本文選題：椎間盤 + 椎間盤退變　； 參考：《四川大學》2007年碩士論文

【摘要】： 【研究背景】 椎間盤退變的動物模型是研究椎間盤退變性疾病發(fā)病機制的一種重要途徑，同時為修復退變椎間盤的治療研究提供良好的實驗載體。許多學者選擇不同的動物、通過不同的方法建立了椎間盤退變的動物模型。按退變發(fā)生的原因可將其分為兩大類，即誘發(fā)性和自發(fā)性椎間盤退變模型。前者是通過實驗的方法誘發(fā)椎間盤退變，可按實驗方法的不同進一步分為3種：①改變椎間盤生物力學環(huán)境誘發(fā)退變；②損傷椎間盤自身結(jié)構誘發(fā)退變；③通過基因敲除技術制作退變模型。其中手術直接損傷型椎間盤退變模型，是目前較為普遍采用的一種建模方法，雖然其已廣泛地應用于椎間盤退變的各個研究領域當中，但對不同手術損傷方式所誘導的退變模型進行比較的研究尚少見，對于不同椎間盤退變模型的選擇仍有許多不同意見。 【研究目的】 1．建立不同手術損傷方式所誘導的兔椎間盤退變模型，，并比較其退變過程及其特點； 2．為在椎間盤退變性疾病的研究中，更為合理地選擇與自然退變過程相似和具有可行性、可重復性的動物模型提供理論依據(jù)。 【材料與方法】 本實驗選用健康新西蘭兔20只，采用隨機對照的實驗設計方案，使用三種不同的方法(纖維環(huán)穿刺、髓核抽吸、終板破壞)建立椎間盤退變的動物模型，分批在術后第2、4、8、16、和32周等時間點處死實驗動物并獲取椎間盤標本。通過檢測髓核組織水和硫酸化糖胺多糖(s-GAG)含量的改變，同時結(jié)合影像學檢查(CR片測量椎間盤高度，MRI T2WI評估退變程度)，對不同方法建立的椎間盤退變動物模型的退變過程及其特點進行觀察和比較。所有實驗數(shù)據(jù)應用SPSS 13.0統(tǒng)計學軟件，t檢驗和兩兩比較的方差分析。 【結(jié)果】 根據(jù)髓核組織水和硫酸化糖胺多糖(s-GAG)含量的測量結(jié)果，同時結(jié)合影像學檢查(CR片測量椎間盤高度，MRI T2WI評估退變程度)的結(jié)果，證實三種手術損傷方式均成功地建立了椎間盤退變模型。同時各項指標均呈現(xiàn)與自然椎間盤退變過程相類似的隨時間延長而變化的趨勢。但終板破壞和髓核抽吸所誘發(fā)的椎間盤退變出現(xiàn)在2周后，而纖維環(huán)穿刺所誘發(fā)的椎間盤退變則出現(xiàn)在4周左右。 【結(jié)論】 終板破壞和髓核抽吸型退變模型所誘發(fā)的退變過程出現(xiàn)較早，進展較快且退變程度較重，適合用于對中晚期退變的相關研究。而纖維環(huán)穿刺法誘發(fā)的退變過程較為緩慢，且退變程度與穿刺損傷程度呈相關性，適合用于對早期退變的相關研究
[Abstract]:[research background] The animal model of disc degeneration is an important way to study the pathogenesis of degenerative disc disease and provides a good experimental carrier for the treatment of degenerative disc repair. Many scholars choose different animals and establish animal models of disc degeneration by different methods. According to the causes of degeneration, it can be divided into two categories: induced and spontaneous disc degeneration models. The former can be further divided into 3 kinds according to the different experimental methods, which are induced by experimental method, and can be further divided into 3 kinds: 1. Change the biomechanical environment of intervertebral disc to induce degeneration. 3 the degenerative model was made by gene knockout technique. Surgical direct injury model of disc degeneration is a widely used modeling method, although it has been widely used in various research fields of disc degeneration. However, it is rare to compare the models of degeneration induced by different surgical injury methods, and there are still many different opinions on the choice of different models of intervertebral disc degeneration. [purpose of research] 1. The degenerative model of rabbit intervertebral disc induced by different surgical injury methods was established, and the process and characteristics of degeneration were compared. 2. It provides a theoretical basis for selecting more reasonable animal models which are similar to natural degeneration in the study of degenerative intervertebral disc disease and are feasible and reproducible. [materials and methods] In this experiment, 20 healthy New Zealand rabbits were used to establish the animal model of intervertebral disc degeneration using three different methods (annular puncture, aspiration of nucleus pulposus, destruction of endplate). The experimental animals were killed and the intervertebral disc specimens were collected at the 2nd week and the 32nd week after operation. The changes of water and sulfated glycosaminoglycan (S-GAG) contents in nucleus pulposus were measured. At the same time, the degenerative degree of intervertebral disc was evaluated by MRI T2WI combined with CR film. The degenerative process and characteristics of the animal model of degenerative intervertebral disc were observed and compared. All experimental data were tested by SPSS 13.0 statistical software / t test and anatomic analysis of variance (ANOVA) of pairwise comparison. [results] According to the results of measurement of water and sulfated glycosaminoglycans in nucleus pulposus tissue, combined with imaging examination, the degree of degeneration was evaluated by MRI T2WI of intervertebral disc height measured by CR film. It was proved that the models of intervertebral disc degeneration were established successfully in all three kinds of surgical injury modes. At the same time, all indexes showed a trend of change with time similar to the process of degeneration of natural intervertebral disc. However, disc degeneration induced by endplate destruction and aspiration of nucleus pulposus occurred 2 weeks later, while disc degeneration induced by fiber ring puncture appeared at about 4 weeks. [conclusion] The degenerative process induced by end-plate destruction and aspiration degeneration model of nucleus pulposus appeared earlier, advanced quickly and had a severe degree, so it was suitable for the study of degeneration in the middle and late stages. However, the process of degeneration induced by fiber ring puncture is slow, and the degree of degeneration is correlated with the degree of puncture injury, which is suitable for the study of early degeneration.
【學位授予單位】：四川大學
【學位級別】：碩士
【學位授予年份】：2007
【分類號】：R681.53;R-332

【共引文獻】

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