本文選題：脂肪來源基質(zhì)細胞 + 多分化潛能　； 參考：《吉林大學(xué)》2007年碩士論文

【摘要】： 脂肪來源的基質(zhì)細胞(adipose derived stromal cells, ADSCs)是近年來從脂肪抽吸物中發(fā)現(xiàn)的一類成體干細胞,與較早被認識的成體干細胞骨髓基質(zhì)細胞(bone marrow stromal cells, BMSCs)有很多相似的特性,并且脂肪組織來源豐富,脂肪抽吸術(shù)簡單易行,自體移植時無免疫排斥等諸多優(yōu)點。 本研究建立了人脂肪脂肪來源基質(zhì)細胞(hADSCs)的分離和擴增方法,探討ADSCs向脂肪細胞方向分化的潛能。采用膠原酶消化法分離擴增人ADSCs,測定其生長的經(jīng)時變化、累積生長倍數(shù)等;應(yīng)用不同因子誘導(dǎo)ADSCs向成脂細胞分化并進行鑒定。結(jié)果表明,每100ml脂肪抽吸物中可分離得到2×107～4×107個有核細胞,60d內(nèi)擴增至第8代;油紅O染色、免疫細胞化學(xué)染色及RT-PCR證實ADSCs經(jīng)誘導(dǎo)可分化為脂肪細胞。ADSCs有望作為一種成體多能干細胞應(yīng)用于組織工程及細胞治療。
[Abstract]:Adipose derived stromal cells, ADSCs) is a kind of adult stem cells found in adipose aspirates in recent years. It has many similar characteristics with bone marrow stromal cells, BMSCs), a bone marrow stromal cell of adult stem cells known earlier. There are many advantages such as abundant adipose tissue sources, simple and easy fat aspiration, no immune rejection in autologous transplantation and so on. In this study, we established a method for the isolation and amplification of human adipose derived stromal cells (hADSCs), and explored the potential of ADSCs to differentiate into adipocytes. Human ADSCs were isolated and amplified by collagenase digestion, their growth time changes and cumulative growth times were measured, and different factors were used to induce ADSCs to differentiate into adipogenic cells and identify them. The results showed that 2 脳 10 ~ 7 ~ 4 脳 10 ~ 7 nucleated cells could be isolated from each 100ml fat aspirate and amplified to the 8th passage within 60 days. Immunocytochemical staining and RT-PCR confirmed that ADSCs could differentiate into adipocytes. ADSCs could be used as a kind of adult pluripotent stem cells in tissue engineering and cell therapy.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2007
【分類號】：R329

【參考文獻】

相關(guān)期刊論文 前1條

1 孫昭,姜學(xué)英,趙春華;脂肪間充質(zhì)干細胞的研究進展[J];國外醫(yī)學(xué).輸血及血液學(xué)分冊;2004年03期

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本文編號：1946198