本文選題：旋毛形線蟲(chóng) + 地理株��； 參考：《昆明醫(yī)學(xué)院》2006年碩士論文

【摘要】：【目的】 研究旋毛形線蟲(chóng)(云南株)各發(fā)育階段的感染性；探討雄蟲(chóng)對(duì)雌蟲(chóng)生殖能力的影響；通過(guò)光鏡和電鏡對(duì)各感染階段進(jìn)行觀察，從形態(tài)學(xué)角度闡述其感染機(jī)理。 【方法】 138只雄性昆明小鼠，隨機(jī)分為5組(A、B、C、D_(1-7)、E_(1-6))，A、B、C組以成熟囊包幼蟲(chóng)感染，A組于感染后不同時(shí)間剖檢，獲得不同日齡的成蟲(chóng)和幼蟲(chóng)：B組于感染后5d剖檢，確定雌雄性別比例；C組用于計(jì)算成熟囊包幼蟲(chóng)的RCI值。取不同日齡的幼蟲(chóng)感D_(1-7)；取不同日齡的成蟲(chóng)感染E_(1-5)；取單性的5日齡雌蟲(chóng)感染E_6；C、D、E、組均于感染后30天剖檢，計(jì)算RCI值。取不同感染階段的幼蟲(chóng)和成蟲(chóng)固定，用光鏡和電鏡觀察其體表、體壁、桿狀體、食道、生殖器官。 【結(jié)果】 旋毛形線蟲(chóng)(云南株)12、13、14、19日齡幼蟲(chóng)和成熟囊包幼蟲(chóng)的RCI值分別為：49.64±4.02、93.61±4.06、124.34±4.72、153.19±6.20和157.77±3.50；5、10和20日齡成蟲(chóng)的RCI值分別為：154.90±2.62、20.34±4.53和1.18±0.21；5日齡單性雌蟲(chóng)的RCI值為13.77±0.68；11日齡及其以前的幼蟲(chóng)和25日齡及其以后的成蟲(chóng)都不具有感染性。5日成蟲(chóng)的雌雄比例為2.27：1。光鏡結(jié)果顯示：5日齡雌蟲(chóng)子宮內(nèi)蟲(chóng)卵和幼蟲(chóng)密集，，受精囊和卵巢充盈；15日齡雌蟲(chóng)子宮內(nèi)蟲(chóng)卵和幼蟲(chóng)稀少，受精囊萎縮；25日齡成蟲(chóng)子宮內(nèi)未見(jiàn)蟲(chóng)卵和幼蟲(chóng)，受精囊和卵巢結(jié)構(gòu)不清。電鏡觀察：幼蟲(chóng)體表光滑，頭部結(jié)構(gòu)簡(jiǎn)單；不同感染性的成蟲(chóng)和幼蟲(chóng)體壁結(jié)構(gòu)差異較大，桿細(xì)胞中分泌顆粒形態(tài)各異，14日齡幼蟲(chóng)桿細(xì)胞中可見(jiàn)一種新的梭狀的分泌顆粒；各日齡幼蟲(chóng)食道中可見(jiàn)少量非定形物質(zhì)，而成蟲(chóng)食道
[Abstract]:[objective] to study the infection of nematode spiralis (Yunnan strain) at different developmental stages, to explore the effect of male worms on female reproductive ability, to observe the infection stages by light microscope and electron microscope, and to elucidate the mechanism of infection from the point of view of morphology. [methods] 138 male Kunming mice were randomly divided into 5 groups. The adult and larval larvae of group B were dissected at different times after infection. The adult and larvae of group B were dissected at 5 days after infection. The ratio of female to male sex in group C was used to calculate the RCI value of mature cyst larvae. The larvae of different days were infected with D _ (1-7); the adults of different days were infected with Etix 1-5; the unisexual five-day-old females were infected with E6C ~ (2 +). The RCI values were calculated 30 days after infection. The larvae and adults of different infection stages were fixed, and the surface, wall, rodlike body, esophagus and reproductive organs were observed by light and electron microscopy. [results] the RCI values of nematode nematodes (121213A1419-day-old larvae and mature cysts larvae) were: 49.64 鹵4.02293.61 鹵4.06124.34 鹵4.72153.19 鹵6.20 and 157.77 鹵3.50510 鹵6.20 respectively. The RCI values of adult larvae were: 1 154.90 鹵2.62N 20.34 鹵4.53 and 1.18 鹵0.215-day-old female larvae 13.77 鹵0.6811 days old and their RCI values were 1.18 鹵0.215-day-old female larvae of 13.77 鹵0.6811 days old and their RCI values were 1.18 鹵0.21 5 days old female larvae were 13.77 鹵0.6811 days old and their RCI values were 13.77 鹵0.6811 days old. The male and female ratio of the 5 day adult was 2.27: 1. The results of light microscope showed that the eggs and larvae were dense in the womb of female worms of 5 days old, the eggs and larvae were rare in the womb of 15 days old female with filling with seminal vesicle and ovary, and there were no eggs and larvae in the womb of 25 days old adult worms with seminal vesicle atrophy. The structure of seminal vesicle and ovary is unclear. Electron microscope observation showed that the surface of larva was smooth, the structure of head was simple, the structure of body wall of adult and larva with different infection was different, and a new kind of secretory granules could be found in bar cells of 14-day-old larva. A small amount of amorphous substances were found in the esophagus of each day old larva, while the adult oesophagus
【學(xué)位授予單位】：昆明醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2006
【分類(lèi)號(hào)】：R383

【引證文獻(xiàn)】

相關(guān)碩士學(xué)位論文 前1條

1 高云;三苯雙脒對(duì)小鼠感染3個(gè)不同地理株旋毛蟲(chóng)的療效觀察[D];承德醫(yī)學(xué)院;2012年

本文編號(hào)：1967068