本文選題：骨髓間充質(zhì)干細(xì)胞 + 表皮干細(xì)胞��； 參考：《中南大學(xué)》2006年博士論文

【摘要】：背景：燒傷、創(chuàng)傷和整形外科的創(chuàng)面修復(fù)需要大量自體皮膚，組織工程化皮膚是解決自體皮來源的良好方法。理想的組織工程化皮膚應(yīng)該是能在盡量短的時(shí)間內(nèi)合成包含有表皮、真皮和各種皮膚附件(汗腺、毛囊、皮脂腺等)以及黑色素細(xì)胞和朗格罕氏細(xì)胞的永久性覆蓋的皮膚，但至今還沒有任何一種組織工程皮膚能作為理想的皮膚覆蓋物，其中種子細(xì)胞和支架材料是組織工程研究的關(guān)鍵。表皮干細(xì)胞具有多分化潛能，除再生皮膚的多層表皮結(jié)構(gòu)外，還形成汗腺、毛發(fā)和毛囊等皮膚附屬器官，但由于目前獲取與純化表皮干細(xì)胞較為困難，從而妨礙了表皮干細(xì)胞作為組織工程化皮膚種子細(xì)胞的應(yīng)用。本研究以HaCaT細(xì)胞培養(yǎng)上清液聯(lián)合EGF作為誘導(dǎo)條件，觀測(cè)BMSCs經(jīng)誘導(dǎo)后的細(xì)胞形態(tài)學(xué)及細(xì)胞生物化學(xué)的變化，以明確BMSCs能否在體外誘導(dǎo)分化為表皮干細(xì)胞；通過以BrdU標(biāo)記BMSCs后，植入全層皮膚缺損創(chuàng)面，觀察其在皮膚缺損創(chuàng)面愈合中的作用，了解骨髓間充質(zhì)干細(xì)胞能否在體內(nèi)誘導(dǎo)分化表皮干細(xì)胞；并觀察其在明膠／聚己酸內(nèi)酯電紡納米支架中生長(zhǎng)的情況。為選用骨髓間充質(zhì)干細(xì)胞作為組織工程化皮膚的種子細(xì)胞提供理論依據(jù)。 第一章：兔自體骨髓間充質(zhì)干細(xì)胞促進(jìn)創(chuàng)面愈合的實(shí)驗(yàn)研究 目的：觀察骨髓間充質(zhì)干細(xì)胞(Bone Mesenchymal Stem Cells，BMSCs)在皮膚缺損創(chuàng)面愈合中的作用，，探討其作為種子細(xì)胞修復(fù)與重建皮膚的可能性。 方法：取成年家兔30只，抽取骨髓，采用直接貼壁法分離、純化和培養(yǎng)骨髓間充質(zhì)干細(xì)胞；采用原位雜交試劑盒對(duì)培養(yǎng)細(xì)胞進(jìn)行cDNA探針原位雜交檢測(cè)CD44，鑒定其為骨髓間充質(zhì)干細(xì)胞；于兔背部?jī)蓚?cè)制造全層皮膚缺損創(chuàng)面，以Ⅰ型膠原為載體，將已用5-溴脫氧尿嘧啶(5-bromodeoxy-uridine，BrdU)標(biāo)記的自體BMSCs植于左側(cè)創(chuàng)面，
[Abstract]:Background: the repair of burn, trauma and orthopedic wounds requires a large number of autologous skin. Tissue engineered skin is a good method to solve the source of autogenous skin. Ideal tissue engineered skin should be permanently covered with epidermis, dermis and various skin attachments (sweat glands, hair follicles, sebaceous glands, etc.) and melanocytes and Langerhans cells in as short a time as possible. However, no tissue engineered skin can be used as an ideal skin covering, and seed cells and scaffolds are the key to tissue engineering. Epidermal stem cells have the potential of multi-differentiation. Besides the multi-layer epidermal structure of regenerated skin, epidermal stem cells also form sweat glands, hair and hair follicles. However, it is difficult to obtain and purify epidermal stem cells. This hinders the application of epidermal stem cells as seed cells of tissue engineering skin. In this study, the supernatant of HaCaT cell culture combined with EGF was used as the induction condition to observe the changes of cell morphology and cell biochemistry of BMSCs after induction, to determine whether BMSCs could be induced to differentiate into epidermal stem cells in vitro, and to determine whether BMSCs could be induced into epidermal stem cells by BrdU labeled BMSCs. To investigate whether the bone marrow mesenchymal stem cells can induce the differentiation of epidermal stem cells in vivo, the effect of the whole skin defect wound was observed. Its growth in gelatin / polycaprolactone electrospun nano-scaffold was observed. To provide theoretical basis for selecting bone marrow mesenchymal stem cells as seed cells of tissue engineered skin. Chapter 1: experimental study on promoting wound healing by autologous bone marrow mesenchymal stem cells in rabbits Objective: to observe the role of bone Mesenchymal Stem cells (BMSCs) in the healing of skin defect wounds, and to explore the possibility of repairing and reconstructing the skin as seed cells of bone marrow mesenchymal stem cells (BMSCs). Methods: bone marrow was extracted from 30 adult rabbits. Bone marrow mesenchymal stem cells were purified and cultured by direct adherent method. CD44 was detected by in situ hybridization with cDNA probe and identified as bone marrow mesenchymal stem cells by in situ hybridization kit. Autologous BMSCs labeled with 5-bromodeoxy-uridine (BrdU) was implanted on the left wound.
【學(xué)位授予單位】：中南大學(xué)
【學(xué)位級(jí)別】：博士
【學(xué)位授予年份】：2006
【分類號(hào)】：R329.2

