本文選題：B族鏈球菌 + 脆弱類桿菌�。� 參考：《第一軍醫(yī)大學(xué)》2005年博士論文

【摘要】：包括共生菌在內(nèi)的微生物遍布于人體的體表(如皮膚)或粘附于粘膜上皮組織(如胃腸道、呼吸道、陰道等處)。它們的總數(shù)遠(yuǎn)遠(yuǎn)超過(guò)人體細(xì)胞總數(shù)。在與其宿主長(zhǎng)期共同演化的過(guò)程中,這些微生物發(fā)展了種類繁多的共生或致病因子和手段,以便于它們與宿主相互作用并引起一系列生物學(xué)反應(yīng),從而使得它們更好地侵入宿主細(xì)胞,并在其中長(zhǎng)期生存,繁殖,甚至在某些情況下還引起宿主細(xì)胞癌變。因而,鑒定這些共生于人類的病原微生物的致病因子并研究其分子致病機(jī)理,以及對(duì)病原體進(jìn)行早期診斷是有效預(yù)防和治療其感染的關(guān)鍵所在。本研究綜合應(yīng)用細(xì)菌遺傳學(xué)、細(xì)胞生物學(xué)、生物化學(xué)與分子生物學(xué)以及免疫學(xué)等多學(xué)科的研究方法,首先對(duì)兩個(gè)重要的人體共生病原微生物B族鏈球菌(group B Streptococcus,GBS)和脆弱類桿菌(B.fragilis)的分子致病機(jī)理進(jìn)行了研究;然后以重要致癌病原體人乳頭瘤病毒(human papillomavirus,HPV)為對(duì)象,探索了限制性顯示(restriction display,RD)和DNA芯片技術(shù)在病原體分子診斷中的應(yīng)用前景。 B族鏈球菌(GBS)是一種革蘭式陽(yáng)性鏈球菌。它共生于多數(shù)人類胃腸道,并且也是引起新生兒膿血癥,腦膜炎和肺炎等致命性感染的主要病原體。GBS的莢膜多糖(capsular polysaccahride,CPS)不僅是其主要的致病決定因子,而
[Abstract]:Microbes, including symbiotic bacteria, are found on the body's surface (e.g. skin) or on mucosal epithelium (e.g., gastrointestinal tract, respiratory tract, vagina, etc.). Their total number far exceeds the total number of human cells. In the course of long-term co-evolution with their host, these microbes have developed a wide variety of symbiotic or pathogenic factors and means to facilitate their interaction with the host and to trigger a series of biological responses, It makes them invade host cells better and survive, reproduce and even cause carcinogenesis of host cells in some cases. Therefore, the key to effectively prevent and treat the infection is to identify the pathogenetic factors of these pathogenic microorganisms, to study their molecular pathogenic mechanism, and to make early diagnosis of pathogens. In this study, a comprehensive application of bacterial genetics, cell biology, biochemistry and molecular biology, as well as immunology and other multidisciplinary research methods, The molecular pathogenesis of two important human symbiotic pathogens, group B Streptococcus sp. GBSand B. fragilis, was studied, and then the human papillomavirus HPVs, an important carcinogenic pathogen, were studied. The application prospect of restrictive display restriction (RDX) and DNA chip technology in pathogen molecular diagnosis was explored. Group B streptococcus (GBS) is a Gram-positive streptococcus. It is symbiotic in the gastrointestinal tract of most humans and is also a major pathogen of neonatal sepsis, meningitis and pneumonia. The capsule polysaccharide encapsular capsular polysaccahridea (CPS) is not only its main pathogenic determinant, but also a major pathogen of neonatal sepsis, meningitis and pneumonia.
【學(xué)位授予單位】：第一軍醫(yī)大學(xué)
【學(xué)位級(jí)別】：博士
【學(xué)位授予年份】：2005
【分類號(hào)】：R37

【參考文獻(xiàn)】

相關(guān)會(huì)議論文 前1條

1 ;農(nóng)業(yè)生物化學(xué)與分子生物學(xué)進(jìn)展[A];科技進(jìn)步與學(xué)科發(fā)展——“科學(xué)技術(shù)面向新世紀(jì)”學(xué)術(shù)年會(huì)論文集[C];1998年

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本文編號(hào)：1850519