing different high doses of potassium iodate KIO3). The daily iodine intake was calculated as follows: < 1 渭 g/d(LI group, 6.15 渭 g/d(NI group, 30.75 渭 g/d(5HI group, 61.5 渭 g/d(10HI group, 307.5 渭 g/d(50HI group, 615 渭 g/d(100HI group. After feeding for 6 months, the rats were weighed, blood samples were taken to determine thyroid hormones, electrocardiogram (ECG), femoral artery bloodletting to kill the animals, and the hearts were removed quickly. The rats were preserved at -80 鈩

本文編號(hào)：1981500