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 方林森,余又新;表皮干細(xì)胞研究進(jìn)展[J];蚌埠醫(yī)學(xué)院學(xué)報(bào);2005年02期

2 呼和塔娜,郭麗,陳強(qiáng),王建輝,尹飛,李鵬,范洪學(xué);骨髓間充質(zhì)干細(xì)胞治療皮膚深Ⅱ度燒傷的實(shí)驗(yàn)研究[J];吉林大學(xué)學(xué)報(bào)(醫(yī)學(xué)版);2005年03期

3 任杰;張乃文;;具有細(xì)胞識(shí)別信號(hào)的組織工程支架材料的研究[J];材料導(dǎo)報(bào);2004年01期

4 朱堂友,伍津津,胡浪,李文維,賀萍,楊宏珍,張民眾,徐春蓉;殼多糖-膠原-糖胺聚糖凝膠人工皮膚的制備[J];重慶醫(yī)學(xué);2002年10期

5 竺亞斌,高長(zhǎng)有,計(jì) 劍,沈家驄;聚己內(nèi)酯的表面光化學(xué)接枝改性以及細(xì)胞相容性[J];材料研究學(xué)報(bào);2002年03期

6 李明忠,盧神州,張長(zhǎng)勝,吳徵宇;明膠/絲素共混多孔膜的制備[J];東華大學(xué)學(xué)報(bào)(自然科學(xué)版);2002年03期

7 李習(xí)玲,連小華,楊力,楊恬;AnnexinⅡ在已分化人表皮角質(zhì)形成細(xì)胞中表達(dá)的研究[J];第三軍醫(yī)大學(xué)學(xué)報(bào);2005年17期

8 鄧政興,張志雄,李立華,周長(zhǎng)忍;聚乙烯吡咯烷酮接枝殼聚糖的生物相容性評(píng)價(jià)[J];第一軍醫(yī)大學(xué)學(xué)報(bào);2004年06期

9 陳文娜,楊建,王身國,貝建中;聚丙交酯/聚乙二醇多嵌段共聚物的合成及其性能[J];高分子學(xué)報(bào);2002年05期

10 王琴梅,潘仕榮,張靜夏;聚谷氨酸芐酯-聚乙二醇嵌段共聚物的合成和表征[J];功能高分子學(xué)報(bào);2001年01期

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